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. 1981 Nov 15;200(2):373–377. doi: 10.1042/bj2000373

The incorporation in vitro of sulphate ions into synaptic-membrane glycoproteins.

C J White
PMCID: PMC1163545  PMID: 7340836

Abstract

Synaptosomes from sheep brain cortex were incubated with carrier-free Na235SO4 and the synaptic plasma membranes were isolated. The membranes were free of contamination from cytosol, mitochondria and microsomal material and accounted for 30% of the radioactivity present in the synaptosomal particulate fraction. Control experiments demonstrated that the radioactivity present in the preparation was not due to non-specific binding of sulphate ions. The synaptic membranes contained at least six 35S-containing protein bands with molecular weights between 160 000 and 16 000. Analysis showed that the radioactivity was located in the carbohydrate moiety of a glycopeptide.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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