Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1976 Oct 15;160(1):11–21. doi: 10.1042/bj1600011

The relationship between adrogen receptors and the hormonally controlled responses of rat ventral prostate.

E Van Doorn, S Craven, N Bruchovsky
PMCID: PMC1164195  PMID: 1008839

Abstract

1. The administration of dihydrotestosterone to rats orchidectomized 7 days previously stimulated the synthesis of nuclear receptor in prostatic cells several hours in advance of DNA synthesis and mitosis. 2. The synthesis of nuclear receptor is tightly coupled to cell proliferation; consequently, in resting cells, there is no further net synthesis of nuclear receptor above the maximum of approx. 8000 molecules/cell. 3. After orchidectomy a rapid decline in the concentration of free androgen in the nuceus and a slower decline in the concentration of nuclear receptor are observed. 4. Owing to the apparent scarcity of receptor-inactivating factors in the nucleus, and the inverse relationship between amounts of nuclear and cytoplasmic receptors, it is concluded that the nuclear receptor is discharged into the cytoplasm after orchidectomy. 5. The formation of the cytoplasmic receptor is an early event preceding the onset of cellular autolysis. 6. Regressing prostate develops the capacity to eliminate cytoplasmic receptor, and this capacity is retained by the regenerating prostate for at least 14 days. 7. The synthesis of nuclear receptor in early G1 phase may control the entry of cells into the cell cycle and the prolonged retention of receptor in the nucleus may prevent the activation of autophagic processes.

