Abstract
Pyruvate transport into rat liver mitochondria is inhibited by a variety of thiol reagents. alpha-Cyanocinnamate and its derivates, potent and reversible inhibitors of pyruvate transport, react reversibly with mercaptoethanol and cysteine to form addition products. It is concluded that these inhibitors react with an essential thiol group on the pyruvate carrier.
Full text
PDFSelected References
These references are in PubMed. This may not be the complete list of references from this article.
- Halestrap A. P., Denton R. M. Specific inhibition of pyruvate transport in rat liver mitochondria and human erythrocytes by alpha-cyano-4-hydroxycinnamate. Biochem J. 1974 Feb;138(2):313–316. doi: 10.1042/bj1380313. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Halestrap A. P., Denton R. M. The specificity and metabolic implications of the inhibition of pyruvate transport in isolated mitochondria and intact tissue preparations by alpha-Cyano-4-hydroxycinnamate and related compounds. Biochem J. 1975 Apr;148(1):97–106. doi: 10.1042/bj1480097. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Halestrap A. P. The mitochondrial pyruvate carrier. Kinetics and specificity for substrates and inhibitors. Biochem J. 1975 Apr;148(1):85–96. doi: 10.1042/bj1480085. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Papa S., Francavilla A., Paradies G., Meduri B. The transport of pyruvate in rat liver mitochondria. FEBS Lett. 1971 Jan 30;12(5):285–288. doi: 10.1016/0014-5793(71)80200-4. [DOI] [PubMed] [Google Scholar]
- Papa S., Paradies G. On the mechanism of translocation of pyruvate and other monocarboxylic acids in rat-liver mitochondria. Eur J Biochem. 1974 Nov 1;49(1):265–274. doi: 10.1111/j.1432-1033.1974.tb03831.x. [DOI] [PubMed] [Google Scholar]
- Paradies G., Papa S. The transport of monocarboxylic oxoacids in rat liver mitochondria. FEBS Lett. 1975 Mar 15;52(1):149–152. doi: 10.1016/0014-5793(75)80659-4. [DOI] [PubMed] [Google Scholar]