Abstract
Ferritin was purified from the serum of two patients with idiopathic haemochromatosis. The protein contained three types of subunit--the H and L subunits of tissue ferritins (although only a trace of H could be detected) and a third subunit, 'G', with the highest apparent molecular weight. Only the 'G' subunit band stained for carbohydrate, indicating that a proportion of the subunits of human serum ferritin are glycosylated. Although serum was obtained from patients with idiopathic haemochromatosis, it is probable that the 'G' subunit is a component of normal serum ferritin.
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- Arosio P., Adelman T. G., Drysdale J. W. On ferritin heterogeneity. Further evidence for heteropolymers. J Biol Chem. 1978 Jun 25;253(12):4451–4458. [PubMed] [Google Scholar]
- Arosio P., Yokota M., Drysdale J. W. Characterization of serum ferritin in iron overload: possible identity to natural apoferritin. Br J Haematol. 1977 Jun;36(2):199–207. doi: 10.1111/j.1365-2141.1977.tb00640.x. [DOI] [PubMed] [Google Scholar]
- Cragg S. J., Wagstaff M., Worwood M. Sialic acid and the microheterogeneity of human serum ferritin. Clin Sci (Lond) 1980 Mar;58(3):259–262. doi: 10.1042/cs0580259. [DOI] [PubMed] [Google Scholar]
- Furlan M., Perret B. A., Beck E. A. Staining of glycoproteins in polyacrylamide and agarose gels with fluorescent lectins. Anal Biochem. 1979 Jul 1;96(1):208–214. doi: 10.1016/0003-2697(79)90574-8. [DOI] [PubMed] [Google Scholar]
- Görg A., Postel W., Westermeier R. Ultrathin-layer isoelectric focusing in polyacrylamide gels on cellophane. Anal Biochem. 1978 Aug 15;89(1):60–70. doi: 10.1016/0003-2697(78)90726-1. [DOI] [PubMed] [Google Scholar]
- Jones B. M., Worwood M. An immunoradiometric assay for the acidic ferritin of human heart: application to human tissues, cells and serum. Clin Chim Acta. 1978 Apr 3;85(1):81–88. doi: 10.1016/0009-8981(78)90104-3. [DOI] [PubMed] [Google Scholar]
- O'Farrell P. H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed] [Google Scholar]
- Puro D. G., Richter G. W. Ferritin synthesis by free and membrane-bound (poly)ribosomes of rat liver. Proc Soc Exp Biol Med. 1971 Nov;138(2):399–403. doi: 10.3181/00379727-138-35906. [DOI] [PubMed] [Google Scholar]
- Racusen D. Glycoprotein detection in polyacrylamide gel with thymol and sulfuric acid. Anal Biochem. 1979 Nov 1;99(2):474–476. doi: 10.1016/s0003-2697(79)80035-4. [DOI] [PubMed] [Google Scholar]
- Ryan S., Watson L. R., Tavassoli M., Green R., Crosby W. H. Methods for establishing a working immunoradiometric assay for serum ferritin. Am J Hematol. 1978;4(4):375–386. doi: 10.1002/ajh.2830040409. [DOI] [PubMed] [Google Scholar]
- Wagstaff M., Worwood M., Jacobs A. Properties of human tissue isoferritins. Biochem J. 1978 Sep 1;173(3):969–977. doi: 10.1042/bj1730969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Worwood M., Aherne W., Dawkins S., Jacobs A. The characteristics of ferritin from human tissues, serum and blood cells. Clin Sci Mol Med. 1975 May;48(5):441–451. doi: 10.1042/cs0480441. [DOI] [PubMed] [Google Scholar]
- Worwood M., Cragg S. J., Wagstaff M., Jacobs A. Binding of human serum ferritin to concanavalin A. Clin Sci (Lond) 1979 Jan;56(1):83–87. doi: 10.1042/cs0560083. [DOI] [PubMed] [Google Scholar]
- Worwood M., Dawkins S., Wagstaff M., Jacobs A. The purification and properties of ferritin from human serum. Biochem J. 1976 Jul 1;157(1):97–103. doi: 10.1042/bj1570097. [DOI] [PMC free article] [PubMed] [Google Scholar]