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. 1981 Mar 1;193(3):887–890. doi: 10.1042/bj1930887

Immunoelectrophoretic studies on human small-intestinal brush-border proteins.

H Skovbjerg
PMCID: PMC1162681  PMID: 6118134

Abstract

The amounts of lactase (beta-D-galactosidase, EC 3.2.1.23), sucrase (sucrose alpha-D-glucohydrolase, EC 3.2.1.48), maltase (alpha-D-glucosidase, EC 3.2.1.20) microvillus aminopeptidase (EC 3.4.11.2) and dipeptidyl peptidase IV (EC 3.4.14.-) in tangentially sectioned biopsies from jejunum were studied by quantitative immunoelectrophoresis and enzymic assays. All enzymes had their maximum activities near the mid-region of the villi and their lowest activities at the bases of the crypts. The ratio between enzyme activity and immunoreactive protein was constant along the villus-crypt axis. This result is consistent with a continuous brush-border-enzyme synthesis as the enterocytes migrate up the villi.

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Selected References

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  1. Alpers D. H., Tedesco F. J. The possible role of pancreatic proteases in the turnover of intestinal brush border proteins. Biochim Biophys Acta. 1975 Aug 5;401(1):28–40. doi: 10.1016/0005-2736(75)90338-7. [DOI] [PubMed] [Google Scholar]
  2. Dahlqvist A. Assay of intestinal disaccharidases. Anal Biochem. 1968 Jan;22(1):99–107. doi: 10.1016/0003-2697(68)90263-7. [DOI] [PubMed] [Google Scholar]
  3. Dubs R., Gitzelmann R., Steinmann B., Lindenmann J. Catalytically inactive sucrase antigen of rabbit small intestine: the enzyme precursor. Helv Paediatr Acta. 1975 May;30(1):89–102. [PubMed] [Google Scholar]
  4. Gratecos D., Knibiehler M., Benoit V., Sémériva M. Plasma membranes from rat intestinal epithelial cells at different stages of maturation. I. Preparation and characterization of plasma membrane subfractions originating from crypt cells and from villous cells. Biochim Biophys Acta. 1978 Oct 4;512(3):508–524. doi: 10.1016/0005-2736(78)90161-x. [DOI] [PubMed] [Google Scholar]
  5. James W. P., Alpers D. H., Gerber J. E., Isselbacher K. J. The turnover of disaccharidases and brush border proteins in rat intestine. Biochim Biophys Acta. 1971 Feb 23;230(2):194–203. doi: 10.1016/0304-4165(71)90204-2. [DOI] [PubMed] [Google Scholar]
  6. Nordström C., Dahlqvist A., Josefsson L. Quantitative determination of enzymes in different parts of the villi and crypts of rat small intestine. Comparison of alkaline phosphatase, disaccharidases and dipepeptidases. J Histochem Cytochem. 1967 Dec;15(12):713–721. doi: 10.1177/15.12.713. [DOI] [PubMed] [Google Scholar]
  7. Nordström C., Dahlqvist A. Quantitative distribution of some enzymes along the villi and crypts of human small intestine. Scand J Gastroenterol. 1973;8(5):406–416. [PubMed] [Google Scholar]
  8. Raul F., Simon P., Kedinger M., Haffen K. Intestinal enzymes activities in isolated villus and crypt cells during postnatal development of the rat. Cell Tissue Res. 1977 Jan 12;176(2):167–178. doi: 10.1007/BF00229460. [DOI] [PubMed] [Google Scholar]
  9. Simon P. M., Kedinger M., Raul F., Grenier J. F., Haffen K. Developmental pattern of rat intestinal brush-border enzymic proteins along the villus--crypt axis. Biochem J. 1979 Feb 15;178(2):407–413. doi: 10.1042/bj1780407. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Sjöström H., Norén O., Jeppesen L., Staun M., Svensson B., Christiansen L. Purification of different amphiphilic forms of a microvillus aminopeptidase from pig small intestine using immunoadsorbent chromatography. Eur J Biochem. 1978 Aug 1;88(2):503–511. doi: 10.1111/j.1432-1033.1978.tb12476.x. [DOI] [PubMed] [Google Scholar]
  11. Skovbjerg H., Norén O., Sjöström H. Immunoelectrophoretic studies on human small intestinal brush border proteins. A qualitative study of the protein composition. Scand J Clin Lab Invest. 1978 Dec;38(8):723–729. doi: 10.1080/00365517809104879. [DOI] [PubMed] [Google Scholar]
  12. Wang C., Smith R. L. Lowry determination of protein in the presence of Triton X-100. Anal Biochem. 1975 Feb;63(2):414–417. doi: 10.1016/0003-2697(75)90363-2. [DOI] [PubMed] [Google Scholar]
  13. Webster H. L., Harrison D. D. Enzymic activities during the transformation of crypt to columnar intestinal cells. Exp Cell Res. 1969 Aug;56(2):245–253. doi: 10.1016/0014-4827(69)90009-3. [DOI] [PubMed] [Google Scholar]
  14. Weiser M. M. Intestinal epithelial cell surface membrane glycoprotein synthesis. I. An indicator of cellular differentiation. J Biol Chem. 1973 Apr 10;248(7):2536–2541. [PubMed] [Google Scholar]

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