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. 1977 Feb 15;162(2):309–320. doi: 10.1042/bj1620309

Transport of galactose, glucose and their molecular analogues by Escherichia coli K12.

P J Henderson, R A Giddens, M C Jones-Mortimer
PMCID: PMC1164603  PMID: 15558

Abstract

1. Strains of Escherichia coli K12 were made that are unable to assimilate glucose by the phosphotransferase system, since they lack the glucose-specific components specified by the genes ptsG and ptsM. 2. Derivative organisms lacking the methyl galactoside or galactose-specific transport system were examined for their ability to transport galactose, d-fucose, methyl beta-D-galactoside, glucose, 2-deoxy-D-glucose and methyl alpha-D-glucoside. 3. Galactose, glucose and to a lesser extent fucose are substrates for both transport systems. 4. 2-Deoxyglucose is transported on the galactose-specific but not the methyl galactoside system. 5. The ability of sugars to elicit anaerobic proton transport is associated with the galactose-specific, but not with the methyl galactoside transport activity. Hence a chemiosmotic mechanism of energization is likely to apply to the former but not to the latter. Alternatively the methyl galactoside system may be switched off under certain conditions, which would indicate a novel regulatory mechanism. 6. Details of the procedure for the derivation of strains may be obtained from the authors, and have been deposited as Supplementary Publication SUP 50074 (8 pages at the) British Library Lending Division, Boston Spa, Wetherby, West Yorkshire LS23 7BQ, U.K., from whom copies can be obtained on the terms indicated in Biochem. J. (1977), 161,1.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adler J. Chemotaxis in bacteria. Annu Rev Biochem. 1975;44:341–356. doi: 10.1146/annurev.bi.44.070175.002013. [DOI] [PubMed] [Google Scholar]
  2. Amaral D., Kornberg H. L. Regulation of fructose uptake by glucose in Escherichia coli. J Gen Microbiol. 1975 Sep;90(1):157–168. doi: 10.1099/00221287-90-1-157. [DOI] [PubMed] [Google Scholar]
  3. BUTTIN G. M'ECANISMES R'EGULATEURS DANS LA BIOSYNTH'ESE DES ENZYMES DU M'ETABOLISME DU GALACTOSE CHEZ ESCHERICHIA COLI K12. I. LA BIOSYNTH'ESE INDUITE DE LA GALACTOKINASE ET L'INDUCTION SIMULTAN'EE DE LA S'EQUENCE ENZYMATIQUE. J Mol Biol. 1963 Aug;7:164–182. doi: 10.1016/s0022-2836(63)80044-3. [DOI] [PubMed] [Google Scholar]
  4. BUTTIN G. M'ECANISMES R'EGULATEURS DANS LA BIOSYNTH'ESE DES ENZYMES DU M'ETABOLISME DU GALACTOSE CHEZ ESCHERICHIA COLI K12. II. LE D'ETERMINISME G'EN'ETIQUE DE LA R'EGULATION. J Mol Biol. 1963 Aug;7:183–205. doi: 10.1016/s0022-2836(63)80045-5. [DOI] [PubMed] [Google Scholar]
  5. Bachmann B. J., Low K. B., Taylor A. L. Recalibrated linkage map of Escherichia coli K-12. Bacteriol Rev. 1976 Mar;40(1):116–167. doi: 10.1128/br.40.1.116-167.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Bachmann B. J. Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriol Rev. 1972 Dec;36(4):525–557. doi: 10.1128/br.36.4.525-557.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Berger E. A. Different mechanisms of energy coupling for the active transport of proline and glutamine in Escherichia coli. Proc Natl Acad Sci U S A. 1973 May;70(5):1514–1518. doi: 10.1073/pnas.70.5.1514. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Berger E. A., Heppel L. A. Different mechanisms of energy coupling for the shock-sensitive and shock-resistant amino acid permeases of Escherichia coli. J Biol Chem. 1974 Dec 25;249(24):7747–7755. [PubMed] [Google Scholar]
  9. Beverin S., Sheppard D. E., Park S. S. D-Fucose as a gratuitous inducer of the L-arabinose operon in strains of Escherichia coli B-r mutant in gene araC. J Bacteriol. 1971 Jul;107(1):79–86. doi: 10.1128/jb.107.1.79-86.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Boos W. Bacterial transport. Annu Rev Biochem. 1974;43(0):123–146. doi: 10.1146/annurev.bi.43.070174.001011. [DOI] [PubMed] [Google Scholar]
  11. Boos W., Lengeler J., Hermann K. O., Unsöld H. J. The regulation of the beta-methylgalactoside transport system and of the galactose binding protein of Escherichia coli K12. Eur J Biochem. 1971 Apr 30;19(4):457–470. doi: 10.1111/j.1432-1033.1971.tb01336.x. [DOI] [PubMed] [Google Scholar]
