Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1981 Jul 1;197(1):177–183. doi: 10.1042/bj1970177

The primary structure of the constant region of Basilea-rabbit immunoglobulin lambda-chains.

I Garcia, J C Jaton
PMCID: PMC1163068  PMID: 6797414

Abstract

The amino acid sequence of the constant region of the immunoglobulin lambda-chain of the rabbit (C lambda) has been determined. This chain was obtained from a homogeneous anti-(type II pneumococcal polysaccharide) antibody fraction raised in Basilea rabbit 4548. The C lambda-region sequence was established by the analysis of tryptic and some overlapping chymotryptic peptides and partly by homology with known C lambda-region sequences of lambda-chains from other species. Peptides were isolated by a combination of methods including high-voltage paper electrophoresis, gel filtration and high-pressure liquid chromatography. The peptides were subjected to automated Edman degradation in the presence of Polybrene. The sequence showed no evidence of heterogeneity. Large interspecies similarities, as well as amino acid interchanges, are apparent among various C lambda regions; the degrees of homology between rabbit C lambda region and the C lambda region from human, porcine and murine chains are 72.6, 71 and 72% respectively. The similarity of lambda-chains of different species is much greater than that found between rabbit kappa- and lambda-chains (about 34% homology). Genetic implications of these results are discussed.

Full text

PDF
177

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Appella E. Amino acid sequences of two mouse immunoglobulin lambda chains. Proc Natl Acad Sci U S A. 1971 Mar;68(3):590–594. doi: 10.1073/pnas.68.3.590. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Appella E., Chersi A., Rejnek J., Reisfeld R., Mage R. Rabbit immunoglobulin lambda chains: isolation and amino acid sequence of cysteine-containing peptides. Immunochemistry. 1974 Aug;11(8):395–402. doi: 10.1016/0019-2791(74)90072-x. [DOI] [PubMed] [Google Scholar]
  3. Appella E., Mage R. G., Dubiski S., Reisfeld R. A. Chemical and immunochemical evidence for different classes of rabbit light polypeptide chains. Proc Natl Acad Sci U S A. 1968 Jul;60(3):975–981. doi: 10.1073/pnas.60.3.975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Benammar A., Brézin C., Cazenave P. A. Rabbit immunoglobulin allotypy: a sixth allele at the b locus (b 95). Mol Immunol. 1979 Dec;16(12):983–987. doi: 10.1016/0161-5890(79)90031-2. [DOI] [PubMed] [Google Scholar]
  5. Benson J. R. Fluorescent peptide mapping with microgram quantities of protein. Anal Biochem. 1976 Apr;71(2):459–470. doi: 10.1016/s0003-2697(76)80012-7. [DOI] [PubMed] [Google Scholar]
  6. Bernard O., Hozumi N., Tonegawa S. Sequences of mouse immunoglobulin light chain genes before and after somatic changes. Cell. 1978 Dec;15(4):1133–1144. doi: 10.1016/0092-8674(78)90041-7. [DOI] [PubMed] [Google Scholar]
  7. Blaser K., Eisen H. N. Lambda2 light chains in normal mouse immunoglobulins. Proc Natl Acad Sci U S A. 1978 Mar;75(3):1495–1499. doi: 10.1073/pnas.75.3.1495. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Brack C., Hirama M., Lenhard-Schuller R., Tonegawa S. A complete immunoglobulin gene is created by somatic recombination. Cell. 1978 Sep;15(1):1–14. doi: 10.1016/0092-8674(78)90078-8. [DOI] [PubMed] [Google Scholar]
  9. Brandt D. C., Jaton J. C. Identical VL region sequences of two antibodies from two outbred rabbits exhibiting complete idiotypic cross-reactivity and probably the same antigen-binding site fine structure. J Immunol. 1978 Sep;121(3):1194–1198. [PubMed] [Google Scholar]
  10. Brandt D. C., Jaton J. C. Occurrence of idiotypically identical antibodies in the sera of two outbred rabbits hyperimmunized with type II pneumococcal vaccine. J Immunol. 1978 Sep;121(3):1188–1193. [PubMed] [Google Scholar]
  11. Brunhouse R. F., Cebra J. J. Guinea pig immunoglobulin light chain isotypes. I. Separation of kappa and lambda chains and the identification of three isotypes of the lambda chain constant homology region. Eur J Immunol. 1978 Dec;8(12):881–888. doi: 10.1002/eji.1830081211. [DOI] [PubMed] [Google Scholar]
  12. DRAY S. Effect of maternal isoantibodies on the quantitative expression of two allelic genes controlling gamma-globulin allotypic specificities. Nature. 1962 Aug 18;195:677–680. doi: 10.1038/195677a0. [DOI] [PubMed] [Google Scholar]
  13. DRAY S., YOUNG G. O., GERALD L. IMMUNOCHEMICAL IDENTIFICATION AND GENETICS OF RABBIT GAMMA-GLOBULIN ALLOTYPES. J Immunol. 1963 Sep;91:403–415. [PubMed] [Google Scholar]
  14. Dubiski S. Suppression of synthesis of allotypically defined immunoglobulins and compensation by another sub-class of immunoglobulin. Nature. 1967 Jun 24;214(5095):1365–1366. doi: 10.1038/2141365a0. [DOI] [PubMed] [Google Scholar]
  15. Dugan E. S., Bradshaw R. A., Simms E. S., Eisen H. N. Amino acid sequence of the light chain of a mouse myeloma protein (MOPC-315). Biochemistry. 1973 Dec 18;12(26):5400–5416. [PubMed] [Google Scholar]
  16. Ein D. Nonallelic behavior of the Oz groups in human lambda immunoglobulin chains. Proc Natl Acad Sci U S A. 1968 Jul;60(3):982–985. doi: 10.1073/pnas.60.3.982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Emorine L., Dutka S., Paroutaud P., Strosberg A. D. The structural correlates of the rabbit light chain b allotypes: sequence studies of b5 and b6 chains. Mol Immunol. 1979 Dec;16(12):997–1004. doi: 10.1016/0161-5890(79)90033-6. [DOI] [PubMed] [Google Scholar]
  18. Farnsworth V., Goodfliesh R., Rodkey S., Hood L. Immunoglobulin allotypes of rabbit kappa chains: polymorphism of a control mechanism regulating closely linked duplicated genes? Proc Natl Acad Sci U S A. 1976 Apr;73(4):1293–1296. doi: 10.1073/pnas.73.4.1293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Fullmer C. S., Wasserman R. H. Analytical peptide mapping by high performance liquid chromatography. Application to intestinal calcium-binding proteins. J Biol Chem. 1979 Aug 10;254(15):7208–7212. [PubMed] [Google Scholar]
