Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1976 Nov 15;160(2):413–416. doi: 10.1042/bj1600413

Acute effects in vivo of anti-insulin serum on rates of fatty acid synthesis and activities of acetyl-coenzyme A carboxylase and pyruvate dehydrogenase in liver and epididymal adipose tissue of fed rats.

D Stansbie, R W Brownsey, M Crettaz, R M Denton
PMCID: PMC1164250  PMID: 12755

Abstract

Plasma insulin concentrations in fed rats were altered acutely by administration of glucose or anti-insulin serum. Rates of fatty acid synthesis in adipose tissue and liver were estimated from the incorporation of 3H from 3H2O. In the adipose tissue dehydrogenase and acetyl-CoA carboxylase were evident. In liver, although changes in rates of fatty acid synthesis were found, the initial activity of pyruvate dehydrogenase did not alter, but small parallel changes in acetyl-CoA carboxylase activity were observed.

Full text

PDF
413

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CROFFORD O. B., RENOLD A. E. GLUCOSE UPTAKE BY INCUBATED RAT EPIDIDYMAL ADIPOSE TISSUE. RATE-LIMITING STEPS AND SITE OF INSULIN ACTION. J Biol Chem. 1965 Jan;240:14–21. [PubMed] [Google Scholar]
  2. Coore H. G., Denton R. M., Martin B. R., Randle P. J. Regulation of adipose tissue pyruvate dehydrogenase by insulin and other hormones. Biochem J. 1971 Nov;125(1):115–127. doi: 10.1042/bj1250115. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Denton R. M. Hormonal regulation of fatty acid synthesis in adipose tissue through changes in the activities of pyruvate dehydrogenase (EC 1.2.4.1) and acetyl-CoA carboxylase (EC 6.4.1.2). Proc Nutr Soc. 1975 Dec;34(3):217–224. doi: 10.1079/pns19750042. [DOI] [PubMed] [Google Scholar]
  4. Denton R. M., Randle P. J., Bridges B. J., Cooper R. H., Kerbey A. L., Pask H. T., Severson D. L., Stansbie D., Whitehouse S. Regulation of mammalian pyruvate dehydrogenase. Mol Cell Biochem. 1975 Oct 31;9(1):27–53. doi: 10.1007/BF01731731. [DOI] [PubMed] [Google Scholar]
  5. Haft D. E. Effects of insulin on metabolism of perfused livers of rats made acutely diabetic by anti-insulin serum injection. Diabetes. 1968 May;17(5):251–255. doi: 10.2337/diab.17.5.251. [DOI] [PubMed] [Google Scholar]
  6. Halestrap A. P., Denton R. M. Hormonal regulation of adipose-tissue acetyl-Coenzyme A carboxylase by changes in the polymeric state of the enzyme. The role of long-chain fatty acyl-Coenzyme A thioesters and citrate. Biochem J. 1974 Aug;142(2):365–377. doi: 10.1042/bj1420365. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Halestrap A. P., Denton R. M. Insulin and the regulation of adipose tissue acetyl-coenzyme A carboxylase. Biochem J. 1973 Mar;132(3):509–517. doi: 10.1042/bj1320509. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hems D. A., Rath E. A., Verrinder T. R. Fatty acid synthesis in liver and adipose tissue of normal and genetically obese (ob/ob) mice during the 24-hour cycle. Biochem J. 1975 Aug;150(2):167–173. doi: 10.1042/bj1500167. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Jungas R. L. Fatty acid synthesis in adipose tissue incubated in tritiated water. Biochemistry. 1968 Oct;7(10):3708–3717. doi: 10.1021/bi00850a050. [DOI] [PubMed] [Google Scholar]
  10. Jungas R. L. Hormonal regulation of pyruvate dehydrogenase. Metabolism. 1971 Jan;20(1):43–53. doi: 10.1016/0026-0495(71)90058-8. [DOI] [PubMed] [Google Scholar]