  12. Boos W., Sarvas M. O. Close linkage between a galactose binding protein and the beta-methylgalactoside permease in Escherichia coli. Eur J Biochem. 1970 Apr;13(3):526–533. doi: 10.1111/j.1432-1033.1970.tb00956.x. [DOI] [PubMed] [Google Scholar]
  13. Boos W. The galactose binding protein and its relationship to the beta-methylgalactoside permease from Escherichia coli. Eur J Biochem. 1969 Aug;10(1):66–73. doi: 10.1111/j.1432-1033.1969.tb00656.x. [DOI] [PubMed] [Google Scholar]
  14. Buttin G. Les systemes enzymatiques inductibles du metabolisme des oses chez Escherichia coli. Adv Enzymol Relat Areas Mol Biol. 1968;30:81–137. [PubMed] [Google Scholar]
  15. Böck A., Neidhardt F. C. Isolation of a Mutant of Escherichia coli with a Temperature-sensitive Fructose-1,6-Diphosphate Aldolase Activity. J Bacteriol. 1966 Aug;92(2):464–469. doi: 10.1128/jb.92.2.464-469.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Curtis S. J., Epstein W. Phosphorylation of D-glucose in Escherichia coli mutants defective in glucosephosphotransferase, mannosephosphotransferase, and glucokinase. J Bacteriol. 1975 Jun;122(3):1189–1199. doi: 10.1128/jb.122.3.1189-1199.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Curtis S. J. Mechanism of energy coupling for transport of D-ribose in Escherichia coli. J Bacteriol. 1974 Oct;120(1):295–303. doi: 10.1128/jb.120.1.295-303.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Demerec M., Adelberg E. A., Clark A. J., Hartman P. E. A proposal for a uniform nomenclature in bacterial genetics. Genetics. 1966 Jul;54(1):61–76. doi: 10.1093/genetics/54.1.61. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Flagg J. L., Wilson T. H. Galactoside accumulation by Escherichia coli, driven by a pH gradient. J Bacteriol. 1976 Mar;125(3):1235–1239. doi: 10.1128/jb.125.3.1235-1236.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Ganesan A. K., Rotman B. Transport systems for galactose and galactosides in Escherichia coli. I. Genetic determination and regulation of the methyl-galactoside permease. J Mol Biol. 1966 Mar;16(1):42–50. doi: 10.1016/s0022-2836(66)80261-9. [DOI] [PubMed] [Google Scholar]
  21. Jones-Mortimer M. C., Kornberg H. L. Genetic control of inducer exclusion by Escherichia coli. FEBS Lett. 1974 Nov 1;48(1):93–95. doi: 10.1016/0014-5793(74)81070-7. [DOI] [PubMed] [Google Scholar]
  22. Jones-Mortimer M. C., Kornberg H. L. Order of genes adjacent to ptsX on the E. coli genome. Proc R Soc Lond B Biol Sci. 1976 May 18;193(1112):313–315. doi: 10.1098/rspb.1976.0049. [DOI] [PubMed] [Google Scholar]
  23. Kaiser A. D., Masuda T. Specificity in curing by heteroimmune superinfection. Virology. 1970 Mar;40(3):522–529. doi: 10.1016/0042-6822(70)90195-9. [DOI] [PubMed] [Google Scholar]
  24. Kashket E. R., Wilson T. H. Proton-coupled accumulation of galactoside in Streptococcus lactis 7962. Proc Natl Acad Sci U S A. 1973 Oct;70(10):2866–2869. doi: 10.1073/pnas.70.10.2866. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Kerwar G. K., Gordon A. S., Kaback H. R. Mechanisms of active transport in isolated membrane vesicles. IV. Galactose transport by isolated membrane vesicles from Escherichia coli. J Biol Chem. 1972 Jan 10;247(1):291–297. [PubMed] [Google Scholar]
  26. Kornberg H. L. Genetics in the study of carbohydrate transport by bacteria. Sixth Griffith Memorial Lecture. J Gen Microbiol. 1976 Sep;96(1):1–16. doi: 10.1099/00221287-96-1-1. [DOI] [PubMed] [Google Scholar]
  27. Kornberg H. L., Jones-Mortimer M. C. PtsX: a gene involved in the uptake of glucose and fructose by Escherichia coli. FEBS Lett. 1975 Mar 1;51(1):1–4. doi: 10.1016/0014-5793(75)80840-4. [DOI] [PubMed] [Google Scholar]
  28. Kornberg H. L., Riordan C. Uptake of galactose into Escherichia coli by facilitated diffusion. J Gen Microbiol. 1976 May;94(1):75–89. doi: 10.1099/00221287-94-1-75. [DOI] [PubMed] [Google Scholar]
  29. LENNOX E. S. Transduction of linked genetic characters of the host by bacteriophage P1. Virology. 1955 Jul;1(2):190–206. doi: 10.1016/0042-6822(55)90016-7. [DOI] [PubMed] [Google Scholar]
  30. Lawford H. G., Haddock B. A. Respiration-driven proton translocation in Escherichia coli. Biochem J. 1973 Sep;136(1):217–220. doi: 10.1042/bj1360217. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Low B. Rapid mapping of conditional and auxotrophic mutations in Escherichia coli K-12. J Bacteriol. 1973 Feb;113(2):798–812. doi: 10.1128/jb.113.2.798-812.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. MITCHELL P. Coupling of phosphorylation to electron and hydrogen transfer by a chemi-osmotic type of mechanism. Nature. 1961 Jul 8;191:144–148. doi: 10.1038/191144a0. [DOI] [PubMed] [Google Scholar]
