Skip to main content
Biochemical Journal logoLink to Biochemical Journal
. 1976 Oct 15;160(1):113–115. doi: 10.1042/bj1600113

Isolation of pure cholinergic nerve endings from the electric organ of Torpedo marmorata.

M Israël, R Manaranche, P Mastour-Frachon, N Morel
PMCID: PMC1164206  PMID: 1008840

Abstract

A rapid method for the preparation of highly purified cholinergic nerve endings from the electric organ of Torpedo is described. The endings retain their cytoplasmic components, as shown by biochemical and morphological observations. The homogeneity of these synaptosomes make them a useful tool for further studies.

Full text

PDF

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ELLMAN G. L., COURTNEY K. D., ANDRES V., Jr, FEATHER-STONE R. M. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol. 1961 Jul;7:88–95. doi: 10.1016/0006-2952(61)90145-9. [DOI] [PubMed] [Google Scholar]
  2. Fonnum F. A rapid radiochemical method for the determination of choline acetyltransferase. J Neurochem. 1975 Feb;24(2):407–409. doi: 10.1111/j.1471-4159.1975.tb11895.x. [DOI] [PubMed] [Google Scholar]
  3. Israel M., Gautron J., Lesbats B. Isolement des vésicules synaptiques de l'organe électrique de la Torpille et localisation de l'acétylcholine à leur niveau. C R Acad Sci Hebd Seances Acad Sci D. 1968 Jan 15;266(3):273–275. [PubMed] [Google Scholar]
  4. Israël M., Gautron J., Lesbats B. Fractionnement de l'organe electrique de la torpille: localisation subcellulaire de l'acetylcholine. J Neurochem. 1970 Oct;17(10):1441–1450. doi: 10.1111/j.1471-4159.1970.tb00511.x. [DOI] [PubMed] [Google Scholar]
  5. Israël M. Localisation de l'acétylcholine des synapses myoneurales et nerf-électroplaque. Arch Anat Microsc Morphol Exp. 1970 Apr-Jun;59(2):67–98. [PubMed] [Google Scholar]
  6. JOHNSON M. K., WHITTAKER V. P. LACTATE DEHYDROGENASE AS A CYTOPLASMIC MARKER IN BRAIN. Biochem J. 1963 Sep;88:404–409. doi: 10.1042/bj0880404. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. WHITTAKER V. P. The isolation and characterization of acetylcholine-containing particles from brain. Biochem J. 1959 Aug;72:694–706. doi: 10.1042/bj0720694. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Whittaker V. P., Essman W. B., Dowe G. H. The isolation of pure cholinergic synaptic vesicles from the electric organs of elasmobranch fish of the family Torpedinidae. Biochem J. 1972 Jul;128(4):833–845. doi: 10.1042/bj1280833. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES