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. 1976 Oct 1;159(1):65–70. doi: 10.1042/bj1590065

Studies on ligand binding to bovine liver uridine diphosphate glucose pyrophosphorylase.

R A Stevens, C F Phelps
PMCID: PMC1164038  PMID: 999644

Abstract

A procedure for the preparation of crystalline UDP-glucose pyrophosphorylase is described. K(s) values for UDP-glucose and UTP were determined as 7 and 20 muM respectively, the latter being confirmed by three methods. By assuming an octameric structure, 1 mol of enzyme subunit bound 1 mol of substrate. The metal-ion activator, Mg2+, did not affect the equilibrium between nucleotide and enzyme. A substrate analogue, alphabeta-methylene-UTP, was synthesized and had the same K(s) value as UTP. In its presence, the K(s) for glucose 1-phosphate decreased by two orders of magnitude, thus confirming a compulsory binding order and excluding an uridylated enzyme intermediate. The results are discussed with respect to their implications in vivo.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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