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. 1976 Sep 15;158(3):529–533. doi: 10.1042/bj1580529

Altered polyamine metabolism in the PRO/Re strain of inbred mice.

C A Manen, R L Blake, D H Russell
PMCID: PMC1164006  PMID: 985447

Abstract

The PRO/Re strain of inbred mice are characterized by abnormally high concentrations of proline in both blood (hyperprolinaemia) and urine (prolinuria). They excrete increased amounts of polyamines in their urine. Male PRO/Re mice excreted putrescine at 175% and spermidine at 300% the amount of male C57BL/6J controls. Female PRO/Re mice excreted putrescine at 115% and spermidine at 150% of the amount in the urine of female controls. Examination of the enzymes involved in polyamine biosynthesis revealed that ornithine decarboxylase, the initial enzyme in the polyamine-biosynthetic pathway, was increased by 150% in the kidneys and by 100% in the liver of male PRO/Re mice. There was no significant difference between PRO/Re and C57BL/6J male mice for either putrescine- or spermidine-stimulated S-adenosylmethionine decarboxylase activity. Female PRO/Re mice showed no significant difference from female C57BL/6J mice for any of the enzymes examined. When the concentrations of the polyamines in the tissues of the PRO/Re mice were determined, spermidine and spermine concentrations in the kidneys of the male PRO/Re mice were twice those of the controls. Spermidine concentration in the livers of both male and female PRO/Re mice was approx. 130% that of the controls. Polyamine concentrations in the brains were similar in controls and mutants. The increased polyamine biosynthesis and excretion in the PRO/Re mutant mice may be a mechanism to decrease the extent of proline accumulation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blake R. L. Animal model for hyperprolinaemia: deficiency of mouse proline oxidase activity. Biochem J. 1972 Oct;129(4):987–989. doi: 10.1042/bj1290987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Blake R. L., Russell E. S. Hyperprolinemia and prolinuria in a new inbred strain of mice, PRO-Re. Science. 1972 May 19;176(4036):809–811. doi: 10.1126/science.176.4036.809. [DOI] [PubMed] [Google Scholar]
  3. Clark R. B., Fair W. R. The selective in vivo incorporation and metabolism of radioactive putrescine in the adult male rat. J Nucl Med. 1975 May;16(5):337–342. [PubMed] [Google Scholar]
  4. EFRON M. L. FAMILIAL HYPERPROLINEMIA. REPORT OF A SECOND CASE, ASSOCIATED WITH CONGENITAL RENAL MALFORMATIONS, HEREDITARY HEMATURIA AND MILD MENTAL RETARDATION, WITH DEMONSTRATION OF AN ENZYME DEFECT. N Engl J Med. 1965 Jun 17;272:1243–1254. doi: 10.1056/NEJM196506172722401. [DOI] [PubMed] [Google Scholar]
  5. Jänne J. Studies on the biosynthetic pathway of polyamines in rat liver. Acta Physiol Scand Suppl. 1967;300:1–71. [PubMed] [Google Scholar]
  6. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  7. Liappis N. Geschlechtsspezifische Unterschiede der freien Aminosäuren im Urin von Erwachsenen. Z Klin Chem Klin Biochem. 1973 Jul;11(7):279–285. [PubMed] [Google Scholar]
  8. Marton L. J., Russell D. H., Levy C. C. Measurement of putrescine, spermidine, and spermine in physiological fluids by use of an amino acid analyzer. Clin Chem. 1973 Aug;19(8):923–926. [PubMed] [Google Scholar]
  9. ROSENTHAL S. M., TABOR C. W. The pharmacology of spermine and spermidine; distribution and excretion. J Pharmacol Exp Ther. 1956 Feb;116(2):131–138. [PubMed] [Google Scholar]
  10. Russell D. H., Durie B. G., Salmon S. E. Polyamines as predictors of success and failure in cancer chemotherapy. Lancet. 1975 Oct 25;2(7939):797–799. doi: 10.1016/s0140-6736(75)80009-2. [DOI] [PubMed] [Google Scholar]
  11. Russell D. H. Effects of methotrexate and cytosine arabinoside on polyamine metabolism in a mouse L1210 leukemia. Cancer Res. 1972 Nov;32(11):2459–2462. [PubMed] [Google Scholar]
  12. Russell D. H., Gullino P. M., Marton L. J., LeGendre S. M. Polyamine depletion of the MTW9 mammary tumor and subsequent elevation of spermidine in the sera of tumor-bearing rats as a biochemical marker of tumor regression. Cancer Res. 1974 Sep;34(9):2378–2381. [PubMed] [Google Scholar]
  13. Russell D. H., Medina V. J., Snyder S. H. The dynamics of synthesis and degradation of polyamines in normal and regenerating rat liver and brain. J Biol Chem. 1970 Dec 25;245(24):6732–6738. [PubMed] [Google Scholar]
  14. Russell D. H., Russell S. D. Relative usefulness of measuring polyamines in serum, plasma, and urine as biochemical markers of cancer. Clin Chem. 1975 Jun;21(7):860–863. [PubMed] [Google Scholar]
  15. Russell D. H., Snyder S. H. Amine synthesis in regenerating rat liver: effect of hypophysectomy and growth hormone on ornithine decarboxylase. Endocrinology. 1969 Feb;84(2):223–228. doi: 10.1210/endo-84-2-223. [DOI] [PubMed] [Google Scholar]
  16. Siimes M. Studies on the metabolism of 1,4-14C-spermidine and 1,4-14C-spermine in the rat. Acta Physiol Scand Suppl. 1967;298:1–66. [PubMed] [Google Scholar]
  17. TABOR C. W., ROSENTHAL S. M. Pharmacology of spermine and spermidine; some effects on animals and bacteria. J Pharmacol Exp Ther. 1956 Feb;116(2):139–155. [PubMed] [Google Scholar]

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