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. 2024 Nov 27;636(8042):499–508. doi: 10.1038/s41586-024-08263-6

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 2 — a, Representative cryo-electron micrograph of the human MCM loading reaction. b, 2D averages of nucleosomes, MCM, hMO* and hDH were observed in the dataset. Box size 432 Å. c, Processing pipeline for the hDH. d, Angular distribution of the hDH structure. e, Fourier shell correlation plot for the hDH structure. f, Three rotated views and one cut-through view of the hDH structure, color-coded according to the local resolution as estimated in cryoSPARC.