Abstract
A D-galactose-binding lectin agglutinating human erythrocytes has been purified from the seeds of the Indian coral tree (Erythrina variegata (Linn.) var. orientalis (Linn.) Merrill] by affinity chromatography on acid-treated Sepharose-6B gel. It has a higher reactivity for O-group erythrocytes. The lectin is a glycoprotein having a leucoagglutinating property.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Brown J. C., Hunt R. C. Lectins. Int Rev Cytol. 1978;52:277–349. doi: 10.1016/s0074-7696(08)60758-5. [DOI] [PubMed] [Google Scholar]
- Chrambach A., Reisfeld R. A., Wyckoff M., Zaccari J. A procedure for rapid and sensitive staining of protein fractionated by polyacrylamide gel electrophoresis. Anal Biochem. 1967 Jul;20(1):150–154. doi: 10.1016/0003-2697(67)90272-2. [DOI] [PubMed] [Google Scholar]
- Ersson B., Aspberg K., Porath J. The phytohemagglutinin from sunn hemp seeds (Crotalaria juncea). Purification by biospecific affinity chromatography. Biochim Biophys Acta. 1973 Jun 15;310(2):446–452. doi: 10.1016/0005-2795(73)90128-1. [DOI] [PubMed] [Google Scholar]
- Goldstein I. J., Hayes C. E. The lectins: carbohydrate-binding proteins of plants and animals. Adv Carbohydr Chem Biochem. 1978;35:127–340. doi: 10.1016/s0065-2318(08)60220-6. [DOI] [PubMed] [Google Scholar]
- Horejsí V., Tichá M., Novotný J., Kocourek J. Studies on lectins. XLVII. Some properties of D-galactose binding lectins isolated from the seeds of Butea frondosa, Erythrina indica and Momordica charantia. Biochim Biophys Acta. 1980 Jun 26;623(2):439–448. doi: 10.1016/0005-2795(80)90273-1. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Lee D. W., Tan G. S., Liew F. Y. A survey of lectins in Southeast Asian leguminosae. Planta Med. 1977 Feb;31(1):83–93. doi: 10.1055/s-0028-1097497. [DOI] [PubMed] [Google Scholar]