Abstract
The salt dependence of the iron-binding properties of transferrin was studied by urea/polyacrylamide-gel electrophoresis. The distribution of iron between the N-terminal and C-terminal binding sites under equilibrium conditions and the rates of release of iron from the two sites were studied. It was found that salt increases the thermodynamic stability of iron binding in the N-terminal site relative to the C-terminal site. Similar behaviour is observed for the kinetics of iron release, where salt retards the rate of removal of iron from the N-terminal site but facilitates removal from the C-terminal site.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Baldwin D. A. The kinetics of iron release from human transferrin by EDTA. Effect of salts and detergents. Biochim Biophys Acta. 1980 May 29;623(1):183–198. doi: 10.1016/0005-2795(80)90020-3. [DOI] [PubMed] [Google Scholar]
- Baldwin D. A., de Sousa D. M. The effect of salts on the kinetics of iron release from N-terminal and C terminal monoferrictransferrins. Biochem Biophys Res Commun. 1981 Apr 30;99(4):1101–1107. doi: 10.1016/0006-291x(81)90732-4. [DOI] [PubMed] [Google Scholar]
- Chasteen N. D., Williams J. The influence of pH on the equilibrium distribution of iron between the metal-binding sites of human transferrin. Biochem J. 1981 Mar 1;193(3):717–727. doi: 10.1042/bj1930717. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Morgan E. H., Huebers H., Finch C. A. Differences between the binding sites for iron binding and release in human and rat transferrin. Blood. 1978 Dec;52(6):1219–1228. [PubMed] [Google Scholar]
- Morgan E. H. Studies on the mechanism of iron release from transferrin. Biochim Biophys Acta. 1979 Oct 24;580(2):312–326. doi: 10.1016/0005-2795(79)90144-2. [DOI] [PubMed] [Google Scholar]
- Price E. M., Gibson J. F. Electron paramagnetic resonance evidence for a distinction between the two iron-binding sites in transferrin and in conalbumin. J Biol Chem. 1972 Dec 25;247(24):8031–8035. [PubMed] [Google Scholar]
- Williams J., Moreton K. The distribution of iron between the metal-binding sites of transferrin human serum. Biochem J. 1980 Feb 1;185(2):483–488. doi: 10.1042/bj1850483. [DOI] [PMC free article] [PubMed] [Google Scholar]