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. 1976 Aug 15;158(2):451–456. doi: 10.1042/bj1580451

The composition of an unusual precursor of 50 S ribosomes in a mutant of Escherichia coli.

F Markey, P F Sims, D G Wild
PMCID: PMC1163988  PMID: 791272

Abstract

Escherichia coli strain 15--28 is a mutant which during exponential growth contains large amounts of a '47S' ribonucleoprotein precursor to 50S ribosomes. The '47S particles' are more sensitive to ribonuclease than are 50S ribosomes. The 23 S RNA of 47S particles may be slightly undermethylated, but cannot be distinguished from the 23S RNA of 50S ribosomes by sedimentation or electrophoresis. Isolated particles have 10--15% less protein than do 50S ribosomes; proteins L16, L28 and L33 are absent. Comparison with precursor particles studied by other workers in wild-type strains of E. coli suggests that the assembly of 50S ribosomes in strain 15--28 is atypical.

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Selected References

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