  20. Garcia I., Jaton J. C. The immune response to type II and type VIII pneumococcal vaccines in Basilea rabbits lacking K light chains. Immunochemical and partial amino acid sequence studies of the light chains. Mol Immunol. 1979 Dec;16(12):1063–1071. doi: 10.1016/0161-5890(79)90040-3. [DOI] [PubMed] [Google Scholar]
  21. Gilman-Sachs A., Mage R. G., Young G. O., Alexander C., Dray S. Identification and genetic control of two rabbit immunoglobulin allotypes at a second light chain locus, the c locus. J Immunol. 1969 Dec;103(6):1159–1167. [PubMed] [Google Scholar]
  22. Good P. W., Notenboom R., Dubiski S., Cinader B. Basilea rabbit immunoglobulins: detection and characterization by specific alloantiserum. J Immunol. 1980 Sep;125(3):1293–1297. [PubMed] [Google Scholar]
  23. Hess M., Hilschmann N., Rivat L., Rivat C., Ropartz C. Isotypes in human immunoglobulin lambda-chains. Nat New Biol. 1971 Nov 10;234(45):58–61. doi: 10.1038/newbio234058a0. [DOI] [PubMed] [Google Scholar]
  24. Jaton J. C., Kelus A. S. Peptide mapping of the lambda-like chains of the BASILEA rabbits. Eur J Immunol. 1977 Feb;7(2):118–120. doi: 10.1002/eji.1830070212. [DOI] [PubMed] [Google Scholar]
  25. Kelus A. S., Weiss S. Variant strain of rabbits lacking immunoglobulin kappa polypeptide chain. Nature. 1977 Jan 13;265(5590):156–158. doi: 10.1038/265156a0. [DOI] [PubMed] [Google Scholar]
  26. Kindt T. J. Rabbit immunoglobulin allotypes: structure, immunology, and genetics. Adv Immunol. 1975;21:35–86. doi: 10.1016/s0065-2776(08)60218-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Mage R. G., Young G. O., Reisfeld R. A. The association of the c7 allotype of rabbits with some light polypeptide chains which lack b locus allotypy. J Immunol. 1968 Sep;101(3):617–620. [PubMed] [Google Scholar]
  28. Novotný J., Franek F. Different degrees of interspecies homology in immunoglobulin lamda chain constant domain correlated with three-dimensional structure. Nature. 1975 Dec 18;258(5536):641–643. doi: 10.1038/258641a0. [DOI] [PubMed] [Google Scholar]
  29. Novotný J., Franek F., Margolies M. N., Haber E. Amino acid sequence of normal (microheterogeneous) porcine immunoglobulin lambda chains. Biochemistry. 1977 Aug 23;16(17):3765–3772. doi: 10.1021/bi00636a006. [DOI] [PubMed] [Google Scholar]
  30. O'Donnell I. J., Frangione B., Porter R. R. The disulphide bonds of the heavy chain of rabbit immunoglobulin G. Biochem J. 1970 Jan;116(2):261–268. doi: 10.1042/bj1160261. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Poljak R. J., Amzel L. M., Avey H. P., Chen B. L., Phizackerley R. P., Saul F. Three-dimensional structure of the Fab' fragment of a human immunoglobulin at 2,8-A resolution. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3305–3310. doi: 10.1073/pnas.70.12.3305. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Rangarajan M., Darbre A. Identification of amino acid thiohydantoins directly by thin-layer chromatography and indirectly by gas-liquid chromatography after hydrolysis. Biochem J. 1975 Jun;147(3):435–438. doi: 10.1042/bj1470435. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Schiffer M., Girling R. L., Ely K. R., Edmundson A. B. Structure of a lambda-type Bence-Jones protein at 3.5-A resolution. Biochemistry. 1973 Nov 6;12(23):4620–4631. doi: 10.1021/bi00747a013. [DOI] [PubMed] [Google Scholar]
  34. Sogn J. A., Kindt T. J. A genetic polymorphism in the constant region of rabbit b4 kappa chains. J Exp Med. 1976 Jun 1;143(6):1475–1482. doi: 10.1084/jem.143.6.1475. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Tarr G. E., Beecher J. F., Bell M., McKean D. J. Polyquarternary amines prevent peptide loss from sequenators. Anal Biochem. 1978 Feb;84(2):622–7?0=ENG. doi: 10.1016/0003-2697(78)90086-6. [DOI] [PubMed] [Google Scholar]
  36. Weiss S., Kelus A. S., Braun D. G. Antibody response to the streptococcal group A-variant polysaccharide in BASILEA rabbits lacking kappa-polypeptide chains. J Exp Med. 1977 Nov 1;146(5):1195–1205. doi: 10.1084/jem.146.5.1195. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Wikler M., Titani K., Shinoda T., Putnam F. W. The complete amino acid sequence of a lambda type Bence-Jones protein. J Biol Chem. 1967 Apr 10;242(7):1668–1670. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES