Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1981 Nov 15;200(2):435–440. doi: 10.1042/bj2000435

[3H]proline incorporation and hydroxyproline concentration in articular cartilage during the development of osteoarthritis caused by immobilization. A study in vivo with rabbits.

T Videman, I Eronen, T Candolin
PMCID: PMC1163554  PMID: 7340843

Abstract

Proline metabolism in vivo was studied during the development of immobilization osteoarthritis in rabbits. Collagen content was measured as the hydroxyproline concentration of the tissue in question. The incorporation of [3H]proline was used as the indicator for total protein synthesis; collagen synthesis rate was estimated from measurements of the specific radioactivity of hydroxyproline. Cartilage samples from knee and hip joints were analysed after 3, 7, 11, 18, 35 and 56 days of immobilization. The total protein and collagen synthesis rates of the immobilized legs increased and reached a maximum after 11-35 days. Although they decreased thereafter, these rates remained elevated to the end of the experiment. A slight increase in the synthetic activity of the non-immobilized contralateral legs was also detected after 7--18 days of immobilization. The isotope incorporation was markedly higher in tibial marginal tissue than in weight-bearing cartilage. In spite of the increased synthesis, no clear changes were found in the collagen content of the tissues studied during the experiment.

Full text

PDF
435

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BOLLET A. J., HANDY J. R., STURGILL B. C. Chondroitin sulfate concentration and protein-polysaccharide composition of articular cartilage in osteoarthritis. J Clin Invest. 1963 Jun;42:853–859. doi: 10.1172/JCI104777. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Candolin T., Videman T. Surface changes in the articular cartilage of rabbit knee during immobilization. A scanning electron microscopic study of experimental osteoarthritis. Acta Pathol Microbiol Scand A. 1980 Sep;88(5):291–297. doi: 10.1111/j.1699-0463.1980.tb02499.x. [DOI] [PubMed] [Google Scholar]
  3. Eronen I., Videman T., Friman C., Michelsson J. E. Glycosaminoglycan metabolism in experimental osteoarthrosis caused by immobilization. Acta Orthop Scand. 1978 Aug;49(4):329–334. doi: 10.3109/17453677809050083. [DOI] [PubMed] [Google Scholar]
  4. Eyre D. R., McDevitt C. A., Billingham M. E., Muir H. Biosynthesis of collagen and other matrix proteins by articular cartilage in experimental osteoarthrosis. Biochem J. 1980 Jun 15;188(3):823–837. doi: 10.1042/bj1880823. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Floman Y., Eyre D. R., Glimcher M. J. Induction of osteoarthrosis in the rabbit knee joint: biochemical studies on the articular cartilage. Clin Orthop Relat Res. 1980 Mar-Apr;(147):278–286. [PubMed] [Google Scholar]
  6. Gardner D. L. The Experimental Production of Arthritis: A Review. Ann Rheum Dis. 1960 Dec;19(4):297–317. doi: 10.1136/ard.19.4.297. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Juva K., Prockop D. J. Modified procedure for the assay of H-3-or C-14-labeled hydroxyproline. Anal Biochem. 1966 Apr;15(1):77–83. doi: 10.1016/0003-2697(66)90249-1. [DOI] [PubMed] [Google Scholar]
  8. Kivirikko K. I., Laitinen O., Prockop D. J. Modifications of a specific assay for hydroxyproline in urine. Anal Biochem. 1967 May;19(2):249–255. doi: 10.1016/0003-2697(67)90160-1. [DOI] [PubMed] [Google Scholar]
  9. Langenskiöld A., Michelsson J. E., Videman T. Osteoarthritis of the knee in the rabbit produced by immobilization. Attempts to achieve a reproducible model for studies on pathogenesis and therapy. Acta Orthop Scand. 1979 Feb;50(1):1–14. doi: 10.3109/17453677909024083. [DOI] [PubMed] [Google Scholar]
  10. Lippiello L., Hall D., Mankin H. J. Collagen synthesis in normal and osteoarthritic human cartilage. J Clin Invest. 1977 Apr;59(4):593–600. doi: 10.1172/JCI108676. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mankin H. J., Lippiello L. Biochemical and metabolic abnormalities in articular cartilage from osteo-arthritic human hips. J Bone Joint Surg Am. 1970 Apr;52(3):424–434. [PubMed] [Google Scholar]
  12. Mankin H. J., Lippiello L. The glycosaminoglycans of normal and arthritic cartilage. J Clin Invest. 1971 Aug;50(8):1712–1719. doi: 10.1172/JCI106660. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Stegemann H., Stalder K. Determination of hydroxyproline. Clin Chim Acta. 1967 Nov;18(2):267–273. doi: 10.1016/0009-8981(67)90167-2. [DOI] [PubMed] [Google Scholar]
  14. Videman T., Eronen I., Friman C. Glycosaminoglycan metabolism in experimental osteoarthritis caused by immobilization. The effects of different periods of immobilization and follow-up. Acta Orthop Scand. 1981 Feb;52(1):11–21. doi: 10.3109/17453678108991751. [DOI] [PubMed] [Google Scholar]
  15. Videman T., Eronen I., Friman C., Langenskiöld A. Glycosaminoglycan metabolism of the medial meniscus, the medial collateral ligament and the hip joint capsule in experimental osteoarthritis caused by immobilization of the rabbit knee. Acta Orthop Scand. 1979 Aug;50(4):465–470. doi: 10.3109/17453677908989791. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES