Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1981 Mar 1;193(3):997–1004. doi: 10.1042/bj1930997

DNA polymerase of a basidiomycete fungus, Coprinus cinereus.

D Lowe, D Lewis
PMCID: PMC1162695  PMID: 7197920

Abstract

DNA polymerase activity was studied in Coprinus cinereus, a basidiomycete fungus. Only one from of the enzyme could be demonstrated, whether by affinity or ion-exchange chromatography; this enzyme had a molecular weight of 185000 on Sephadex G-200, and was inhibited by mercaptoethanol. Coprinus, a representative of the most advanced type of the filamentous fungi, resembles other eukaryotic micro-organisms in its lack of a mammalian beta-type DNA polymerase. The properties of the polymerase are compared with those of two other fungi, and found to resemble most closely the yeast polymerase A in Mg2+ requirements and template preference.

Full text

PDF
997

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adams R. L., Henderson M. A., Wood W., Lindsay J. G. Multiple forms of nuclear deoxyribonucleic acid polymerases and their relationship with the soluble enzyme. Biochem J. 1973 Feb;131(2):237–246. doi: 10.1042/bj1310237. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Banks G. R., Holloman W. K., Kairis M. V., Spanos A., Yarranton G. T. A DNA polymerase from Ustilago maydis. 1. Purification and properties of the polymerase activity. Eur J Biochem. 1976 Feb 2;62(1):131–142. doi: 10.1111/j.1432-1033.1976.tb10106.x. [DOI] [PubMed] [Google Scholar]
  4. Banks G. R., Yarranton G. T. A DNA polymerase from Ustilago maydis. 2. Properties of the associated deoxyribonuclease activity. Eur J Biochem. 1976 Feb 2;62(1):143–150. doi: 10.1111/j.1432-1033.1976.tb10107.x. [DOI] [PubMed] [Google Scholar]
  5. Chang L. M., Bollum F. J. A comparison of associated enzyme activities in various deoxyribonucleic acid polymerases. J Biol Chem. 1973 May 25;248(10):3398–3404. [PubMed] [Google Scholar]
  6. Chang L. M., Bollum F. J. Antigenic relationships in mammalian DNA polymerase. Science. 1972 Mar 10;175(4026):1116–1117. doi: 10.1126/science.175.4026.1116. [DOI] [PubMed] [Google Scholar]
  7. Chang L. M. DNA polymerases from bakers' yeast. J Biol Chem. 1977 Mar 25;252(6):1873–1880. [PubMed] [Google Scholar]
  8. Chang L. M. Phylogeny of DNA polymerase-beta. Science. 1976 Mar 19;191(4232):1183–1185. doi: 10.1126/science.769158. [DOI] [PubMed] [Google Scholar]
  9. Craig R. K., Keir H. M. Deoxyribonucleic acid polymerases of BHK-21/C13 cells. Partial purification and characterization of the enzymes. Biochem J. 1975 Feb;145(2):215–224. doi: 10.1042/bj1450215. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Crerar M., Pearlman R. E. DNA polymerase activity from Tetrahymena pyriformis. FEBS Lett. 1971 Nov 1;18(2):231–237. doi: 10.1016/0014-5793(71)80452-0. [DOI] [PubMed] [Google Scholar]
  11. D'Alesandro M. M., Jaskot R. H., Dunham V. L. Soluble and chromatin-bound DNA polymerases in developing soybean. Biochem Biophys Res Commun. 1980 May 14;94(1):233–239. doi: 10.1016/s0006-291x(80)80211-7. [DOI] [PubMed] [Google Scholar]
  12. De Lucia P., Cairns J. Isolation of an E. coli strain with a mutation affecting DNA polymerase. Nature. 1969 Dec 20;224(5225):1164–1166. doi: 10.1038/2241164a0. [DOI] [PubMed] [Google Scholar]
  13. Englund P. T., Deutscher M. P., Jovin T. M., Kelly R. B., Cozzarelli N. R., Kornberg A. Structural and functional properties of Escherichia coli DNA polymerase. Cold Spring Harb Symp Quant Biol. 1968;33:1–9. doi: 10.1101/sqb.1968.033.01.005. [DOI] [PubMed] [Google Scholar]
  14. Gass K. B., Cozzarelli N. R. Further genetic and enzymological characterization of the three Bacillus subtilis deoxyribonucleic acid polymerases. J Biol Chem. 1973 Nov 25;248(22):7688–7700. [PubMed] [Google Scholar]
  15. Goulian M. Some recent developments in DNA enzymology. Prog Nucleic Acid Res Mol Biol. 1972;12:29–48. doi: 10.1016/s0079-6603(08)60658-3. [DOI] [PubMed] [Google Scholar]
  16. Haines M. E., Holmes A. M., Johnston I. R. Distinct cytoplasmic and nuclear DNA polymerases from rat liver. FEBS Lett. 1971 Sep 15;17(1):63–67. doi: 10.1016/0014-5793(71)80564-1. [DOI] [PubMed] [Google Scholar]
  17. Haines M. E., Johnston I. R., Mathias A. P. The role of rat liver nuclear DNA polymerase and its distribution in various classes of liver nuclei. FEBS Lett. 1970 Sep 24;10(2):113–116. doi: 10.1016/0014-5793(70)80429-x. [DOI] [PubMed] [Google Scholar]
  18. Haines M. E., Wickremasinghe R. G., Johnston I. R. Purification and partial characterisation of rat-liver nuclear DNA polymerase. Eur J Biochem. 1972 Nov 21;31(1):119–129. doi: 10.1111/j.1432-1033.1972.tb02508.x. [DOI] [PubMed] [Google Scholar]
  19. Hamilton L., Mahler I., Grossman L. Enzymatic repair of deoxyribonucleic acid; the biochemical and biological repair properties of a deoxyribonucleic acid polymerase from micrococcus luteus. Biochemistry. 1974 Apr 23;13(9):1886–1896. doi: 10.1021/bi00706a017. [DOI] [PubMed] [Google Scholar]
  20. Hecht N. B. Enzymatically active intermediate in the conversion between the low and high molecular weight DNA polymerase. Nat New Biol. 1973 Oct 17;245(146):199–201. doi: 10.1038/newbio245199a0. [DOI] [PubMed] [Google Scholar]
  21. Hecht N. B. The relationship between two murine DNA-dependent DNA polymerases from the cytosol and the low molecular weight DNA polymerase. Biochim Biophys Acta. 1975 Apr 2;383(4):388–398. doi: 10.1016/0005-2787(75)90308-1. [DOI] [PubMed] [Google Scholar]
  22. Herrick G., Spear B. B., Veomett G. Intracellular localization of mouse DNA polymerase-alpha. Proc Natl Acad Sci U S A. 1976 Apr;73(4):1136–1139. doi: 10.1073/pnas.73.4.1136. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Holmer A. M., Hesslewood I. P., Johnston I. R. The occurrence of multiple activities in the high-molecular-weight DNA polymerase fraction of mammalian tissues. A preliminary study of some of their properties. Eur J Biochem. 1974 Apr 16;43(3):487–499. doi: 10.1111/j.1432-1033.1974.tb03436.x. [DOI] [PubMed] [Google Scholar]
  24. Holmes A. M., Johnston I. R. DNA polymerases of eukaryotes. FEBS Lett. 1975 Dec 15;60(2):233–243. doi: 10.1016/0014-5793(75)80721-6. [DOI] [PubMed] [Google Scholar]
  25. Holmes A. M., Johnston I. R. Molecular asymmetry of rat liver cytoplasmic DNA polymerase. FEBS Lett. 1973 Jan 1;29(1):1–6. doi: 10.1016/0014-5793(73)80002-x. [DOI] [PubMed] [Google Scholar]
  26. Loomis L. W., Rossomando E. F., Chang L. M. DNA polymerase of Dictyostelium discoideum. Biochim Biophys Acta. 1976 Apr 2;425(4):469–477. doi: 10.1016/0005-2787(76)90011-3. [DOI] [PubMed] [Google Scholar]
  27. Matsukage A., Bohn E. W., Wilson S. H. Multiple forms of DNA polymerase in mouse myeloma. Proc Natl Acad Sci U S A. 1974 Feb;71(2):578–582. doi: 10.1073/pnas.71.2.578. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. McLennan A. G., Keir H. M. Deoxyribonucleic acid polymerases of Euglena gracilis. Primer-template utilization of and enzyme activities associated with the two deoxyribonucleic acid polymerases of high molecular weight. Biochem J. 1975 Nov;151(2):239–247. doi: 10.1042/bj1510239. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. McLennan A. G., Keir H. M. Deoxyribonucleic acid polymerases of Euglena gracilis. Purification and properties of two distinct deoxyribonucleic acid polymerases of high molecular weight. Biochem J. 1975 Nov;151(2):227–238. doi: 10.1042/bj1510227. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. McLennan A. G., Keir H. M. Subcellular location and growth stage dependence of the DNA polymerases of Euglena gracilis. Biochim Biophys Acta. 1975 Oct 15;407(3):253–262. doi: 10.1016/0005-2787(75)90092-1. [DOI] [PubMed] [Google Scholar]
  31. McLennan A. G., Keir H. M. The deoxyribonucleic acid polymerases of non-vertebrate eukaryotes. Biochem Soc Symp. 1977;(42):55–73. [PubMed] [Google Scholar]
  32. Morioka K., Terayama H. A conversion factor for cytoplasmic DNA polymerase of rat liver. Biochem Biophys Res Commun. 1974 Nov 27;61(2):568–575. doi: 10.1016/0006-291x(74)90995-4. [DOI] [PubMed] [Google Scholar]
  33. Nes I. F., Kleppe K. Multiple DNA polymerases in Acinetobacter calcoaceticus. FEBS Lett. 1974 Jul 15;43(2):180–184. doi: 10.1016/0014-5793(74)80995-6. [DOI] [PubMed] [Google Scholar]
  34. Nes I. F., Lillehaug J. R., Kleppe K. DNA polymerase I from Acinetobacter calcoaceticus: enzymatic properties. Eur J Biochem. 1974 Dec 16;50(1):167–175. doi: 10.1111/j.1432-1033.1974.tb03884.x. [DOI] [PubMed] [Google Scholar]
  35. OKAZAKI T., KORNBERG A. ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. XV. PURIFICATION AND PROPERTIES OF A POLYMERASE FROM BACILLUS SUBTILIS. J Biol Chem. 1964 Jan;239:259–268. [PubMed] [Google Scholar]
  36. Polman B. J., Janssen H. M., Kuyper C. M. Activities of DNA degrading enzymes in the slime mold Physarum polycephalum. Evidence for five different enzymes. Arch Mikrobiol. 1974 Mar 4;96(2):119–124. [PubMed] [Google Scholar]
  37. RICHARDSON C. C., SCHILDKRAUT C. L., APOSHIAN H. V., KORNBERG A. ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. XIV. FURTHER PURIFICATION AND PROPERTIES OF DEOXYRIBONUCLEIC ACID POLYMERASE OF ESCHERICHIA COLI. J Biol Chem. 1964 Jan;239:222–232. [PubMed] [Google Scholar]
  38. Ross C. A., Harris W. J. Deoxyribonucleic acid polymerase from Chlamydomonas reinhardii. Biochem Soc Trans. 1976;4(4):806–807. doi: 10.1042/bst0040806. [DOI] [PubMed] [Google Scholar]
  39. Roufa D. J., Moses R. E., Reed S. J. The DNA polymerases of Chinese hamster cells. Subcellular distribution and properties of two DNA polymerases. Arch Biochem Biophys. 1975 Apr;167(2):547–559. doi: 10.1016/0003-9861(75)90498-1. [DOI] [PubMed] [Google Scholar]
  40. Sedwick W. D., Wang T. S., Korn D. Purification and properties of nuclear and cytoplasmic deoxyribonucleic acid polymerases from human KB cells. J Biol Chem. 1972 Aug 25;247(16):5026–5033. [PubMed] [Google Scholar]
  41. Shepherd J. B., Keir H. M. Deoxyribonucleic acid nucleotidyltransferase from Landschütz ascites-tumour cells. Biochem J. 1966 May;99(2):443–453. doi: 10.1042/bj0990443. [DOI] [PMC free article] [PubMed] [Google Scholar]
  42. Sherman M. I., Kang H. S. DNA polymerases in midgestation mouse embryo, trophoblast, and decidua. Dev Biol. 1973 Oct;34(2):200–210. doi: 10.1016/0012-1606(73)90350-3. [DOI] [PubMed] [Google Scholar]
  43. Spadari S., Muller R., Weissbach A. The dissimilitude of the low and high molecular weight deoxyribonucleic acid-dependent deoxyribonucleic acid polymerases of HeLa cells. J Biol Chem. 1974 May 10;249(9):2991–2992. [PubMed] [Google Scholar]
  44. Tait A., Cummings D. J. DNA-dependent DNA polymerase activities from Paramecia macronuclei. Biochim Biophys Acta. 1975 Jan 20;378(2):282–295. doi: 10.1016/0005-2787(75)90116-1. [DOI] [PubMed] [Google Scholar]
  45. Talmud P. J., Lewis D. The mutagenicity of amino acid analogues in Coprinus lagopus. Genet Res. 1974 Feb;23(1):47–61. doi: 10.1017/s0016672300014658. [DOI] [PubMed] [Google Scholar]
  46. Talmud P. J. Mutational synergism between p-fluorophenylalanine and UV in Coprinus lagopus. Mutat Res. 1977 May;43(2):213–222. doi: 10.1016/0027-5107(77)90005-7. [DOI] [PubMed] [Google Scholar]
  47. Talmud P., Lewis D. Mutagenicity of amino acid analogues in eukaryotes. Nature. 1974 Jun 7;249(457):563–564. doi: 10.1038/249563a0. [DOI] [PubMed] [Google Scholar]
  48. Tsuruo T., Ukita T. Purification and further characterization of three DNA polymerases of rat Ascites hepatoma cells. Biochim Biophys Acta. 1974 Jun 27;353(2):146–159. doi: 10.1016/0005-2787(74)90181-6. [DOI] [PubMed] [Google Scholar]
  49. Weissbach A., Baltimore D., Bollum F., Gallo R., Korn D. Nomenclature of eukaryotic DNA polymerases. Science. 1975 Oct 24;190(4212):401–402. doi: 10.1126/science.1179222. [DOI] [PubMed] [Google Scholar]
  50. Wintersberger E. Deoxyribonucleic acid polymerases from yeast. Further purification and characterization of DNA-dependent DNA polymerases A and B. Eur J Biochem. 1974 Dec 16;50(1):41–47. doi: 10.1111/j.1432-1033.1974.tb03871.x. [DOI] [PubMed] [Google Scholar]
  51. Wintersberger U. Absence of a low-molecular-weight DNA polymerase from nuclei of the yeast, Saccharomyces cerevisiae. Eur J Biochem. 1974 Dec 16;50(1):197–202. doi: 10.1111/j.1432-1033.1974.tb03888.x. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES