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. 1981 Aug 1;197(2):327–332. doi: 10.1042/bj1970327

Cell-free biosynthesis of erythroglycan in a microsomal fraction from K-562 cells.

T Z Russin, R A Laine, S J Turco
PMCID: PMC1163131  PMID: 6798962

Abstract

Particulate membrane preparations from K-562 [human CML (chronic-myelogenous-leukaemia)-derived] cells catalyse the transfer of [3H]galactose from UDP-[3H]-galactose and [3H]N-acetylglucosamine from UDP-[3H]N-acetylglucosamine into an endogenous product that on digestion with Pronase yields long-chain glycopeptides (mol.wt. 7000--10 000) called 'erythroglycan'. Incorporation of either labelled sugar increased up to 60 min of incubation time. The labelled erythroglycan was isolated by chromatography on Sephadex G-50 and characterized by digestion with endo-beta-galactosidase from Escherichia freundii, followed by analysis on Bio-Gel P-2 and paper chromatography. This digestion gave the following four products: (1) a disaccharide with the sequence beta GlcNAc-beta Gal; (2) a trisaccharide with the sequence betaGal-betaGlcNAc-beta Gal; (3) a larger oligosaccharide containing galactose and N-acetylglucosamine; and (4) a putative protein-linkage region.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CHAPPELL J. B., GREVILLE G. D. Effect of silver ions on mitochondrial adenosine triphosphatase. Nature. 1954 Nov 13;174(4437):930–931. doi: 10.1038/174930b0. [DOI] [PubMed] [Google Scholar]
  2. Drickamer L. K. Orientation of the band 3 polypeptide from human erythrocyte membranes. Identification of NH2-terminal sequence and site of carbohydrate attachment. J Biol Chem. 1978 Oct 25;253(20):7242–7248. [PubMed] [Google Scholar]
  3. Fukuda M., Fukuda M. N., Hakomori S. Developmental change and genetic defect in the carbohydrate structure of band 3 glycoprotein of human erythrocyte membrane. J Biol Chem. 1979 May 25;254(10):3700–3703. [PubMed] [Google Scholar]
  4. Gahmberg C. G., Myllyla G., Leikola J., Pirkola A., Nordling S. Absence of the major sialoglycoprotein in the membrane of human En(a--) erythrocytes and increased glycosylation of band 3. J Biol Chem. 1976 Oct 10;251(19):6108–6116. [PubMed] [Google Scholar]
  5. Järnefelt J., Rush J., Li Y. T., Laine R. A. Erythroglycan, a high molecular weight glycopeptide with the repeating structure [galactosyl-(1 leads to 4)-2-deoxy-2-acetamido-glucosyl(1 leads to 3)] comprising more than one-third of the protein-bound carbohydrate of human erythrocyte stroma. J Biol Chem. 1978 Nov 25;253(22):8006–8009. [PubMed] [Google Scholar]
  6. Kornfeld R., Kornfeld S. Comparative aspects of glycoprotein structure. Annu Rev Biochem. 1976;45:217–237. doi: 10.1146/annurev.bi.45.070176.001245. [DOI] [PubMed] [Google Scholar]
  7. Krusius T., Finne J., Rauvala H. The poly(glycosyl) chains of glycoproteins. Characterisation of a novel type of glycoprotein saccharides from human erythrocyte membrane. Eur J Biochem. 1978 Dec 1;92(1):289–300. doi: 10.1111/j.1432-1033.1978.tb12747.x. [DOI] [PubMed] [Google Scholar]
  8. Li E., Gibson R., Kornfeld S. Structure of an unusual complex-type oligosaccharide isolated from Chinese hamster ovary cells. Arch Biochem Biophys. 1980 Feb;199(2):393–399. doi: 10.1016/0003-9861(80)90295-7. [DOI] [PubMed] [Google Scholar]
  9. Livingston D. M., Hinkle D. C., Richardson C. C. Deoxyribonucleic acid polymerase III of Escherichia coli. Purification and properties. J Biol Chem. 1975 Jan 25;250(2):461–469. [PubMed] [Google Scholar]
  10. Parodi A. J., Leloir L. F. The role of lipid intermediates in the glycosylation of proteins in the eucaryotic cell. Biochim Biophys Acta. 1979 Apr 23;559(1):1–37. doi: 10.1016/0304-4157(79)90006-6. [DOI] [PubMed] [Google Scholar]
  11. Robbins P. W., Krag S. S., Liu T. Effects of UDP-glucose addition on the synthesis of mannosyl lipid-linked oligosaccharides by cell-free fibroblast preparations. J Biol Chem. 1977 Mar 10;252(5):1780–1785. [PubMed] [Google Scholar]
  12. Rush J. S., Turco S. J., Laine R. A. Erythroglycan biosynthesis in K-562 cells. Inhibition of synthesis by tunicamycin and lack of attachment to the G-protein of vesicular-stomatitis virus. Biochem J. 1981 Jan 1;193(1):361–365. doi: 10.1042/bj1930361. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Tanner M. J., Boxer D. H. Separation and some properties of the major proteins of the human erythrocyte membrane. Biochem J. 1972 Sep;129(2):333–347. doi: 10.1042/bj1290333. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Turco S. J., Rush J. S., Laine R. A. Presence of erythroglycan on human K-562 chronic myelogenous leukemia-derived cells. J Biol Chem. 1980 Apr 25;255(8):3266–3269. [PubMed] [Google Scholar]
  15. Turco S. J., Stetson B., Robbins P. W. Comparative rates of transfer of lipid-linked oligosaccharides to endogenous glycoprotein acceptors in vitro. Proc Natl Acad Sci U S A. 1977 Oct;74(10):4411–4414. doi: 10.1073/pnas.74.10.4411. [DOI] [PMC free article] [PubMed] [Google Scholar]

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