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. 1981 Feb 1;193(2):615–620. doi: 10.1042/bj1930615

Isolation of an actin-binding fragment of fibronectin.

J Keski-Oja, K M Yamada
PMCID: PMC1162640  PMID: 7030313

Abstract

We have identified a specific actin-binding site in the adhesive glycoprotein fibronectin, isolated from chicken fibroblasts. Affinity chromatography of fragments, released from fibronectin by limited proteolysis with trypsin, chymotrypsin and subtilisin, on actin-Sepharose and other protein-Sepharose columns was used to locate the binding site. A 27 000-mol.wt. subtilisin-digest fragment bound efficiently to actin. The results suggest that the actin-binding site is close to, but not identical with, the reported collagen-binding site.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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