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. 1981 May 1;195(2):399–405. doi: 10.1042/bj1950399

Dianthins, ribosome-damaging proteins with anti-viral properties from Dianthus caryophyllus L. (carnation).

F Stirpe, D G Williams, L J Onyon, R F Legg, W A Stevens
PMCID: PMC1162903  PMID: 7316958

Abstract

1. Dianthin 30 and dianthin 32, two proteins isolated from the leaves of Diathus caryophyllus (carnation), were purified to homogeneity by chromatography on CM-cellulose. 2. The mol.wt. of dianthin 30 is 29 500 and that of dianthin 32 is 31 700. Both dianthins are glycoproteins containing mannose. 3. Dianthins inhibit protein synthesis in a lysate of rabbit reticulocytes, with an ID50 (concentration giving 50% inhibition) of 9.15 ng/ml (dianthin 30) and 3.6 ng/ml (dianthin 32). They act by damaging ribosomes in a less-than-equimolar ratio. Protein synthesis by intact cells is partially inhibited by dianthins at a concentration of 100 microgram/ml. 4. Dianthins mixed with tobacco-mosaic virus strongly decrease the number of local lesions on leaves of Nicotiana glutinosa.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALLEN E. H., SCHWEET R. S. Synthesis of hemoglobin in a cell-free system. I. Properties of the complete system. J Biol Chem. 1962 Mar;237:760–767. [PubMed] [Google Scholar]
  2. Andrews P. Estimation of molecular size and molecular weights of biological compounds by gel filtration. Methods Biochem Anal. 1970;18:1–53. [PubMed] [Google Scholar]
  3. Barbieri L., Lorenzoni E., Stirpe F. Inhibition of protein synthesis in vitro by a lectin from Momordica charantia and by other haemagglutinins. Biochem J. 1979 Aug 15;182(2):633–635. doi: 10.1042/bj1820633. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Barbieri L., Zamboni M., Lorenzoni E., Montanaro L., Sperti S., Stirpe F. Inhibition of protein synthesis in vitro by proteins from the seeds of Momordica charantia (bitter pear melon). Biochem J. 1980 Feb 15;186(2):443–452. doi: 10.1042/bj1860443. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Barbieri L., Zamboni M., Montanaro L., Sperti S., Stirpe F. Purification and properties of different forms of modeccin, the toxin of Adenia digitata. Separation of subunits with inhibitory and lectin activity. Biochem J. 1980 Jan 1;185(1):203–210. doi: 10.1042/bj1850203. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. FANTES K. H., O'NEILL C. F. SOME SIMILARITIES BETWEEN A VIRAL INHIBITOR OF PLANT ORIGIN AND CHICK INTERFERON. Nature. 1964 Sep 5;203:1048–1050. doi: 10.1038/2031048a0. [DOI] [PubMed] [Google Scholar]
  7. Gasperi-Campani A., Barbieri L., Lorenzoni E., Montanaro L., Sperti S., Bonetti E., Stirpe F. Modeccin, the toxin of Adenia digitata. Purification, toxicity and inhibition of protein synthesis in vitro. Biochem J. 1978 Aug 15;174(2):491–496. doi: 10.1042/bj1740491. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gasperi-Campani A., TBarbieri L., Lorenzoni E., Stirpe F. Inhibition of protein synthesis by seed-extracts. A screening study. FEBS Lett. 1977 Apr 15;76(2):173–176. doi: 10.1016/0014-5793(77)80145-2. [DOI] [PubMed] [Google Scholar]
  9. Gooding G. V., Jr, Hebert T. T. A simple technique for purification of tobacco mosaic virus in large quantities. Phytopathology. 1967 Nov;57(11):1285–1285. [PubMed] [Google Scholar]
  10. Irvin J. D., Kelly T., Robertus J. D. Purification and properties of a second antiviral protein from Phytolacca americana which inactivates eukaryotic ribosomes. Arch Biochem Biophys. 1980 Apr 1;200(2):418–425. doi: 10.1016/0003-9861(80)90372-0. [DOI] [PubMed] [Google Scholar]
  11. Irvin J. D. Purification and partial characterization of the antiviral protein from Phytolacca americana which inhibits eukaryotic protein synthesis. Arch Biochem Biophys. 1975 Aug;169(2):522–528. doi: 10.1016/0003-9861(75)90195-2. [DOI] [PubMed] [Google Scholar]
  12. Judah D. J., Legg R. F., Neal G. E. Development of resistance to cytotoxicity during aflatoxin carcinogenesis. Nature. 1977 Jan 27;265(5592):343–345. doi: 10.1038/265343b0. [DOI] [PubMed] [Google Scholar]
  13. Kalb V. F., Jr, Bernlohr R. W. A new spectrophotometric assay for protein in cell extracts. Anal Biochem. 1977 Oct;82(2):362–371. doi: 10.1016/0003-2697(77)90173-7. [DOI] [PubMed] [Google Scholar]
  14. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  15. Montanaro L., Sperti S., Zamboni M., Denaro M., Testoni G., Gasperi-Campani A., Stirpe F. Effect of modeccin on the steps of peptide-chain elongation. Biochem J. 1978 Nov 15;176(2):371–379. doi: 10.1042/bj1760371. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Nicolson G. L., Blaustein J., Etzler M. E. Characterization of two plant lectins from Ricinus communis and their quantitative interaction with a murine lymphoma. Biochemistry. 1974 Jan 1;13(1):196–204. doi: 10.1021/bi00698a029. [DOI] [PubMed] [Google Scholar]
  17. Nicolson G. L., Blaustein J. The interaction of Ricinus communis agglutinin with normal and tumor cell surfaces. Biochim Biophys Acta. 1972 May 9;266(2):543–547. doi: 10.1016/0005-2736(72)90109-5. [DOI] [PubMed] [Google Scholar]
  18. Obrig T. G., Irvin J. D., Hardesty B. The effect of an antiviral peptide on the ribosomal reactions of the peptide elongation enzymes, EF-I and EF-II. Arch Biochem Biophys. 1973 Apr;155(2):278–289. doi: 10.1016/0003-9861(73)90116-1. [DOI] [PubMed] [Google Scholar]
  19. Olsnes S., Haylett T., Refsnes K. Purification and characterization of the highly toxic lectin modeccin. J Biol Chem. 1978 Jul 25;253(14):5069–5073. [PubMed] [Google Scholar]
  20. Owens R. A., Bruening G., Shepherd R. J. A possible mechanism for the inhibition of plant viruses by a peptide from Phytolacca americana. Virology. 1973 Nov;56(1):390–393. doi: 10.1016/0042-6822(73)90319-x. [DOI] [PubMed] [Google Scholar]
  21. RAGETLI H. W., WEINTRAUB M. Purification and characteristics of a virus inhibitor from Dianthus caryophyllus L. I. Purification and activity. Virology. 1962 Oct;18:232–240. doi: 10.1016/0042-6822(62)90009-0. [DOI] [PubMed] [Google Scholar]
  22. RAGETLI H. W., WEINTRAUB M. Purification and characteristics of a virus inhibitor from Dianthus caryophyllus L. II. Characterization and mode of action. Virology. 1962 Oct;18:241–248. doi: 10.1016/0042-6822(62)90010-7. [DOI] [PubMed] [Google Scholar]
  23. Roberts W. K., Stewart T. S. Purification and properties of a translation inhibitor from wheat germ. Biochemistry. 1979 Jun 12;18(12):2615–2621. doi: 10.1021/bi00579a028. [DOI] [PubMed] [Google Scholar]
  24. Stirpe F., Legg R. F., Onyon L. J., Ziska P., Franz H. Inhibition of protein synthesis by a toxic lectin from Viscum album L. (mistletoe). Biochem J. 1980 Sep 15;190(3):843–845. doi: 10.1042/bj1900843. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Stirpe F., Olsnes S., Pihl A. Gelonin, a new inhibitor of protein synthesis, nontoxic to intact cells. Isolation, characterization, and preparation of cytotoxic complexes with concanavalin A. J Biol Chem. 1980 Jul 25;255(14):6947–6953. [PubMed] [Google Scholar]
  26. Uchida T., Mekada E., Okada Y. Hybrid toxin of the A chain of ricin toxin and a subunit of Wistaria floribunda lectin. Possible importance of the hydrophobic region for entry of toxin into the cell. J Biol Chem. 1980 Jul 25;255(14):6687–6693. [PubMed] [Google Scholar]
  27. VAN KAMMEN A., NOORDAM D., THUNG T. H. The mechanism of inhibition of infection with tobacco mosaic virus by an inhibitor from carnation sap. Virology. 1961 May;14:100–108. doi: 10.1016/0042-6822(61)90137-4. [DOI] [PubMed] [Google Scholar]

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