  11. Linn T. C., Pettit F. H., Hucho F., Reed L. J. Alpha-keto acid dehydrogenase complexes. XI. Comparative studies of regulatory properties of the pyruvate dehydrogenase complexes from kidney, heart, and liver mitochondria. Proc Natl Acad Sci U S A. 1969 Sep;64(1):227–234. doi: 10.1073/pnas.64.1.227. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Linn T. C., Pettit F. H., Reed L. J. Alpha-keto acid dehydrogenase complexes. X. Regulation of the activity of the pyruvate dehydrogenase complex from beef kidney mitochondria by phosphorylation and dephosphorylation. Proc Natl Acad Sci U S A. 1969 Jan;62(1):234–241. doi: 10.1073/pnas.62.1.234. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lowenstein J. M. Effect of (-)-hydroxycitrate on fatty acid synthesis by rat liver in vivo. J Biol Chem. 1971 Feb 10;246(3):629–632. [PubMed] [Google Scholar]
  14. Patzelt C., Löffler G., Wieland O. H. Interconversion of pyruvate dehydrogenase in the isolated perfused rat liver. Eur J Biochem. 1973 Feb 15;33(1):117–122. doi: 10.1111/j.1432-1033.1973.tb02662.x. [DOI] [PubMed] [Google Scholar]
  15. ROBINSON B. H., WRIGHT P. H. Guinea-pig anti-insulin serum. J Physiol. 1961 Feb;155:302–310. doi: 10.1113/jphysiol.1961.sp006628. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Stansbie D., Denton R. M., Bridges B. J., Pask H. T., Randle P. J. Regulation of pyruvate dehydrogenase and pyruvate dehydrogenase phosphate phosphatase activity in rat epididymal fat-pads. Effects of starvation, alloxan-diabetes and high-fat diet. Biochem J. 1976 Jan 15;154(1):225–236. doi: 10.1042/bj1540225. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Taylor S. I., Mukherjee C., Jungas R. L. Studies on the mechanism of activation of adipose tissue pyruvate dehydrogenase by insulin. J Biol Chem. 1973 Jan 10;248(1):73–81. [PubMed] [Google Scholar]
  18. Vinten J., Gliemann J., Osterlind K. Exchange of 3-O-methylglucose in isolated fat cells. Concentration dependence and effect of insulin. J Biol Chem. 1976 Feb 10;251(3):794–800. [PubMed] [Google Scholar]
  19. Volpe J. J., Vagelos P. R. Saturated fatty acid biosynthesis and its regulation. Annu Rev Biochem. 1973;42:21–60. doi: 10.1146/annurev.bi.42.070173.000321. [DOI] [PubMed] [Google Scholar]
  20. Weiss L., Löffler G., Schirmann A., Wieland O. Control of pyruvate dehydrogenase interconversion in adipose tissue by insulin. FEBS Lett. 1971 Jun 24;15(3):229–231. doi: 10.1016/0014-5793(71)80318-6. [DOI] [PubMed] [Google Scholar]
  21. Wieland O. H., Patzelt C., Löffler G. Active and inactive forms of pyruvate dehydrogenase in rat liver. Effect of starvation and refeeding and of insulin treatment on pyruvate-dehydrogenase interconversion. Eur J Biochem. 1972 Apr 11;26(3):426–433. doi: 10.1111/j.1432-1033.1972.tb01783.x. [DOI] [PubMed] [Google Scholar]
  22. Windmueller H. G., Spaeth A. E. De novo synthesis of fatty acid in perfused rat liver as a determinant of plasma lipoprotein production. Arch Biochem Biophys. 1967 Nov;122(2):362–369. doi: 10.1016/0003-9861(67)90206-8. [DOI] [PubMed] [Google Scholar]
  23. Windmueller H. G., Spaeth A. E. Perfusion in situ with tritium oxide to measure hepatic lipogenesis and lipid secretion. Normal and orotic acid-fed rats. J Biol Chem. 1966 Jun 25;241(12):2891–2899. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES