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. 2024 Dec 6;12:e18637. doi: 10.7717/peerj.18637

Table 1. Comparison of collagen scaffolds and CMC-PEG gels.

Material Origin Preparation Sterilization Manipulation Washability Variability
Collagen scaffolds Natural (bovine collagen) Disintegration → freeze-drying → cross-linking → freeze-drying Ethylene oxide or gamma irradiation Tweezers Continuous experiments possible–scaffolds are washable and transferrable Variable–each material has slightly different pore distribution and orientation;
an unknown number of cells successfully seeded in the scaffolds (cell losses due to cells partly flowing outside the scaffold)
CMC-PEG gel CMC–natural;
PEG–synthetic
Powder + H2O → 120-140 °C for 30 min UV light Weighing, wide-bore tip pipetting Difficult to wash (all additives mostly remain in the gel) Each material displays the same features;
a known number of cells seeded into the gel

Note:

Washability refers to the ability of materials to be washed after exposure to a substance, which is non-toxic for a short period of time but can be toxic after long-exposure (e.g., AlamarBlue).