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. 1977 Jan 1;161(1):149–158. doi: 10.1042/bj1610149

Glycosidases from the culture medium of Physarum polycephalum.

D C Kilpatrick, J L Stirling
PMCID: PMC1164483  PMID: 15539

Abstract

Eight exo-glycosidase activities were detected in the axenic culture medium of the myxomycete, Physarum polycephalum. The secretion of each enzyme examined followed the growth curve and continued during the stationary phase after the cessation of growth. Two or more forms of each enzyme were detected after electrophoretic separation. The beta-N-acetyl-D-hexosaminidase activity was readily separated into its two electrophoretic forms, X and Y, which were purified 145- and 306-fold respectively. These beta-N-acetyl-D-hexosaminidases had several similar characteristics. Evidence is presented that the major electrophoretic form of alpha-D-galactosidase is heterogeneous. The possible functions of extracellular glycosidases in teir occurrence and properties.

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Selected References

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  1. Andrews P. Estimation of the molecular weights of proteins by Sephadex gel-filtration. Biochem J. 1964 May;91(2):222–233. doi: 10.1042/bj0910222. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bosmann H. B. Elevated glycosidases and proteolytic enzymes in cells transformed by RNA tumor virus. Biochim Biophys Acta. 1972 Apr 21;264(2):339–343. doi: 10.1016/0304-4165(72)90298-x. [DOI] [PubMed] [Google Scholar]
  3. Bosmann H. B., Lockwood T., Morgan H. R. Surface biochemical changes accompanying primary infection with Rous sarcoma virus. II. Proteolytic and glycosidase activity and sublethal autolysis. Exp Cell Res. 1974 Jan;83(1):25–30. doi: 10.1016/0014-4827(74)90683-1. [DOI] [PubMed] [Google Scholar]
  4. Braun R., Behrens K. A ribonuclease from Physarum. Biochemical properties and synthesis in the mitotic cycle. Biochim Biophys Acta. 1969 Nov 19;195(1):87–98. doi: 10.1016/0005-2787(69)90605-4. [DOI] [PubMed] [Google Scholar]
  5. Carlile M. J., Dee J. Plasmodial fusion and lethal interaction between strains in a myxomycete. Nature. 1967 Aug 19;215(5103):832–834. doi: 10.1038/215832a0. [DOI] [PubMed] [Google Scholar]
  6. Carlile M. J. Nutrition and chemotaxis in the myxomycete Physarum polycephalum: the effect of carbohydrates on the plasmodium. J Gen Microbiol. 1970 Oct;63(2):221–226. doi: 10.1099/00221287-63-2-221. [DOI] [PubMed] [Google Scholar]
  7. Dance N., Price R. G., Robinson D., Stirling J. L. Beta-galactosidase, beta-glucosidase and N-acetyl-beta-glucosaminidase in human kidney. Clin Chim Acta. 1969 May;24(2):189–197. doi: 10.1016/0009-8981(69)90311-8. [DOI] [PubMed] [Google Scholar]
  8. Dee J. Slime moulds in biological research. Sci Prog. 1975 Winter;62(248):523–542. [PubMed] [Google Scholar]
  9. Dey P. M., Pridham J. B. Biochemistry of -galactosidases. Adv Enzymol Relat Areas Mol Biol. 1972;36:91–130. doi: 10.1002/9780470122815.ch3. [DOI] [PubMed] [Google Scholar]
  10. Every D., Ashworth J. M. The purification and properties of extracellular glycosidases of the cellular slime mould Dictyostelium discoideum. Biochem J. 1973 May;133(1):37–47. doi: 10.1042/bj1330037. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. FINDLAY J., LEVVY G. A. Purification of beta-N-acetylglucosaminidase from the pig epididymis. Biochem J. 1960 Oct;77:170–175. doi: 10.1042/bj0770170. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Farr D. R., Amster H., Horisberger M. Composition and partial structure of the extracellular polysaccharide of Physarum polycephalum. Carbohydr Res. 1972 Sep;24(1):207–209. doi: 10.1016/s0008-6215(00)82282-x. [DOI] [PubMed] [Google Scholar]
  13. Ho M. W. Hydrolysis of ceramide trihexoside by a specific -galactosidase from human liver. Biochem J. 1973 May;133(1):1–10. doi: 10.1042/bj1330001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Jeffery W. R., Rusch H. P. Induction of somatic fusion and heterokaryosis in two incompatible strains of Physarum polycephalum. Dev Biol. 1974 Aug;39(2):331–335. doi: 10.1016/0012-1606(74)90246-2. [DOI] [PubMed] [Google Scholar]
