Abstract
Myelin was found to contain five major glycoproteins with molecular weights of 120000, 95000, 88000, 43000 and 38000. Light myelin contained only 5-7% of the amount of these glycoproteins in whole myelin, whereas heavy myelin and the membrane fraction contained amounts nearly identical with whole myelin. Since all the major and minor glycoproteins, with the exception of 120000-mol-wt. glycoprotein, were detected only after treating the myelin membrane with neuraminidase, N-acetylneuraminic acid is a terminal sugar residue in these glycoproteins.
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- Agrawal H. C., Banik N. L., Bone A. H., Davison A. N., Mitchell R. F., Spohn M. The identity of a myelin-like fraction isolated from developing brain. Biochem J. 1970 Dec;120(3):635–642. doi: 10.1042/bj1200635. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Agrawal H. C., Trotter J. L., Burton R. M., Mitchell R. F. Metabolic studies on myelin. Evidence for a precursor role of a myelin subfraction. Biochem J. 1974 Apr;140(1):99–109. doi: 10.1042/bj1400099. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Banik N. L., Davison A. N. Enzyme activity and composition of myelin and subcellular fractions in the developing rat brain. Biochem J. 1969 Dec;115(5):1051–1062. doi: 10.1042/bj1151051. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Benjamins J. A., Gray M., Morell P. Metabolic relationships between myelin subfractions: entry of proteins. J Neurochem. 1976 Aug;27(2):571–575. doi: 10.1111/j.1471-4159.1976.tb12284.x. [DOI] [PubMed] [Google Scholar]
- Benjamins J. A., Miller K., McKhann G. M. Myelin subfractions in developing rat brain: characterization and sulphatide metabolism. J Neurochem. 1973 Jun;20(6):1589–1603. doi: 10.1111/j.1471-4159.1973.tb00276.x. [DOI] [PubMed] [Google Scholar]
- Bonner W. M., Laskey R. A. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974 Jul 1;46(1):83–88. doi: 10.1111/j.1432-1033.1974.tb03599.x. [DOI] [PubMed] [Google Scholar]
- Chiu T. C., Babitch J. A. Topography of glycoproteins in the chick synaptosomal plasma membrane. Biochim Biophys Acta. 1978 Jun 16;510(1):112–123. doi: 10.1016/0005-2736(78)90134-7. [DOI] [PubMed] [Google Scholar]
- Fujimoto K., Roots B. I., Burton R. M., Agrawal H. C. Morphological and biochemical characterization of light and heavy myelin isolated from developing rat brain. Biochim Biophys Acta. 1976 Apr 5;426(4):659–668. doi: 10.1016/0005-2736(76)90130-9. [DOI] [PubMed] [Google Scholar]
- Gahmberg C. G., Hakomori S. Surface carbohydrates of hamster fibroblasts. II. Interaction of hamster NIL cell surfaces with Ricinus communis lectin and concanavalin A as revealed by surface galactosyl label. J Biol Chem. 1975 Apr 10;250(7):2447–2451. [PubMed] [Google Scholar]
- Gottlieb D. I., Merrell R., Glaser L. Temporal changes in embryonal cell surface recognition. Proc Natl Acad Sci U S A. 1974 May;71(5):1800–1802. doi: 10.1073/pnas.71.5.1800. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lane J. D., Fagg G. E. Protein and glycoprotein composition of myelin subfractions from the developing rat optic nerve and tract. J Neurochem. 1980 Jan;34(1):163–171. doi: 10.1111/j.1471-4159.1980.tb04635.x. [DOI] [PubMed] [Google Scholar]
- Matthieu J. M., Quarles R. H., Brady R. O., Webster H. de F. Variation of proteins, enzyme markers and gangliosides in myelin subfractions. Biochim Biophys Acta. 1973 Dec 5;329(2):305–317. doi: 10.1016/0304-4165(73)90295-x. [DOI] [PubMed] [Google Scholar]
- McIntyre L. J., Quarles R. H., Brady R. O. Lectin-binding proteins in central-nervous-system myelin. Detection of glycoproteins of purified myelin on polyacrylamide gels by [3h]concanavalin A binding. Biochem J. 1979 Nov 1;183(2):205–212. doi: 10.1042/bj1830205. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mena E. E., Hoeser C. A., Moore B. W. An improved method of preparing rat brain synaptic membranes. Elimination of a contaminating membrane containing 2',3'-cyclic nucleotide 3'-phosphohydrolase activity. Brain Res. 1980 Apr 21;188(1):207–231. doi: 10.1016/0006-8993(80)90569-7. [DOI] [PubMed] [Google Scholar]
- Poduslo J. F., Harman J. L., McFarlin D. E. Lectin receptors in central nervous system myelin. J Neurochem. 1980 Jun;34(6):1733–1744. doi: 10.1111/j.1471-4159.1980.tb11268.x. [DOI] [PubMed] [Google Scholar]
- Quarles R. H., Everly J. L., Brady R. O. Demonstration of a glycoprotein which is associated with a purified myelin fraction from rat brain. Biochem Biophys Res Commun. 1972 Apr 28;47(2):491–497. doi: 10.1016/0006-291x(72)90741-3. [DOI] [PubMed] [Google Scholar]
- Quarles R. H., Everly J. L., Brady R. O. Evidence for the close association of a glycoprotein with myelin in rat brain. J Neurochem. 1973 Nov;21(5):1177–1191. doi: 10.1111/j.1471-4159.1973.tb07573.x. [DOI] [PubMed] [Google Scholar]
- Quarles R. H., McIntyre L. J., Pasnak C. F. Lectin-binding proteins in central-nervous-system myelin. Binding of glycoproteins in purified myelin to immobilized lectins. Biochem J. 1979 Nov 1;183(2):213–221. doi: 10.1042/bj1830213. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reimerdes E. H., Klostermeyer H. Determination of proteolytic activities on casein substrates. Methods Enzymol. 1976;45:26–28. doi: 10.1016/s0076-6879(76)45005-x. [DOI] [PubMed] [Google Scholar]
- Steck T. L., Dawson G. Topographical distribution of complex carbohydrates in the erythrocyte membrane. J Biol Chem. 1974 Apr 10;249(7):2135–2142. [PubMed] [Google Scholar]
- Waehneldt T. V., Matthieu J. M., Neuhoff V. Characterization of a myelin-related fraction (SN 4) isolated from rat forebrain at two developmental stages. Brain Res. 1977 Dec 9;138(1):29–43. doi: 10.1016/0006-8993(77)90782-x. [DOI] [PubMed] [Google Scholar]
- Zanetta J. P., Sarlieve L. L., Mandel P., Vincendon G., Gombos G. Fractionation of glycoproteins associated to adult rat brain myelin fractions. J Neurochem. 1977 Nov;29(5):827–838. doi: 10.1111/j.1471-4159.1977.tb10725.x. [DOI] [PubMed] [Google Scholar]
- Zimmerman A. W., Quarles R. H., de Webster H., Matthieu J. M., Brady R. O. Characterization and protein analysis of myelin subfractions in rat brain: developmental and regional comparisons. J Neurochem. 1975 Dec;25(6):749–757. doi: 10.1111/j.1471-4159.1975.tb04404.x. [DOI] [PubMed] [Google Scholar]

