Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1977 Mar 1;161(3):527–534. doi: 10.1042/bj1610527

Human collagen 'fingerprints' produced by clostridopeptidase A digestion and high-pressure liquid chromatography.

M van Der Rest, W G Cole, F H Glorieux
PMCID: PMC1164537  PMID: 192198

Abstract

Samples (1-2mg) of purified human type I, II and III collagens and alpha1(I) and alpha2 chains were digested with clostridiopeptidase A and the released peptides analysed by ion-exchange high-pressure liquid chromatography. Specific 'fingerprints' were produced for each type of collagen. The reproducible nature of these 'fingerprints' and the reconstitution of the type I 'fingerprint' from the 'fingerprints' of the component alpha1(I) and alpha2 chains showed that the specificity of these 'fingerprints' was related to the primary structure of each type of collagen. In addition, some of the differences observed between the 'fingerprints' of the alpha1(I) and alpha2 chains of type I collagen were shown to be suitable for the quantitative analysis of these chains.

Full text

PDF
527

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BORNSTEIN P., PIEZ K. A. A BIOCHEMICAL STUDY OF HUMAN SKIN COLLAGEN AND THE RELATION BETWEEN INTRA- AND INTERMOLECULAR CROSS-LINKING. J Clin Invest. 1964 Sep;43:1813–1823. doi: 10.1172/JCI105055. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bornstein P. Comparative sequence studies of rat skin and tendon collagen. I. Evidence for incomplete hydroxylation of individual prolyl residues in the normal proteins. Biochemistry. 1967 Oct;6(10):3082–3093. doi: 10.1021/bi00862a015. [DOI] [PubMed] [Google Scholar]
  3. Bradley K., McConnell-Breul S., Crystal R. G. Lung collagen heterogeneity. Proc Natl Acad Sci U S A. 1974 Jul;71(7):2828–2832. doi: 10.1073/pnas.71.7.2828. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chung E., Miller E. J. Collagen polymorphism: characterization of molecules with the chain composition (alpha 1 (3)03 in human tissues. Science. 1974 Mar;183(130):1200–1201. doi: 10.1126/science.183.4130.1200. [DOI] [PubMed] [Google Scholar]
  5. Dixit S. N., Kang A. H., Gross J. Covalent structure of collagen: amino acid sequence of alpha1-CB3 of chick skin collagen. Biochemistry. 1975 May 6;14(9):1929–1933. doi: 10.1021/bi00680a019. [DOI] [PubMed] [Google Scholar]
  6. Epstein E. H., Jr (Alpha1(3))3 human skin collagen. Release by pepsin digestion and preponderance in fetal life. J Biol Chem. 1974 May 25;249(10):3225–3231. [PubMed] [Google Scholar]
  7. Eyre D. R., Muir H. Characterisation of the major CNBr-Derived peptides of porcine type II collagen. Connect Tissue Res. 1975;3(2):165–170. doi: 10.3109/03008207509152175. [DOI] [PubMed] [Google Scholar]
  8. Eyre D. R., Muir H. The distribution of different molecular species of collagen in fibrous, elastic and hyaline cartilages of the pig. Biochem J. 1975 Dec;151(3):595–602. doi: 10.1042/bj1510595. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Eyre D. R., Muir H. Type III collagen: A major constituent of rheumatoid and normal human synovial membrane. Connect Tissue Res. 1975;4(1):11–16. doi: 10.3109/03008207509152192. [DOI] [PubMed] [Google Scholar]
  10. Gallop P. M., Paz M. A. Posttranslational protein modifications, with special attention to collagen and elastin. Physiol Rev. 1975 Jul;55(3):418–487. doi: 10.1152/physrev.1975.55.3.418. [DOI] [PubMed] [Google Scholar]
  11. Goldberg B., Epstein E. H., Jr, Sherr C. J. Precursors of collagen secreted by cultured human fibroblasts. Proc Natl Acad Sci U S A. 1972 Dec;69(12):3655–3659. doi: 10.1073/pnas.69.12.3655. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gross J. Collagen biology: structure, degradation, and disease. Harvey Lect. 1974;68:351–432. [PubMed] [Google Scholar]
  13. Harper E., Kang A. H. Studies on the specificity of bacterial collagenase. Biochem Biophys Res Commun. 1970 Oct 23;41(2):482–487. doi: 10.1016/0006-291x(70)90531-0. [DOI] [PubMed] [Google Scholar]
  14. Henneman D. H. Comparative solubility of bone and skin collagen in primates including man. J Med Primatol. 1972;1(1):58–67. doi: 10.1159/000460362. [DOI] [PubMed] [Google Scholar]
  15. Miller E. J., Lunde L. G. Isolation and characterization of the cyanogen bromide peptides from the alpha 1(II) chain of bovine and human cartilage collagen. Biochemistry. 1973 Aug 14;12(17):3153–3159. doi: 10.1021/bi00741a003. [DOI] [PubMed] [Google Scholar]
  16. Miller E. J. Structural studies on cartilage collagen employing limited cleavage and solubilization with pepsin. Biochemistry. 1972 Dec 19;11(26):4903–4909. doi: 10.1021/bi00776a005. [DOI] [PubMed] [Google Scholar]
  17. Miyoshi M., Rosenbloom J. General proteolytic activity of highly purified preparations of clostridial collagenase. Connect Tissue Res. 1974;2(2):77–84. doi: 10.3109/03008207409152091. [DOI] [PubMed] [Google Scholar]
  18. Paz M. A., Gallop P. M. Collagen synthesized and modified by aging fibroblasts in culture. In Vitro. 1975 Sep-Oct;11(5):302–312. doi: 10.1007/BF02615641. [DOI] [PubMed] [Google Scholar]
  19. Peterkofsky B., Diegelmann R. Use of a mixture of proteinase-free collagenases for the specific assay of radioactive collagen in the presence of other proteins. Biochemistry. 1971 Mar 16;10(6):988–994. doi: 10.1021/bi00782a009. [DOI] [PubMed] [Google Scholar]
  20. Robertson W. V., Harvey J. The determination of galactose in urinary acidic glycosaminoglycans as a measure of keratan sulfate-like substances in urine. Biochem Med. 1972 Jun;6(3):246–256. doi: 10.1016/0006-2944(72)90045-2. [DOI] [PubMed] [Google Scholar]
  21. Seyer J. M., Brickley D. M., Glimcher M. J. The identification of two types of collagen in the articular cartilage of postnatal chickens. Calcif Tissue Res. 1974;17(1):43–55. doi: 10.1007/BF02547213. [DOI] [PubMed] [Google Scholar]
  22. Seyer J. M., Brickley D. M., Glimcher M. J. The isolation of two types of collagen from embryonic bovine epiphyseal cartilage. Calcif Tissue Res. 1974;17(1):25–41. doi: 10.1007/BF02547212. [DOI] [PubMed] [Google Scholar]
  23. Trelstad R. L. Human aorta collagens: evidence for three distinct species. Biochem Biophys Res Commun. 1974 Apr 8;57(3):717–725. doi: 10.1016/0006-291x(74)90605-6. [DOI] [PubMed] [Google Scholar]
  24. Trelstad R. L., Kang A. H., Toole B. P., Gross J. Collagen heterogeneity. High resolution separation of native ( 1(I) 2 2 and ( 1(II) 3 and their component chains. J Biol Chem. 1972 Oct 25;247(20):6469–6473. [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES