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. 1976 Dec 15;160(3):463–466. doi: 10.1042/bj1600463

Total plasmalogens and O-(acylalkylglycerophosphoryl) ethanolamine from labelled hexadecanol and palmitate during hypoxia and anoxia in rat heart.

J L Rabinowitz, E S Hercker
PMCID: PMC1164261  PMID: 1016235

Abstract

By the use of the Langendorff technique, surviving isolated rat hearts were perfused with [1-14 C] palmitate, [1-14C] hexadecanol or [1-14C,1-3H] hexadecanol under normal or anoxic conditions. After perfusion for 30min with either precursor, when oxygenated or in an hypoxic condition, or when 1mM-KCN was included in the system, the heart tissues showed no significant chemical changes in their content of total lipids, total phospholipids or total ethanolamine-containing phospholipids. Changes were observed in the ratio of alkyl-to alk-1-enyl-glycerophosphorylethanolamine in the tissue perfused with N2+CO1 plus CN-. A slight increase from 4.0+/-0.3 to 4.9+/-0.2% in alkyl derivatives and a decrease from 11.2+/-0.4 to 9.4+/-0.3% in alk-1-enyl derivatives was observed. The incorporation of the [14C] palmitate and the [14C] hexadecanol into the recovered phospholipids and plasmalogens was severely decreased in the tissues perfused with CN-: in the hypoxic state only a mild inhibition was observed compared with the oxygenated systems. Considerable 3H from [1-14C, 1-3H] hexadecanol was retained (25-35%) in the alk-1-enylether chains of plasmalogens under both the oxygenated conditions and with CN-, suggesting that the same mechanism of incorporation is operational at high or low O2 concentrations. The results are consistent with an O2-dependent, CN-sensitive step in the biosynthesis of plasmalogens in the rat heart.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARTLETT G. R. Colorimetric assay methods for free and phosphorylated glyceric acids. J Biol Chem. 1959 Mar;234(3):469–471. [PubMed] [Google Scholar]
  2. Bassett D. J., Fisher A. B., Rabinowitz J. L. Effect of hypoxia on incorporation of glucose carbons into lipids by isolated rat lung. Am J Physiol. 1974 Nov;227(5):1103–1108. doi: 10.1152/ajplegacy.1974.227.5.1103. [DOI] [PubMed] [Google Scholar]
  3. Marsh J. B., Weinstein D. B. Simple charring method for determination of lipids. J Lipid Res. 1966 Jul;7(4):574–576. [PubMed] [Google Scholar]
  4. McMurray W. C., Magee W. L. Phospholipid metabolism. Annu Rev Biochem. 1972;41(10):129–160. doi: 10.1146/annurev.bi.41.070172.001021. [DOI] [PubMed] [Google Scholar]
  5. Paltauf F., Holasek A. Enzymatic synthesis of plasmalogens. Characterization of the 1-O-alkyl-2-acyl-8n-glycero-3-phosphorylethanolamine desaturase from mucosa of hamster small intestine. J Biol Chem. 1973 Mar 10;248(5):1609–1615. [PubMed] [Google Scholar]
  6. Paltauf F. Plasmalogen biosynthesis in a cell-free system. Enzymic desaturation of 1-O-alkyl (2-acyl) glycerophosphoryl ethanolamine. FEBS Lett. 1972 Jan 15;20(1):79–82. doi: 10.1016/0014-5793(72)80021-8. [DOI] [PubMed] [Google Scholar]
  7. Paltuaf F., Prough R. A., Masters B. S., Johnston J. M. Evidence for the participation of cytochrome b5 in plasmalogen biosynthesis. J Biol Chem. 1974 Apr 25;249(8):2661–2662. [PubMed] [Google Scholar]
  8. Rabinowitz J. L., Bailey T. A., Marsh J. B. Polar and neutral lipids of pig gingiva. Arch Oral Biol. 1971 Oct;16(10):1195–1205. doi: 10.1016/0003-9969(71)90049-5. [DOI] [PubMed] [Google Scholar]
  9. Rabinowitz J. L., Hercker E. S. Incomplete oxidation of palmitate and leakage of intermediary products during anoxia. Arch Biochem Biophys. 1974 Apr 2;161(2):621–627. doi: 10.1016/0003-9861(74)90345-2. [DOI] [PubMed] [Google Scholar]
  10. Renkonen O. Chromatographic separation of plasmalogenic, alkyl-acyl, and diacyl forms of ethanolamine glycerophosphatides. J Lipid Res. 1968 Jan;9(1):34–39. [PubMed] [Google Scholar]
  11. Simon G., Rouser G. Species variations in phospholipid class distribution of organs. II. Heart and skeletal muscle. Lipids. 1969 Nov;4(6):607–614. doi: 10.1007/BF02531048. [DOI] [PubMed] [Google Scholar]
  12. Simon G., Rouser G. Species variations in phospholipid class distribution of organs. II. Heart and skeletal muscle. Lipids. 1969 Nov;4(6):607–614. doi: 10.1007/BF02531048. [DOI] [PubMed] [Google Scholar]
  13. Viswanathan C. V., Basilio M., Hoevet S. P., Lundberg W. O. Reaction thin-layer chromatography in the analysis of mixtures of alkenyl acyl- and diacyl-phosphatides. J Chromatogr. 1968 Apr 9;34(2):241–245. doi: 10.1016/0021-9673(68)80042-1. [DOI] [PubMed] [Google Scholar]
  14. Viswanathan C. V. Chromatographic analysis of alkoxy-lipids. J Chromatogr. 1974 May 27;98(1):128–155. [PubMed] [Google Scholar]
  15. Viswanathan C. V., Phillips F., Lundberg W. O. Two-dimensional reaction thin-layer chromatography in the analysis of phosphatide plasmalogens. J Chromatogr. 1968 May 21;35(1):66–71. doi: 10.1016/s0021-9673(01)82350-5. [DOI] [PubMed] [Google Scholar]
  16. Wood R., Healy K. Tumor lipids. Biosynthesis of plasmalogens. J Biol Chem. 1970 May 25;245(10):2640–2648. [PubMed] [Google Scholar]
  17. Wykle R. L., Blank M. L., Malone B., Snyder F. Evidence for a mixed function oxidase in the biosynthesis of ethanolamine plasmalogens from 1-alkyl-2-acyl-sn-glycero-3-phosphorylethanolamine. J Biol Chem. 1972 Sep 10;247(17):5442–5447. [PubMed] [Google Scholar]
  18. Wykle R. L., Schremmer Lockmiller J. M. The biosynthesis of plasmalogens by rat brain: involvement of the microsomal electron transport system. Biochim Biophys Acta. 1975 Feb 20;380(2):291–298. doi: 10.1016/0005-2760(75)90015-6. [DOI] [PubMed] [Google Scholar]
  19. van den Bosch H. Phosphoglyceride metabolism. Annu Rev Biochem. 1974;43(0):243–277. doi: 10.1146/annurev.bi.43.070174.001331. [DOI] [PubMed] [Google Scholar]

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