  15. Kilpatrick D. C., Stirling J. L. Two forms of beta-N-acetylhexosaminidase from Physarum polycephalum. Biochem Soc Trans. 1975;3(2):246–247. doi: 10.1042/bst0030246. [DOI] [PubMed] [Google Scholar]
  16. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  17. Li S. C., Li Y. T. Studies on the glycosidases of jack bean meal. 3. Crystallization and properties of beta-N-acetylhexosaminidase. J Biol Chem. 1970 Oct 10;245(19):5153–5160. [PubMed] [Google Scholar]
  18. Ling H. Genetics of somatic fusion in a myxomycete: F 2 studies. Protoplasma. 1971;73(3):407–416. doi: 10.1007/BF01273943. [DOI] [PubMed] [Google Scholar]
  19. McCormick J. J., Blomquist J. C., Rusch H. P. Isolation and Characterization of an Extracellular Polysaccharide from Physarum polycephalum. J Bacteriol. 1970 Dec;104(3):1110–1118. doi: 10.1128/jb.104.3.1110-1118.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Mega T., Ikenaka T., Matsushima Y. Studies on N-acetyl-beta-D-glucosaminidase of Aspergillus oryzae. I. Purification and characterization of N-acetyl-beta-D-glucosaminidase obtained from Takadiastase. J Biochem. 1970 Jul;68(1):109–117. [PubMed] [Google Scholar]
  21. Muramatsu T. N-acetylhexosaminidases from a gastropod, Turbo cornutus. J Biochem. 1968 Oct;64(4):521–531. doi: 10.1093/oxfordjournals.jbchem.a128925. [DOI] [PubMed] [Google Scholar]
  22. O'Day D. R. Intracellular localization and extracellular release of certain lysosomal enzyme activities from amoebae of the cellular slime mould Polysphondylium pallidum. Cytobios. 1973 Jul-Aug;7(28):223–232. [PubMed] [Google Scholar]
  23. Poste G. Changes in susceptibility of normal cells to agglutination by plant lectins following modification of cell coat material. Exp Cell Res. 1972 Aug;73(2):319–328. doi: 10.1016/0014-4827(72)90054-7. [DOI] [PubMed] [Google Scholar]
  24. Poste G. Sub-lethal autolysis. Modification of cell periphery by lysosomal enzymes. Exp Cell Res. 1971 Jul;67(1):11–16. doi: 10.1016/0014-4827(71)90615-x. [DOI] [PubMed] [Google Scholar]
  25. Poulter R. T., Dee J. Segregation of factors controlling fusion between plasmodia of the true slime mould Physarum polycephalum. Genet Res. 1968 Aug;12(1):71–79. doi: 10.1017/s0016672300011630. [DOI] [PubMed] [Google Scholar]
  26. Robinson D. Multiple forms of glycosidases in the normal and pathological states. Enzyme. 1974;18(1):114–135. doi: 10.1159/000459418. [DOI] [PubMed] [Google Scholar]
  27. Robinson D., Price R. G., Dance N. Separation and properties of beta-galactosidase, beta-glucosidase, beta-glucuronidase and N-acetyl-beta-glucosaminidase from rat kidney. Biochem J. 1967 Feb;102(2):525–532. doi: 10.1042/bj1020525. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Robinson D., Thorpe R. Fluorescent assay of alpha-L-fucosidase. Clin Chim Acta. 1974 Aug 30;55(1):65–69. doi: 10.1016/0009-8981(74)90334-9. [DOI] [PubMed] [Google Scholar]
  29. Ross I. K., Shipley G. L. Sexual and somatic fusion in the heterothallic slime mould Didymium iridis. 2. Effects of actinomycin D, cyclohexamide and lysosome stabilizers. Microbios. 1973 Feb-Mar;7(26):165–171. [PubMed] [Google Scholar]
  30. SMITHIES O. Zone electrophoresis in starch gels: group variations in the serum proteins of normal human adults. Biochem J. 1955 Dec;61(4):629–641. doi: 10.1042/bj0610629. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Stirling J. L. Human N-acetyl-beta-hexosaminidases: hydrolysis of N, N' diacetylchitobiose by a low molecular weight enzyme. FEBS Lett. 1974 Feb 15;39(2):171–175. doi: 10.1016/0014-5793(74)80044-x. [DOI] [PubMed] [Google Scholar]
  32. WOOLLEN J. W., WALKER P. G., HEYWORTH R. Studies on glucosaminidase. 6. N-Acetyl-beta-glucosaminidase and N-acetyl-beta-galactosaminidase activities of a variety of enzyme preparations. Biochem J. 1961 May;79:294–298. doi: 10.1042/bj0790294. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Winzler R. J. Carbohydrates in cell surfaces. Int Rev Cytol. 1970;29:77–125. doi: 10.1016/s0074-7696(08)60033-9. [DOI] [PubMed] [Google Scholar]

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