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. 1976 Apr 15;156(1):159–165. doi: 10.1042/bj1560159

Hydrogen-ion titration studies on erythrocyte membranes.

C Hallam, J M Wrigglesworth
PMCID: PMC1163727  PMID: 8038

Abstract

1. H+ titration was used to detect the presence of ionizable groups on human erythrocyte plasma membranes. Between pH2.9 and 11.3, two significant peaks of H+ association/dissociation occur in the differential from of the titration curve, one at pH3. 1. And the other at pH10.3. 2. After disruption of membrane structure by exposure to high pH or by the addition of sodium dodecyl sulphate, maxima of H+ association/dissociation were seen at pH3.1,4.3,6.5,10.3 and 10.7. 3. Spectrophotometric assay and selective chemical treatments were used to identify several of the titratable residues. 4. The degree of eleectrostatic interaction between titratable charged groups was investigated by comparing the titration characteristics of the membranes before and after modification of membrane structure.

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Selected References

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  1. ABRAMSON M. B., KATZMAN R., WILSON C. E., GREGOR H. P. IONIC PROPERTIES OF AQUEOUS DISPERSIONS OF PHOSPHATIDIC ACID. J Biol Chem. 1964 Dec;239:4066–4072. [PubMed] [Google Scholar]
  2. BROWN A. D. HYDROGEN ION TITRATIONS OF INTACT AND DISSOLVED LIPOPROTEIN MEMBRANES. J Mol Biol. 1965 Jun;12:491–508. doi: 10.1016/s0022-2836(65)80272-8. [DOI] [PubMed] [Google Scholar]
  3. Bellin J. S., Yankus C. A. Influence of dye binding on the sensitized photooxidation of amino acids. Arch Biochem Biophys. 1968 Jan;123(1):18–28. doi: 10.1016/0003-9861(68)90099-4. [DOI] [PubMed] [Google Scholar]
  4. Butterworth P. H., Baum H., Porter J. W. A modification of the Ellman procedure for the estimation of protein sulfhydryl groups. Arch Biochem Biophys. 1967 Mar 20;118(3):716–723. doi: 10.1016/0003-9861(67)90409-2. [DOI] [PubMed] [Google Scholar]
  5. DODGE J. T., MITCHELL C., HANAHAN D. J. The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes. Arch Biochem Biophys. 1963 Jan;100:119–130. doi: 10.1016/0003-9861(63)90042-0. [DOI] [PubMed] [Google Scholar]
  6. Devenuto F., Ligon D. F., Friedrichsen D. H., Wilson H. L. Human erythrocyte membrane. Uptake of progesterone and chemical alterations. Biochim Biophys Acta. 1969 Oct 14;193(1):36–47. doi: 10.1016/0005-2736(69)90056-x. [DOI] [PubMed] [Google Scholar]
  7. EYLAR E. H., MADOFF M. A., BRODY O. V., ONCLEY J. L. The contribution of sialic acid to the surface charge of the erythrocyte. J Biol Chem. 1962 Jun;237:1992–2000. [PubMed] [Google Scholar]
  8. Fairbanks G., Steck T. L., Wallach D. F. Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry. 1971 Jun 22;10(13):2606–2617. doi: 10.1021/bi00789a030. [DOI] [PubMed] [Google Scholar]
  9. Forstner J., Manery J. F. Calcium binding by human erythrocyte membranes. Significance of carboxyl, amino and thiol groups. Biochem J. 1971 Nov;125(1):343–352. doi: 10.1042/bj1250343. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. GARVIN J. E., KARNOVSKY M. L. The titration of some phosphatides and related compounds in a non-aqueous medium. J Biol Chem. 1956 Jul;221(1):211–222. [PubMed] [Google Scholar]
  11. GORNALL A. G., BARDAWILL C. J., DAVID M. M. Determination of serum proteins by means of the biuret reaction. J Biol Chem. 1949 Feb;177(2):751–766. [PubMed] [Google Scholar]
  12. Gordesky S. E., Marinetti G. V., Love R. The reaction of chemical probes with the erythrocyte membrane. J Membr Biol. 1975;20(1-2):111–132. doi: 10.1007/BF01870631. [DOI] [PubMed] [Google Scholar]
  13. Habeeb A. J., Hiramoto R. Reaction of proteins with glutaraldehyde. Arch Biochem Biophys. 1968 Jul;126(1):16–26. doi: 10.1016/0003-9861(68)90554-7. [DOI] [PubMed] [Google Scholar]
  14. Haydon D. A., Seaman G. V. Electrokinetic studies on the ultrastructure of the human erythrocyte. I. Electrophoresis at high ionic strengths--the cell as a polyanion. Arch Biochem Biophys. 1967 Oct;122(1):126–136. doi: 10.1016/0003-9861(67)90131-2. [DOI] [PubMed] [Google Scholar]
