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. 1976 Aug 1;157(2):517–520. doi: 10.1042/bj1570517

Isolation of (copper, zinc)-thioneins from the livers of copper-injected rats.

I Bremner, B W Young
PMCID: PMC1163885  PMID: 962883

Abstract

The mixed copper- and zinc-binding proteins, with mol.wt. of approx. 12000, induced in rat liver after injection of copper were isolated and characterized as metallothioneins. Three separate forms were obtained, with 7-11% total metal in the protein, equivalent to 2.6-1.6 cysteine residues/metal atom.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bloomer L. C., Sourkes T. L. The effect of copper loading on the distribution of copper in rat liver cytosol. Biochem Med. 1973 Aug;8(1):78–91. doi: 10.1016/0006-2944(73)90011-2. [DOI] [PubMed] [Google Scholar]
  2. Bremner I., Davies N. T. Studies on the appearance of a hepatic copper-binding protein in normal and zinc-deficient rats. Br J Nutr. 1976 Jul;36(1):101–112. doi: 10.1079/bjn19760061. [DOI] [PubMed] [Google Scholar]
  3. Bremner I., Davies N. T. The induction of metallothionein in rat liver by zinc injection and restriction of food intake. Biochem J. 1975 Sep;149(3):733–738. doi: 10.1042/bj1490733. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bremner I. Heavy metal toxicities. Q Rev Biophys. 1974 Feb;7(1):75–124. doi: 10.1017/s0033583500001359. [DOI] [PubMed] [Google Scholar]
  5. Bremner I., Young B. W. Isolation of (copper, zinc-) thioneins from pig liver. Biochem J. 1976 Jun 1;155(3):631–635. doi: 10.1042/bj1550631. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Bühler R. H., Kägi J. H. Human hepatic metallothioneins. FEBS Lett. 1974 Feb 15;39(2):229–234. doi: 10.1016/0014-5793(74)80057-8. [DOI] [PubMed] [Google Scholar]
  7. JOCELYN P. C. The effect of glutathione on protein sulphydryl groups in rat-liver homogenates. Biochem J. 1962 Dec;85:480–485. doi: 10.1042/bj0850480. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Nordberg G. F., Nordberg M., Piscator M., Vesterberg O. Separation of two forms of rabbit metallothionein by isoelectric focusing. Biochem J. 1972 Feb;126(3):491–498. doi: 10.1042/bj1260491. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Prinz R., Weser U. Cuprodoxin. FEBS Lett. 1975 Jun 15;54(2):224–229. doi: 10.1016/0014-5793(75)80079-2. [DOI] [PubMed] [Google Scholar]
  10. Riordan J. R., Gower I. Purification of low molecular weight copper proteins from copper loaded liver. Biochem Biophys Res Commun. 1975 Sep 16;66(2):678–686. doi: 10.1016/0006-291x(75)90563-x. [DOI] [PubMed] [Google Scholar]
  11. Winge D. R., Premakumar R., Rajagopalan K. V. Metal-induced formation of metallothionein in rat liver. Arch Biochem Biophys. 1975 Sep;170(1):242–252. doi: 10.1016/0003-9861(75)90115-0. [DOI] [PubMed] [Google Scholar]
  12. Winge D. R., Premakumar R., Wiley R. D., Rajagopalan K. V. Copper-chelatin: purification and properties of a copper-binding protein from rat liver. Arch Biochem Biophys. 1975 Sep;170(1):253–266. doi: 10.1016/0003-9861(75)90116-2. [DOI] [PubMed] [Google Scholar]

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