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. 1976 Aug 1;157(2):503–506. doi: 10.1042/bj1570503

The formation of a stable complex between dissociated proteoglycan and hyaluronic acid in the absence of a link protein.

D A Swann, S Powell, J Broadhurst, E Sordillo, S Sotman
PMCID: PMC1163881  PMID: 134701

Abstract

A proteoglycan fraction prepared from bovine articular cartilage under dissociative conditions was shown to interact with three purified hyaluronic acid preparations to form stable complexes in the analytical ultracentrifuge. It is concluded from these experiments that, although link proteins are associated with hyaluronic acid and proteoglycans in complexes between these macromolecular constituents.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bienenstock H. Rheumatoid plantar synovial cysts. Ann Rheum Dis. 1975 Feb;34(1):98–99. doi: 10.1136/ard.34.1.98. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Furthmayr H., Timpl R. Characterization of collagen peptides by sodium dodecylsulfate-polyacrylamide electrophoresis. Anal Biochem. 1971 Jun;41(2):510–516. doi: 10.1016/0003-2697(71)90173-4. [DOI] [PubMed] [Google Scholar]
  3. Gregory J. D. Multiple aggregation factors in cartilage proteoglycan. Biochem J. 1973 Jun;133(2):383–386. doi: 10.1042/bj1330383. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hardingham T. E., Muir H. The specific interaction of hyaluronic acid with cartillage proteoglycans. Biochim Biophys Acta. 1972 Sep 15;279(2):401–405. doi: 10.1016/0304-4165(72)90160-2. [DOI] [PubMed] [Google Scholar]
  5. Hascall V. C., Heinegård D. Aggregation of cartilage proteoglycans. I. The role of hyaluronic acid. J Biol Chem. 1974 Jul 10;249(13):4232–4241. [PubMed] [Google Scholar]
  6. Hascall V. C., Heinegård D. Aggregation of cartilage proteoglycans. II. Oligosaccharide competitors of the proteoglycan-hyaluronic acid interaction. J Biol Chem. 1974 Jul 10;249(13):4242–4249. [PubMed] [Google Scholar]
  7. Hascall V. C., Sajdera S. W. Proteinpolysaccharide complex from bovine nasal cartilage. The function of glycoprotein in the formation of aggregates. J Biol Chem. 1969 May 10;244(9):2384–2396. [PubMed] [Google Scholar]
  8. Heinegård D., Hascall V. C. Aggregation of cartilage proteoglycans. 3. Characteristics of the proteins isolated from trypsin digests of aggregates. J Biol Chem. 1974 Jul 10;249(13):4250–4256. [PubMed] [Google Scholar]
  9. Sajdera S. W., Hascall V. C. Proteinpolysaccharide complex from bovine nasal cartilage. A comparison of low and high shear extraction procedures. J Biol Chem. 1969 Jan 10;244(1):77–87. [PubMed] [Google Scholar]
  10. Strider W., Pal S., Rosenberg L. Comparison of proteoglycans from bovine articular cartilage. Biochim Biophys Acta. 1975 Jan 30;379(1):271–281. doi: 10.1016/0005-2795(75)90030-6. [DOI] [PubMed] [Google Scholar]
  11. Swann D. A., Caulfield J. B. Studies on hyaluronic acid. V. Relationship between the protein content and viscosity of rooster comb dermis hyaluronic acid. Connect Tissue Res. 1975;4(1):31–39. doi: 10.3109/03008207509152195. [DOI] [PubMed] [Google Scholar]
  12. Swann D. A., Constable I. J. Vitreous structure. I. Distribution of hyaluronate and protein. Invest Ophthalmol. 1972 Mar;11(3):159–163. [PubMed] [Google Scholar]
  13. Swann D. A., Radin E. L. The molecular basis of articular lubrication. I. Purification and properties of a lubricating fraction from bovine synovial fluid. J Biol Chem. 1972 Dec 25;247(24):8069–8073. [PubMed] [Google Scholar]
  14. Swann D. A. Studies on hyaluronic acid. I. The preparation and properties of rooster comb hyaluronic acid. Biochim Biophys Acta. 1968 Feb 1;156(1):17–30. [PubMed] [Google Scholar]

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