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. 1977 Jan 15;162(1):87–97. doi: 10.1042/bj1620087

Deoxyribonucleic acid and polyamine synthesis in rat ventral prostrate. Effects of age of the intact rat and androgen stimulation of the castrated rat with testosterone, 5 alpha-dihydrotestosterone and 5 alpha-androstane-3 beta, 17 beta-diol.

E E Takyi, D J Fuller, L J Donaldson, G H Thomas
PMCID: PMC1164572  PMID: 849281

Abstract

The relationship between polyamine synthesis, growth and secretion in vivo was examined in ventral prostates from: (a) intact rats aged 3-60 weeks; (b) animals castrated for 7 days before injection with 5 alpha-dihydrotestosterone (17 beta-hydroxy-5-alpha-androstan-3-one), testosterone and 5 alpha-androstane-3 beta, 17 beta-diol for up to 10 days; (c) rats injected with the 3 beta, 17 beta-diol immediately after castration. Ornithine decarboxylase activity and the concentrations of putrescine, spermidine and spermine were measured. DNA-synthetic activity was monitored by measuring [125I]iododoxyuridine incorporation. An enhanced spermidine/spermine molar ratio reflected increased activity of the prostate. The ratio was higher (greater than 2) in prostates from sexually immature animals, than in the intact adult (1.5), suggesting that the ratio was indicative of the proliferative activity of the tissue. However, in the androgen-stimulated castrated rat, enhanced spermidine/spermine ratios tended to correlate with hypertrophy and secretion. In both sets of experiments there was a linear relationship between protein and spermidine content. High spermidine/spermine molar ratios were the consequence of a relatively low rate of accumulation of spermine relative to spermidine and protein. The relationship between polyamine synthesis and DNA-synthetic activity was investigated in cultured prostate. A combination of insulin (3 mug/ml) and testosterone (0.1 muM caused a stimulatory response in the incorporation of [125I]iododeoxyuridine and in cell division, despite a depleted polyamine content and low ornithine decarboxylase activity in the cultured tissue.