  33. McKay L., Miller A., 3rd, Sandine W. E., Elliker P. R. Mechanisms of lactose utilization by lactic acid streptococci: enzymatic and genetic analyses. J Bacteriol. 1970 Jun;102(3):804–809. doi: 10.1128/jb.102.3.804-809.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Mitchell P. Performance and conservation of osmotic work by proton-coupled solute porter systems. J Bioenerg. 1973 Jan;4(1):63–91. doi: 10.1007/BF01516051. [DOI] [PubMed] [Google Scholar]
  35. Ordal G. W., Adler J. Isolation and complementation of mutants in galactose taxis and transport. J Bacteriol. 1974 Feb;117(2):509–516. doi: 10.1128/jb.117.2.509-516.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Ordal G. W., Adler J. Properties of mutants in galactose taxis and transport. J Bacteriol. 1974 Feb;117(2):517–526. doi: 10.1128/jb.117.2.517-526.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Oxender D. L. Membrane transport. Annu Rev Biochem. 1972;41(10):777–814. doi: 10.1146/annurev.bi.41.070172.004021. [DOI] [PubMed] [Google Scholar]
  38. Postma P. W. Involvement of the phosphotransferase system in galactose transport in Salmonella typhimurium. FEBS Lett. 1976 Jan 1;61(1):49–53. doi: 10.1016/0014-5793(76)80169-x. [DOI] [PubMed] [Google Scholar]
  39. Robbins A. R., Guzman R., Rotman B. Roles of individual mgl gene products in the beta-methylgalactoside transport system of Escherichia coli K12. J Biol Chem. 1976 May 25;251(10):3112–3116. [PubMed] [Google Scholar]
  40. Robbins A. R. Regulation of the Escherichia coli methylgalactoside transport system by gene mglD. J Bacteriol. 1975 Jul;123(1):69–74. doi: 10.1128/jb.123.1.69-74.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  41. Robbins A. R., Rotman B. Evidence for binding protein-independent substrate translocation by the methylgalactoside transport system of Escherichia coli K12. Proc Natl Acad Sci U S A. 1975 Feb;72(2):423–427. doi: 10.1073/pnas.72.2.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  42. Rotman B., Ganesan A. K., Guzman R. Transport systems for galactose and galactosides in Escherichia coli. II. Substrate and inducer specificities. J Mol Biol. 1968 Sep 14;36(2):247–260. doi: 10.1016/0022-2836(68)90379-3. [DOI] [PubMed] [Google Scholar]
  43. Sinnott M. L., Viratelle O. M. The effect of methanol and dioxan on the rates of the beta-galactosidase-catalysed hydrolyses of some beta-D-galactrophyranosides: rate-limiting degalactosylation. The ph-dependence of galactosylation and degalactosylation. Biochem J. 1973 May;133(1):81–87. doi: 10.1042/bj1330081. [DOI] [PMC free article] [PubMed] [Google Scholar]
  44. West I. C. Lactose transport coupled to proton movements in Escherichia coli. Biochem Biophys Res Commun. 1970 Nov 9;41(3):655–661. doi: 10.1016/0006-291x(70)90063-x. [DOI] [PubMed] [Google Scholar]
  45. West I. C., Mitchell P. Stoicheiometry of lactose-H+ symport across the plasma membrane of Escherichia coli. Biochem J. 1973 Mar;132(3):587–592. doi: 10.1042/bj1320587. [DOI] [PMC free article] [PubMed] [Google Scholar]
  46. West I., Mitchell P. Proton-coupled beta-galactoside translocation in non-metabolizing Escherichia coli. J Bioenerg. 1972 Aug;3(5):445–462. doi: 10.1007/BF01516082. [DOI] [PubMed] [Google Scholar]
  47. Wilson D. B. Source of energy for the Escherichia coli galactose transport systems induced by galactose. J Bacteriol. 1974 Nov;120(2):866–871. doi: 10.1128/jb.120.2.866-871.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  48. Wilson D. B. The regulation and properties of the galactose transport system in Escherichia coli K12. J Biol Chem. 1974 Jan 25;249(2):553–558. [PubMed] [Google Scholar]
  49. Winkler H. H., Wilson T. H. The role of energy coupling in the transport of beta-galactosides by Escherichia coli. J Biol Chem. 1966 May 25;241(10):2200–2211. [PubMed] [Google Scholar]
  50. Yudkin M. D. Catabolite repression of the lac operon. Separt epressionof two enzymes. Biochem J. 1969 Sep;114(2):313–319. doi: 10.1042/bj1140313. [DOI] [PMC free article] [PubMed] [Google Scholar]

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