  15. Holmgren J., Lindholm L., Lönnroth I. Interaction of cholera toxin and toxin derivatives with lymphocytes. I. Binding properties and interference with lectin-induced cellular stimulation. J Exp Med. 1974 Apr 1;139(4):801–819. doi: 10.1084/jem.139.4.801. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Kathan R. H., Adamany A. Comparison of human MM, NN, and MN blood group antigens. J Biol Chem. 1967 Apr 25;242(8):1716–1722. [PubMed] [Google Scholar]
  17. Knauf P. A., Rothstein A. Chemical modification of membranes. I. Effects of sulfhydryl and amino reactive reagents on anion and cation permeability of the human red blood cell. J Gen Physiol. 1971 Aug;58(2):190–210. doi: 10.1085/jgp.58.2.190. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Kryzhanovsky G. N. The mechanism of action of tetanus toxin: effect on synaptic processes and some particular features of toxin binding by the nervous tissue. Naunyn Schmiedebergs Arch Pharmacol. 1973;276(3-4):247–270. doi: 10.1007/BF00499880. [DOI] [PubMed] [Google Scholar]
  19. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  20. Nicolson G. L., Painter R. G. Anionic sites of human erythrocyte membranes. II. Antispectrin-induced transmembrane aggregation of the binding sites for positively charged colloidal particles. J Cell Biol. 1973 Nov;59(2 Pt 1):395–406. doi: 10.1083/jcb.59.2.395. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Passow H. Passive ion permeability of the erythrocyte membrane. Prog Biophys Mol Biol. 1969;19(2):423–467. [PubMed] [Google Scholar]
  22. Pinto da Silva P. Translational mobility of the membrane intercalated particles of human erythrocyte ghosts. pH-dependent, reversible aggregation. J Cell Biol. 1972 Jun;53(3):777–787. doi: 10.1083/jcb.53.3.777. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Rasmussen H. Cell communication, calcium ion, and cyclic adenosine monophosphate. Science. 1970 Oct 23;170(3956):404–412. doi: 10.1126/science.170.3956.404. [DOI] [PubMed] [Google Scholar]
  24. Roozemond R. C. The effect of fixation with formaldehyde and glutaraldehyde on the composition of phospholipids extractable from rat hypothalamus. J Histochem Cytochem. 1969 Jul;17(7):482–486. doi: 10.1177/17.7.482. [DOI] [PubMed] [Google Scholar]
  25. Rosenberg S. A., Guidotti G. The protein of human erythrocyte membranes. I. Preparation, solubilization, and partial characterization. J Biol Chem. 1968 Apr 25;243(8):1985–1992. [PubMed] [Google Scholar]
  26. SANUI H., CARVALHO A. P., PACE N. Relationship of hydrogen ion binding to sodium and potassium binding by rat liver cell microsomes and human erythrocyte ghosts. J Cell Comp Physiol. 1962 Jun;59:241–250. doi: 10.1002/jcp.1030590303. [DOI] [PubMed] [Google Scholar]
  27. Sharon N., Lis H. Lectins: cell-agglutinating and sugar-specific proteins. Science. 1972 Sep 15;177(4053):949–959. doi: 10.1126/science.177.4053.949. [DOI] [PubMed] [Google Scholar]
  28. Steck T. L. The organization of proteins in the human red blood cell membrane. A review. J Cell Biol. 1974 Jul;62(1):1–19. doi: 10.1083/jcb.62.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Tenforde T. Microelectrophoretic studies on the surface chemistry of erythrocytes. Adv Biol Med Phys. 1970;13:43–105. doi: 10.1016/b978-0-12-005213-4.50006-5. [DOI] [PubMed] [Google Scholar]
  30. WARREN L. The thiobarbituric acid assay of sialic acids. J Biol Chem. 1959 Aug;234(8):1971–1975. [PubMed] [Google Scholar]
  31. Wills M. C., Tinker D. O., Pinteric L. Proton binding by phosphatidyl inositol in aqueous dispersion. Biochim Biophys Acta. 1971 Aug 13;241(2):483–493. doi: 10.1016/0005-2736(71)90047-2. [DOI] [PubMed] [Google Scholar]
  32. Wrigglesworth J. M. Electrostatic interactions at the plasma membrane. Philos Trans R Soc Lond B Biol Sci. 1975 Jul 17;271(912):273–275. doi: 10.1098/rstb.1975.0051. [DOI] [PubMed] [Google Scholar]
  33. Zwaal R. F., Roelofsen B., Colley C. M. Localization of red cell membrane constituents. Biochim Biophys Acta. 1973 Sep 10;300(2):159–182. doi: 10.1016/0304-4157(73)90003-8. [DOI] [PubMed] [Google Scholar]

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