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. 1981 Jun 15;196(3):669–673. doi: 10.1042/bj1960669

Characterization of brush borders purified in iso-osmotic medium and microvillar membranes subfractionated from mouse small intestine.

M Fujita, H Ohta, T Uezato
PMCID: PMC1163084  PMID: 7317008

Abstract

Brush borders free of nuclei were isolated by repeated homogenization and centrifugation in iso-osmotic medium. They showed typical morphology under electron microscopy. The mean recovery and enrichment of alkaline phosphatase activity in the brush-border fraction were 50% and 17.5-fold respectively. gamma-Glutamyl transpeptidase showed a close parallelism with alkaline phosphatase and sucrase in subcellular distribution. Microvillar membranes were purified from isolated brush borders; they showed a further enrichment for alkaline phosphatase and were composed of homogeneous vesicles. Both brush-border and microvillar-membrane preparations were analysed for contamination by basolateral and endoplasmic-reticular membranes. Sodium dodecyl sulphate/polyacrylamide-gel electrophoresis of the microvillar-membrane preparation in six different systems revealed approx. 40 components in the mol.wt. range 15 000-232 000. They were grouped into seven major classes on the basis of molecular weight and electrophoretic patterns.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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