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Selected References

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  1. Becker H., Grabosch E., Hoffmann C., Voigt K. D. Metabolism and mode of action of androgens in target tissues of male rats. 3. Metabolism of 5 alpha-androstane-3,17-dione, of 5 alpha-androstane-3 beta,17 beta-diol and of 5 alpha-androstane-3 beta,17 beta-diol in target organs and peripheral tissues. Acta Endocrinol (Copenh) 1973 Jun;73(2):407–416. doi: 10.1530/acta.0.0730407. [DOI] [PubMed] [Google Scholar]
  2. Caldarera C. M., Moruzzi M. S., Barbiroli B., Moruzzi G. Spermine and spermidine of the prostate gland of orchiectomized rats and their effect on RNA polymerase activity. Biochem Biophys Res Commun. 1968 Oct 24;33(2):266–271. doi: 10.1016/0006-291x(68)90779-1. [DOI] [PubMed] [Google Scholar]
  3. Clifton K. H., Cooper J. M. Reutilization of thymidine and iododeoxyuridine by mouse mammary carcinoma strain MTG-B 1 . Proc Soc Exp Biol Med. 1973 Apr;142(4):1145–1151. doi: 10.3181/00379727-142-37195. [DOI] [PubMed] [Google Scholar]
  4. Coffey D. S., Shimazaki J., Williams-Ashman H. G. Polymerization of deoxyribonucleotides in relation to androgen-induced prostatic growth. Arch Biochem Biophys. 1968 Mar 20;124(1):184–198. doi: 10.1016/0003-9861(68)90319-6. [DOI] [PubMed] [Google Scholar]
  5. Dethlefsen L. A. 3H-5-iodo-2'-deoxyuridine toxicity. Problems in cell proliferation studies. Cell Tissue Kinet. 1974 May;7(3):213–222. doi: 10.1111/j.1365-2184.1974.tb00901.x. [DOI] [PubMed] [Google Scholar]
  6. Dethlefsen L. A. An evaluation of radioiodine-labeled 5-iodo-2'-deoxyuridine as a tracer for measuring cell loss from solid tumors. Cell Tissue Kinet. 1971 Mar;4(2):123–138. doi: 10.1111/j.1365-2184.1971.tb01523.x. [DOI] [PubMed] [Google Scholar]
  7. Donaldson L. J., Thomas G. H. Uptake of (125I) iododeoxyuridine in cultured rat and human prostate: effects of insulin and testosterone. J Endocrinol. 1976 Apr;69(1):85–92. doi: 10.1677/joe.0.0690085. [DOI] [PubMed] [Google Scholar]
  8. Fuller D. J., Donaldson L. J., Thomas G. H. Ornithine decarboxylase activity and: [125I]iododeoxyuridine incorporation in rat prostate. Biochem J. 1975 Sep;150(3):557–559. doi: 10.1042/bj1500557. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Geiger P. J., Bessman S. P. Protein determination by Lowry's method in the presence of sulfhydryl reagents. Anal Biochem. 1972 Oct;49(2):467–473. doi: 10.1016/0003-2697(72)90450-2. [DOI] [PubMed] [Google Scholar]
  10. HUGHES W. L., COMMERFORD S. L., GITLIN D., KRUEGER R. C., SCHULTZE B., SHAH V., REILLY P. DEOXYRIBONUCLEIC ACID METABOLISM IN VIVO: I. CELL PROLIFERATION AND DEATH AS MEASURED BY INCORPORATION AND ELIMINATION OF IODODEOXYURIDINE. Fed Proc. 1964 May-Jun;23:640–648. [PubMed] [Google Scholar]
  11. Jänne J., Williams-Ashman H. G. Mammalian ornithine decarboxylase: activation and alteration of physical behaviour by thiol compounds. Biochem J. 1970 Sep;119(3):595–597. doi: 10.1042/bj1190595. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Jänne J., Williams-Ashman H. G. On the purification of L-ornithine decarboxylase from rat prostate and effects of thiol compounds on the enzyme. J Biol Chem. 1971 Mar 25;246(6):1725–1732. [PubMed] [Google Scholar]
  13. Krieg M., Horst H. J., Sterba M. L. Binding and metabolism of 5alpha-androstane-3alpha, 17 beta-diol and of 5alpha-androstane-3beta, 17 beta-diol in the prostate, seminal vesicles and plasma of male rats: studies in vivo and in vitro. J Endocrinol. 1975 Mar;64(3):529–538. doi: 10.1677/joe.0.0640529. [DOI] [PubMed] [Google Scholar]
  14. LASNITZKI I., LUCY J. A. Amino acid metabolism and arginase activity in mouse prostate glands grown in vitro with and without 20-methylcholanthrene. Exp Cell Res. 1961 Aug;24:379–392. doi: 10.1016/0014-4827(61)90439-6. [DOI] [PubMed] [Google Scholar]
  15. Lesser B., Bruchovsky N. Effect of duration of the period after castration on the response of the rat ventral prostate to androgens. Biochem J. 1974 Aug;142(2):429–431. doi: 10.1042/bj1420429. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lesser B., Bruchovsky N. The effects of testosterone, 5 -dihydrotestosterone and adenosine 3',5'-monophosphate on cell proliferation and differentiation in rat prostate. Biochim Biophys Acta. 1973 May 18;308(3):426–437. doi: 10.1016/0005-2787(73)90336-5. [DOI] [PubMed] [Google Scholar]
  17. Liao S., Fang S. Receptor-proteims for androgens and the mode of action of androgens on gene transcription in ventral prostate. Vitam Horm. 1969;27:17–90. doi: 10.1016/s0083-6729(08)61124-3. [DOI] [PubMed] [Google Scholar]
  18. Lostroh A. J. Regulation by testosterone and insulin of citrate secretion and protein synthesis in explanted mouse prostates. Proc Natl Acad Sci U S A. 1968 Aug;60(4):1312–1318. doi: 10.1073/pnas.60.4.1312. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Mangan F. R., Pegg A. E., Mainwaring I. P. A reappraisal of the effects of adenosine 3':5'-cyclic monophosphate on the function and morphology of the rat prostate gland. Biochem J. 1973 May;134(1):129–142. doi: 10.1042/bj1340129. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Micklem H. S. Cell proliferation in haematopoietic spleen colonies of mice: difference between colonies derived from injected adult bone marrow and foetal liver cells. Cell Tissue Kinet. 1972 Mar;5(2):159–164. doi: 10.1111/j.1365-2184.1972.tb01012.x. [DOI] [PubMed] [Google Scholar]
  21. Moulton B. C., Leonard S. L. Hormonal effects on spermidine levels in male and female reproductive organs of the rat. Endocrinology. 1969 Jun;84(6):1461–1465. doi: 10.1210/endo-84-6-1461. [DOI] [PubMed] [Google Scholar]
  22. Pegg A. E., Lockwood D. H., Williams-Ashman H. G. Concentrations of putrescine and polyamines and their enzymic synthesis during androgen-induced prostatic growth. Biochem J. 1970 Mar;117(1):17–31. doi: 10.1042/bj1170017. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Pegg A. E., Williams-Ashman H. G. Biosynthesis of putrescine in the prostate gland of the rat. Biochem J. 1968 Jul;108(4):533–539. doi: 10.1042/bj1080533. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Rennie P. S., Symes E. K., Mainwaring W. J. The androgenic regulation of the activities of enzymes engaged in the synthesis of deoxyribonucleic acid in rat ventral prostate gland. Biochem J. 1975 Oct;152(1):1–16. doi: 10.1042/bj1520001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Russell D. H., McVicker T. A. Polyamine biogenesis in the rat mammary gland during pregnancy and lactation. Biochem J. 1972 Nov;130(1):71–76. doi: 10.1042/bj1300071. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Santti R. S., Johansson R. Some biochemical effects of insulin and steroid hormones on the rat prostate in organ culture. Exp Cell Res. 1973 Mar 15;77(1):111–120. doi: 10.1016/0014-4827(73)90559-4. [DOI] [PubMed] [Google Scholar]
  27. Schmidt H., Giba-Tziampiri O., von Rotteck G., Voigt K. D. Metabolism and mode of action of androgens in target tissue of male rats. IV. Mode of action of 5 alpha-androstane-3,17-dione, 5 alpha-androstane-3 alpha,17 beta-diol and of 5 alpha-androstane-3 beta,17 beta-diol at a cellular level on seminal vesicles and prostates of rats. Acta Endocrinol (Copenh) 1973 Jul;73(3):599–611. [PubMed] [Google Scholar]
  28. Shipman P. A., Littlewood V., Riches A. C., Thomas G. H. Differences in proliferative activity of rat and human prostate in culture. Br J Cancer. 1975 May;31(5):570–580. doi: 10.1038/bjc.1975.98. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. TABOR H., TABOR C. W. SPERMIDINE, SPERMINE, AND RELATED AMINES. Pharmacol Rev. 1964 Sep;16:245–300. [PubMed] [Google Scholar]
  30. Tuohimaa P., Söderström K. O. Weight gain and (3H)thymidine incorporation in accessory sex glands of castrated male rats after physiological doses of testosterone. Acta Endocrinol (Copenh) 1974 Nov;77(3):597–603. doi: 10.1530/acta.0.0770597. [DOI] [PubMed] [Google Scholar]
  31. Williams-Ashman H. G., Coppoc G. L., Weber G. Imbalance in ornithine metabolism in hepatomas of different growth rates as expressed in formation of putrescine, spermidine, and spermine. Cancer Res. 1972 Sep;32(9):1924–1932. [PubMed] [Google Scholar]

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