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. 1981 May 15;196(2):641–644. doi: 10.1042/bj1960641

Initiation of protein synthesis in cell-free extracts of Tetrahymena pyriformis.

K E Smith, E T David
PMCID: PMC1163041  PMID: 6797407

Abstract

Saturating concentrations of the initiation-specific inhibitors poly(I) and T-2 toxin inhibit protein synthesis by over 35% and cause ribosome 'run-off' from the polyribosomes. The elongation-specific inhibitor cycloheximide totally prevents protein synthesis and 'freezes' the ribosomes in the pattern of unincubated controls. These results prove that our Tetrahymena extracts are capable of protein-synthesis initiation, a conclusion which is confirmed by a 30% inhibition of synthesis by the mRNA cap analogue, 7-methylguanosine 5'-monophosphate.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allen N. E., Suyama Y. Protein synthesis in vitro with Tetrahymena mitochondrial ribosomes. Biochim Biophys Acta. 1972 Feb 15;259(3):369–377. doi: 10.1016/0005-2787(72)90311-5. [DOI] [PubMed] [Google Scholar]
  2. Canaani D., Revel M., Groner Y. Translational discrimination of 'capped' and 'non-capped' mRNAS: inhibition of a series of chemical analogs of m7GpppX. FEBS Lett. 1976 May 1;64(2):326–331. doi: 10.1016/0014-5793(76)80321-3. [DOI] [PubMed] [Google Scholar]
  3. Cannon M., Smith K. E., Carter C. J. Prevention, by ribosome-bound nascent polyphenylalanine chains, of the functional interaction of t-2 toxin with its receptor site. Biochem J. 1976 May 15;156(2):289–294. doi: 10.1042/bj1560289. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Conklin K. A., Chou S. C. Protein synthesis in a cell-free preparation from Tetrahymena pyriformis GL. Comp Biochem Physiol B. 1971 Dec 15;40(4):855–862. doi: 10.1016/0305-0491(71)90031-9. [DOI] [PubMed] [Google Scholar]
  5. David E. T., Smith K. E. Preparation and partial characterization of cell-free protein-synthesizing extracts from Tetrahymena pyriformis. Biochem J. 1981 Mar 15;194(3):761–770. doi: 10.1042/bj1940761. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hickey E. D., Weber L. A., Baglioni C. Inhibition of initiation of protein synthesis by 7-methylguanosine-5'-monophosphate. Proc Natl Acad Sci U S A. 1976 Jan;73(1):19–23. doi: 10.1073/pnas.73.1.19. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kaempfer R., Rosen H., Israeli R. Translational control: recognition of the methylated 5' end and an internal sequence in eukaryotic mRNA by the initiation factor that binds methionyl-tRNAfMet. Proc Natl Acad Sci U S A. 1978 Feb;75(2):650–654. doi: 10.1073/pnas.75.2.650. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Mager J., Lipmann F. AMINO ACID INCORPORATION AND THE REVERSION OF ITS INITIAL PHASE WITH CELL-FREE TETRAHYMENA PREPARATIONS. Proc Natl Acad Sci U S A. 1958 Apr;44(4):305–309. doi: 10.1073/pnas.44.4.305. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Pain V. M. Protein synthesis in a postmitochondrial supernatant system from rat liver. An effect of diabetes at the level of peptide chain initiation. FEBS Lett. 1973 Sep 1;35(1):169–172. doi: 10.1016/0014-5793(73)80603-9. [DOI] [PubMed] [Google Scholar]

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