Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1981 Feb 15;194(2):443–449. doi: 10.1042/bj1940443

Cystine uptake by rat renal brush-border vesicles.

P D McNamara, L M Pepe, S Segal
PMCID: PMC1162767  PMID: 7306000

Abstract

Uptake of L-cystine by brush-border membrane vesicles isolated from rat renal-cortical tissue was time-dependent and occurred in the absence of cystine reduction. A significant capacity for vesicular binding of cystine was observed. The amount bound increased with time of incubation and could be displaced by thiol reagents. At early time points, cystine uptake measured the transport of cystine into the intravesicular space. Total cystine uptake was mediated by multiple transport systems, including a low-Km high-affinity component which was shared by lysine, arginine, ornithine and glutamine and on which hetero-exchange diffusion of lysine and cystine was demonstrated.

Full text

PDF
443

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Askelöf P., Axelsson K., Eriksson S., Mannervik B. Mechanism of action of enzymes catalyzing thiol-disulfide interchange. Thioltransferases rather than transhydrogenases. FEBS Lett. 1974 Jan 15;38(3):263–267. doi: 10.1016/0014-5793(74)80068-2. [DOI] [PubMed] [Google Scholar]
  2. Beck J. C., Sacktor B. The sodium electrochemical potential-mediated uphill transport of D-glucose in renal brush border membrane vesicles. J Biol Chem. 1978 Aug 10;253(15):5531–5535. [PubMed] [Google Scholar]
  3. Booth A. G., Kenny A. J. A rapid method for the preparation of microvilli from rabbit kidney. Biochem J. 1974 Sep;142(3):575–581. doi: 10.1042/bj1420575. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bovee K. C., Thier S. O., Rea C., Segal S. Renal clearance of amino acids in canine cystinuria. Metabolism. 1974 Jan;23(1):51–58. doi: 10.1016/0026-0495(74)90103-6. [DOI] [PubMed] [Google Scholar]
  5. Brodehl J., Gellissen K., Kowalewski S. Isolierter Defekt der tubulären Cystin-Rückresorption in einer Familie mit idiopathischem Hypoparathyroidismus. Klin Wochenschr. 1967 Jan 1;45(1):38–40. doi: 10.1007/BF01745737. [DOI] [PubMed] [Google Scholar]
  6. DENT C. E., ROSE G. A. Aminoacid metabolism in cystinuria. Q J Med. 1951 Jul;20(79):205–219. [PubMed] [Google Scholar]
  7. Evers J., Murer H., Kinne R. Phenylalanine uptake in isolated renal brush border vesicles. Biochim Biophys Acta. 1976 Apr 5;426(4):598–615. doi: 10.1016/0005-2736(76)90124-3. [DOI] [PubMed] [Google Scholar]
  8. FOX M., THIER S., ROSENBERG L., KISER W., SEGAL S. EVIDENCE AGAINST A SINGLE RENAL TRANSPORT DEFECT IN CYSTINURIA. N Engl J Med. 1964 Mar 12;270:556–561. doi: 10.1056/NEJM196403122701105. [DOI] [PubMed] [Google Scholar]
  9. Foreman J. W., Hwang S. M., Segal S. Transport interactions of cystine and dibasic amino acids in isolated rat renal tubules. Metabolism. 1980 Jan;29(1):53–61. doi: 10.1016/0026-0495(80)90098-0. [DOI] [PubMed] [Google Scholar]
  10. Gmaj P., Murer H., Kinne R. Calcium ion transport across plasma membranes isolated from rat kidney cortex. Biochem J. 1979 Mar 15;178(3):549–557. doi: 10.1042/bj1780549. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. McNamara P. D., Koldovsky O., Segal S. Synthesis and degradation of microvillar protein of kidney in adult rats. FEBS Lett. 1974 Apr 15;41(1):139–142. doi: 10.1016/0014-5793(74)80973-7. [DOI] [PubMed] [Google Scholar]
  12. McNamara P. D., Ozegović B., Pepe L. M., Segal S. Proline and glycine uptake by renal brushborder membrane vesicles. Proc Natl Acad Sci U S A. 1976 Dec;73(12):4521–4525. doi: 10.1073/pnas.73.12.4521. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. ROBSON E. B., ROSE G. A. The effect of intravenous lysine on the renal clearances of cystine, arginine and ornithine in normal subjects, in patients with cystinuria and Fanconi syndrome and in their relatives. Clin Sci. 1957 Feb;16(1):75–93. [PubMed] [Google Scholar]
  14. ROSENBERG L. E., DOWNING S. J., SEGAL S. Competitive inhibition of dibasic amino acid transport in rat kidney. J Biol Chem. 1962 Jul;237:2265–2270. [PubMed] [Google Scholar]
  15. Segal S., McNamara P. D., Pepe L. M. Transport interaction of cystine and dibasic amino acids in renal brush border vesicles. Science. 1977 Jul 8;197(4299):169–171. doi: 10.1126/science.877548. [DOI] [PubMed] [Google Scholar]
  16. Segal S., Smith I. Delineation of cystine and cysteine transport systems in rat kidney cortex by developmental patterns. Proc Natl Acad Sci U S A. 1969 Jul;63(3):926–933. doi: 10.1073/pnas.63.3.926. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. States B., Segal S. Thin-layer chromatographic separation of cystine and the N-ethylmaleimide adducts of cysteine and glutathionen. Anal Biochem. 1969 Feb;27(2):323–329. doi: 10.1016/0003-2697(69)90041-4. [DOI] [PubMed] [Google Scholar]
  18. Tietze F., Bradley K. H., Schulman J. D. Enzymic reduction of cystine by subcellular fractions of cultured and peripheral leukocytes from normal and cystinotic individuals. Pediatr Res. 1972 Aug;6(8):649–658. doi: 10.1203/00006450-197208000-00002. [DOI] [PubMed] [Google Scholar]
  19. WEBBER W. A., BROWN J. L., PITTS R. F. Interactions of amino acids in renal tubular transport. Am J Physiol. 1961 Feb;200:380–386. doi: 10.1152/ajplegacy.1961.200.2.380. [DOI] [PubMed] [Google Scholar]
  20. Whelan D. T., Scriver C. R. Hyperdibasicaminoaciduria: an inherited disorder of amino acid transport. Pediatr Res. 1968 Nov;2(6):525–534. doi: 10.1203/00006450-196811000-00011. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES