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. 1976 Sep 1;157(3):785–787. doi: 10.1042/bj1570785

Activation by cholera toxin of adenylate cyclase solubilized from rat liver.

S Heyningen
PMCID: PMC1163924  PMID: 985419

Abstract

Cholera toxin, or peptide A1 from the toxin, activates adenylate cyclase solubilized from rat liver with Lubrol PX, provided that cell sap, NAD+, ATP and thiol-group-containing compounds are present. The activation is abolished by antisera to whole toxin, but not to subunit B.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beckman B., Flores J., Witkum P. A., Sharp G. W. Studies on the mode of action of cholera toxin. Effects on solubilized adenylate cyclase. J Clin Invest. 1974 Apr;53(4):1202–1205. doi: 10.1172/JCI107660. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bitensky M. W., Wheeler M. A., Mehta H., Miki N. Cholera toxin activation of adenylate cyclase in cancer cell membrane fragments. Proc Natl Acad Sci U S A. 1975 Jul;72(7):2572–2576. doi: 10.1073/pnas.72.7.2572. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brown B. L., Albano J. D., Ekins R. P., Sgherzi A. M. A simple and sensitive saturation assay method for the measurement of adenosine 3':5'-cyclic monophosphate. Biochem J. 1971 Feb;121(3):561–562. doi: 10.1042/bj1210561. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cuatrecasas P. Gangliosides and membrane receptors for cholera toxin. Biochemistry. 1973 Aug 28;12(18):3558–3566. doi: 10.1021/bi00742a032. [DOI] [PubMed] [Google Scholar]
  5. Finkelstein R. A., Boesman M., Neoh S. H., LaRue M. K., Delaney R. Dissociation and recombination of the subunits of the cholera enterotoxin (choleragen). J Immunol. 1974 Jul;113(1):145–150. [PubMed] [Google Scholar]
  6. Flores J., Witkum P., Sharp G. W. Activation of adenylate cyclase by cholera toxin in rat liver homogenates. J Clin Invest. 1976 Feb;57(2):450–458. doi: 10.1172/JCI108296. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gill D. M. Involvement of nicotinamide adenine dinucleotide in the action of cholera toxin in vitro. Proc Natl Acad Sci U S A. 1975 Jun;72(6):2064–2068. doi: 10.1073/pnas.72.6.2064. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gill D. M., King C. A. The mechanism of action of cholera toxin in pigeon erythrocyte lysates. J Biol Chem. 1975 Aug 25;250(16):6424–6432. [PubMed] [Google Scholar]
  9. Gill D. M. Multiple roles of erythrocyte supernatant in the activation of adenylate cyclase by Vibrio cholerae toxin in vitro. J Infect Dis. 1976 Mar;133 (Suppl):55–63. doi: 10.1093/infdis/133.supplement_1.s55. [DOI] [PubMed] [Google Scholar]
  10. Gill D. M. The arrangement of subunits in cholera toxin. Biochemistry. 1976 Mar 23;15(6):1242–1248. doi: 10.1021/bi00651a011. [DOI] [PubMed] [Google Scholar]
  11. Heyningen S Van Cholera toxin: interaction of subunits with ganglioside GM1. Science. 1974 Feb 15;183(4125):656–657. doi: 10.1126/science.183.4125.656. [DOI] [PubMed] [Google Scholar]
  12. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  13. Ledley F. D., Mullin B. R., Lee G., Aloj S. M., Fishman P. H., Hunt L. T., Dayhoff M. O., Kohn L. D. Sequence similarity between cholera toxin and glycoprotein hormones: implications for structure activity relationship and mechanism of action. Biochem Biophys Res Commun. 1976 Apr 19;69(4):852–859. doi: 10.1016/0006-291x(76)90452-6. [DOI] [PubMed] [Google Scholar]
  14. Lönnroth I., Holmgren J. Protein reagent modification of cholera toxin: characterization of effects on antigenic, receptor-binding and toxic properties. J Gen Microbiol. 1975 Dec;91(2):263–277. doi: 10.1099/00221287-91-2-263. [DOI] [PubMed] [Google Scholar]
  15. Mullin B. R., Fishman P. H., Lee G., Aloj S. M., Ledley F. D., Winand R. J., Kohn L. D., Brady R. O. Thyrotropin-ganglioside interactions and their relationship to the structure and function of thyrotropin receptors. Proc Natl Acad Sci U S A. 1976 Mar;73(3):842–846. doi: 10.1073/pnas.73.3.842. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Révész T., Greaves M. Ligand-induced redistribution of lymphocyte membrane ganglioside GM1. Nature. 1975 Sep 11;257(5522):103–106. doi: 10.1038/257103a0. [DOI] [PubMed] [Google Scholar]
  17. Sahyoun N., Cuatrecasas P. Mechanism of activation of adenylate cyclase by cholera toxin. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3438–3442. doi: 10.1073/pnas.72.9.3438. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sattler J., Wiegandt H. Studies of the subunit structure of choleragen. Eur J Biochem. 1975 Sep 1;57(1):309–316. doi: 10.1111/j.1432-1033.1975.tb02302.x. [DOI] [PubMed] [Google Scholar]
  19. Van Heyningen S., King C. A. Short communications. Subunit A from cholera toxin is an activator of adenylate cyclase in pigeon erythrocytes. Biochem J. 1975 Jan;146(1):269–271. doi: 10.1042/bj1460269. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Van Heyningen W. E., Carpenter C. C., Pierce N. F., Greenough W. B., 3rd Deactivation of cholera toxin by ganglioside. J Infect Dis. 1971 Oct;124(4):415–418. doi: 10.1093/infdis/124.4.415. [DOI] [PubMed] [Google Scholar]

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