Full text

PDF
11

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bruchovsky N. Comparison of the metabolites formed in rat prostate following the in vivo administration of seven natural androgens. Endocrinology. 1971 Nov;89(5):1212–1222. doi: 10.1210/endo-89-5-1212. [DOI] [PubMed] [Google Scholar]
  2. Bruchovsky N., Craven S. Prostatic involution: effect on androgen receptors and intracellular androgen transport. Biochem Biophys Res Commun. 1975 Feb 17;62(4):837–843. doi: 10.1016/0006-291x(75)90398-8. [DOI] [PubMed] [Google Scholar]
  3. Bruchovsky N., Lesser B. Control of proliferative growth in androgen responsive organs and neoplasms. Adv Sex Horm Res. 1976;2:1–55. [PubMed] [Google Scholar]
  4. Bruchovsky N., Lesser B., Van Doorn E., Craven S. Hormonal effects on cell proliferation in rat prostate. Vitam Horm. 1975;33:61–102. doi: 10.1016/s0083-6729(08)60951-6. [DOI] [PubMed] [Google Scholar]
  5. Bruchovsky N., Rennie P. S., Vanson A. Studies on the regulation of the concentration of androgens and androgen receptors in nuclei of prostatic cells. Biochim Biophys Acta. 1975 Jun 25;394(2):248–266. doi: 10.1016/0005-2736(75)90263-1. [DOI] [PubMed] [Google Scholar]
  6. Dahl E., Kjaerheim A. The ultrastructure of the accessory sex organs of the male rat. II. The post-castration involution of the ventral, lateral and the dorsal prostate. Z Zellforsch Mikrosk Anat. 1973 Nov 5;144(2):167–178. doi: 10.1007/BF00307300. [DOI] [PubMed] [Google Scholar]
  7. Davies P., Griffiths K. Further studies on the stimulation of prostatic ribonucleic acid polymerase by x-5 alpha-dihydrotestosterone-receptor complexes. J Endocrinol. 1974 Aug;62(2):385–400. doi: 10.1677/joe.0.0620385. [DOI] [PubMed] [Google Scholar]
  8. Davies P., Griffiths K. Similarities between 5alpha-dihydrotestosterone-receptor complexes from human and rat prostatic tissue: effects on RNA polymerase activity. Mol Cell Endocrinol. 1975 Aug;3(2):143–164. doi: 10.1016/0303-7207(75)90060-x. [DOI] [PubMed] [Google Scholar]
  9. Davies P., Griffiths K. Stimulation of ribonucleic acid polymerase activity in vitro by prostatic steroid-protein receptor complexes. Biochem J. 1973 Nov;136(3):611–622. doi: 10.1042/bj1360611. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Helminen H. J., Ericsson J. L., Arborgh B. Differing patterns of acid phosphatase and cathepsin D activities in the rat ventral prostate gland during castration-induced prostatic involution. Acta Endocrinol (Copenh) 1972 Apr;69(4):747–761. doi: 10.1530/acta.0.0690747. [DOI] [PubMed] [Google Scholar]
  11. Hu A. L., Loor R. M., Wang T. Y. Purification of a 3S cytosol androgen receptor from rat prostate that stimulates DNA-dependent RNA synthesis in vitro. Biochem Biophys Res Commun. 1975 Aug 18;65(4):1327–1333. doi: 10.1016/s0006-291x(75)80375-5. [DOI] [PubMed] [Google Scholar]
  12. Ichii S., Izawa M., Murakami N. Hormonal regulation of protein synthesis in rat ventral prostate. Endocrinol Jpn. 1974 Jun;21(3):267–274. doi: 10.1507/endocrj1954.21.267. [DOI] [PubMed] [Google Scholar]
  13. Katsumata M., Goldman A. S. Separation of multiple dihydrotestosterone receptors in rat ventral prostate by a novel micromethod of electrofocusing. Blocking action of cyproterone acetate and uptake by nuclear chromatin. Biochim Biophys Acta. 1974 Jul 7;359(1):112–129. doi: 10.1016/0005-2795(74)90137-8. [DOI] [PubMed] [Google Scholar]
  14. Lesser B., Bruchovsky N. Effect of duration of the period after castration on the response of the rat ventral prostate to androgens. Biochem J. 1974 Aug;142(2):429–431. doi: 10.1042/bj1420429. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lesser B., Bruchovsky N. The effects of 5alpha-dihydrotestosterone on the kinetics of cell proliferation in rat prostate. Biochem J. 1974 Sep;142(3):483–489. doi: 10.1042/bj1420483. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lesser B., Bruchovsky N. The effects of testosterone, 5 -dihydrotestosterone and adenosine 3',5'-monophosphate on cell proliferation and differentiation in rat prostate. Biochim Biophys Acta. 1973 May 18;308(3):426–437. doi: 10.1016/0005-2787(73)90336-5. [DOI] [PubMed] [Google Scholar]
  17. Liang T., Liao S. A very rapid effect of androgen on initiation of protein synthesis in prostate. Proc Natl Acad Sci U S A. 1975 Feb;72(2):706–709. doi: 10.1073/pnas.72.2.706. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Liao S. Cellular receptors and mechanisms of action of steroid hormones. Int Rev Cytol. 1975;41:87–172. doi: 10.1016/s0074-7696(08)60967-5. [DOI] [PubMed] [Google Scholar]
  19. MARTIN R. G., AMES B. N. A method for determining the sedimentation behavior of enzymes: application to protein mixtures. J Biol Chem. 1961 May;236:1372–1379. [PubMed] [Google Scholar]
  20. Mainwaring W. I., Jones D. M. Influence of receptor complexes on the properties of prostate chromatin, including its transcription by RNA polymerase. J Steroid Biochem. 1975 Mar-Apr;6(3-4):475–481. doi: 10.1016/0022-4731(75)90175-2. [DOI] [PubMed] [Google Scholar]
  21. Paris J. E., Brandes D., Anton E. B., Barnard S. Effect of castration on histochemistry and biochemistry of acid hydrolases in rat prostate gland. J Natl Cancer Inst. 1972 Dec;49(6):1685–1696. doi: 10.1093/jnci/49.6.1685. [DOI] [PubMed] [Google Scholar]
  22. Rennie P. S., Symes E. K., Mainwaring W. J. The androgenic regulation of the activities of enzymes engaged in the synthesis of deoxyribonucleic acid in rat ventral prostate gland. Biochem J. 1975 Oct;152(1):1–16. doi: 10.1042/bj1520001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Rennie P., Bruchovsky N. In vitro and in vivo studies on the functional significance of androgen receptors in rat prostate. J Biol Chem. 1972 Mar 10;247(5):1546–1554. [PubMed] [Google Scholar]
  24. Rennie P., Bruchovsky N. Studies on the relationship between androgen receptors and the transport of androgens in rat prostate. J Biol Chem. 1973 May 10;248(9):3288–3297. [PubMed] [Google Scholar]
  25. Szalay R., Krieg M., Schmidt H., Voigt K. D. Metabolism and mode of action of androgens in target tissues of male rats. V. Uptake and metabolism of cyproterone acetate and its influence on the uptake and metabolism of testosterone and 5alpha-dihydrotestosterone in target organs and peripheral tissues. Acta Endocrinol (Copenh) 1975 Nov;80(3):592–602. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES