Abstract
Borate and aminophenylboronic acid were tested as inhibitors of activation of inactive dinitrogenase reductase from Rhodospirillum rubrum. Inhibition was specific for activation because activity of the active form of the enzyme was not inhibited. Inhibition showed the pH-dependence expected if borate inhibits by binding to cis-hydroxy groups of the modifying group found on the inactive enzyme.
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Selected References
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- Annamalai A. E., Pal P. K., Colman R. F. Purification of nucleosidyl peptides by chromatography on dihydroxyboryl-substituted polyacrylamide and cellulose. Anal Biochem. 1979 Oct 15;99(1):85–91. doi: 10.1016/0003-2697(79)90046-0. [DOI] [PubMed] [Google Scholar]
- Carithers R. P., Yoch D. C., Arnon D. I. Two forms of nitrogenase from the photosynthetic bacterium Rhodospirillum rubrum. J Bacteriol. 1979 Feb;137(2):779–789. doi: 10.1128/jb.137.2.779-789.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Koch B., Evans H. J. Reduction of acetylene to ethylene by soybean root nodules. Plant Physiol. 1966 Dec;41(10):1748–1750. doi: 10.1104/pp.41.10.1748. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ludden P. W., Burris R. H. Activating factor for the iron protein of nitrogenase from Rhodospirillum rubrum. Science. 1976 Oct 22;194(4263):424–426. doi: 10.1126/science.824729. [DOI] [PubMed] [Google Scholar]
- Ludden P. W., Burris R. H. Purification and properties of nitrogenase from Rhodospirillum rubrum, and evidence for phosphate, ribose and an adenine-like unit covalently bound to the iron protein. Biochem J. 1978 Oct 1;175(1):251–259. doi: 10.1042/bj1750251. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ludden P. W., Burris R. H. Removal of an adenine-like molecule during activation of dinitrogenase reductase from Rhodospirillum rubrum. Proc Natl Acad Sci U S A. 1979 Dec;76(12):6201–6205. doi: 10.1073/pnas.76.12.6201. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nordlund S., Eriksson U., Baltscheffsky H. Necessity of a membrane component for nitrogenase activity in Rhodospirillum rubrum. Biochim Biophys Acta. 1977 Oct 12;462(1):187–195. doi: 10.1016/0005-2728(77)90201-8. [DOI] [PubMed] [Google Scholar]
- Van Ness B. G., Howard J. B., Bodley J. W. Isolation and properties of the trypsin-derived ADP-ribosyl peptide from diphtheria toxin-modified yeast elongation factor 2. J Biol Chem. 1978 Dec 25;253(24):8687–8690. [PubMed] [Google Scholar]
- Walker G. A., Mortenson L. E. An effect of magnesium adenosine 5'-triphosphate on the structure of azoferredoxin from Clostridium pasteurianum. Biochem Biophys Res Commun. 1973 Aug 6;53(3):904–909. doi: 10.1016/0006-291x(73)90177-0. [DOI] [PubMed] [Google Scholar]