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. 1981 Jan 15;194(1):369–372. doi: 10.1042/bj1940369

Differential cytotoxicity of daunomycin in tumour cells is related to glutathione-dependent hydrogen peroxide metabolism

Argante Bozzi 1, Irene Mavelli 1, Bruno Mondovi 1, Roberto Strom 1, Giuseppe Rotilio 1
PMCID: PMC1162754  PMID: 7305990

Abstract

Addition of 0.5mm-daunomycin, a quinone anti-cancer drug, causes severe inhibition of respiration in Ehrlich ascites cells, whereas Yoshida ascites cells were almost as resistant as rat hepatocytes. An inverse relationship appears to exist in the two types of tumour cells (which are both catalase-deficient) between the extent of cellular damage brought about by intracellular formation of superoxide anion occurring on reaction with O2 of the drug free radical and the efficiency of the glutathione-mediated H2O2-detoxifying system.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bachur N. R., Gordon S. L., Gee M. V. A general mechanism for microsomal activation of quinone anticancer agents to free radicals. Cancer Res. 1978 Jun;38(6):1745–1750. [PubMed] [Google Scholar]
  2. Bozzi A., Mavelli I., Finazzi A., Strom R., Wolf A. M., Mondovi B., Rotilio G. Enzyme defense against reactive oxygen derivatives. II. Erythrocytes and tumor cells. Mol Cell Biochem. 1976 Jan 31;10(1):11–16. doi: 10.1007/BF01731676. [DOI] [PubMed] [Google Scholar]
  3. Bozzi A., Mavelli I., Mondovì B., Strom R., Rotilio G. Differential sensitivity of tumor cells to externally generated hydrogen peroxide. Role of glutathione and related enzymes. Cancer Biochem Biophys. 1979;3(3):135–141. [PubMed] [Google Scholar]
  4. Goodman J., Hochstein P. Generation of free radicals and lipid peroxidation by redox cycling of adriamycin and daunomycin. Biochem Biophys Res Commun. 1977 Jul 25;77(2):797–803. doi: 10.1016/s0006-291x(77)80048-x. [DOI] [PubMed] [Google Scholar]
  5. Handa K., Sato S. Generation of free radicals of quinone group-containing anti-cancer chemicals in NADPH-microsome system as evidenced by initiation of sulfite oxidation. Gan. 1975 Feb;66(1):43–47. [PubMed] [Google Scholar]
  6. Jones D. P., Thor H., Andersson B., Orrenius S. Detoxification reactions in isolated hepatocytes. Role of glutathione peroxidase, catalase, and formaldehyde dehydrogenase in reactions relating to N-demethylation by the cytochrome P-450 system. J Biol Chem. 1978 Sep 10;253(17):6031–6037. [PubMed] [Google Scholar]
  7. Misra H. P., Fridovich I. The univalent reduction of oxygen by reduced flavins and quinones. J Biol Chem. 1972 Jan 10;247(1):188–192. [PubMed] [Google Scholar]
  8. Moldéus P., Högberg J., Orrenius S. Isolation and use of liver cells. Methods Enzymol. 1978;52:60–71. doi: 10.1016/s0076-6879(78)52006-5. [DOI] [PubMed] [Google Scholar]
  9. Rotilio G., Calabrese L., Finazzi Agrò A., Argento-Cerù M. P., Autuori F., Mondovì B. Intracellular localization of superoxide dismutase and its relation to the distribution and mechanism of hydrogen peroxide-producting enzymes. Biochim Biophys Acta. 1973 Sep 15;321(1):98–102. doi: 10.1016/0005-2744(73)90063-6. [DOI] [PubMed] [Google Scholar]
  10. Strom R., Bozzi A., Santoro A. S., Crifò C., Mondovì B., Fanelli A. R. Studies on the mechanism of inhibition by polyenic antibiotics of nucleic acid biosynthesis in ascites tumor cells. Cancer Res. 1972 Apr;32(4):868–876. [PubMed] [Google Scholar]
  11. Thayer W. S. Adriamycin stimulated superoxide formation in submitochondrial particles. Chem Biol Interact. 1977 Dec;19(3):265–278. doi: 10.1016/0009-2797(77)90050-3. [DOI] [PubMed] [Google Scholar]

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