Abstract
Lysosome-rich fractions were isolated from rat liver homogenates. In some fractions the lysosomes were separated from the contaminating mitchondria by the use of discontinuous Ficoll/sucrose gradients. Some glycogen was associated with lysosome fractions. This glycogen was of very large molecular size and of a quite different molecular-weight distribution from that isolated from the cytosol. It is suggested that appreciably more than 10% of cellular glycogen is located within the lysosome.
Full text
PDFSelected References
These references are in PubMed. This may not be the complete list of references from this article.
- BAUDHUIN P., BERLEUR A. N., DE DUVE C., WATTIAUX R. Tissue fractionation studies. VIII. Cellular localization of bound enzymes. Biochem J. 1956 Aug;63(4):608–612. doi: 10.1042/bj0630608. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Baxter-Gabbard K. L. A simple method for the large-scale preparation of sucrose gradients. FEBS Lett. 1972 Jan 15;20(1):117–119. doi: 10.1016/0014-5793(72)80031-0. [DOI] [PubMed] [Google Scholar]
- Brown J. H., Schwartz N. L. Interaction of lysosomes and anti-infalmmatory drugs. Proc Soc Exp Biol Med. 1969 Jun;131(2):614–620. doi: 10.3181/00379727-131-33938. [DOI] [PubMed] [Google Scholar]
- DE DUVE C., PRESSMAN B. C., GIANETTO R., WATTIAUX R., APPELMANS F. Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat-liver tissue. Biochem J. 1955 Aug;60(4):604–617. doi: 10.1042/bj0600604. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Davies M. The heterogeneity of lysosomes. Front Biol. 1975;43(4):305–348. [PubMed] [Google Scholar]
- GIANETTO R., DE DUVE C. Tissue fractionation studies. 4. Comparative study of the binding of acid phosphatase, beta-glucuronidase and cathepsin by rat-liver particles. Biochem J. 1955 Mar;59(3):433–438. doi: 10.1042/bj0590433. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Geddes R., Harvey J. D., Wills P. R. The molecular size and shape of liver glycogen. Biochem J. 1977 May 1;163(2):201–209. doi: 10.1042/bj1630201. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Geddes R., Rapson K. B. Post-mortem degradation of glycogen. FEBS Lett. 1973 May 1;31(3):324–326. doi: 10.1016/0014-5793(73)80132-2. [DOI] [PubMed] [Google Scholar]
- Ignarro L. J. Effects of anti-inflammatory drugs on the stability of rat liver lysosomes in vitro. Biochem Pharmacol. 1971 Oct;20(10):2847–2860. doi: 10.1016/0006-2952(71)90196-1. [DOI] [PubMed] [Google Scholar]
- KATZEN H. M., STETTEN D., Jr, STETTEN M. R. Metabolic inhomogeneity of glycogen as a function of molecular weight. J Biol Chem. 1956 Oct;222(2):587–599. [PubMed] [Google Scholar]
- KIRBY K. S. A new method for the isolation of ribonucleic acids from mammalian tissues. Biochem J. 1956 Nov;64(3):405–408. doi: 10.1042/bj0640405. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KRISMAN C. R. A method for the colorimetric estimation of glycogen with iodine. Anal Biochem. 1962 Jul;4:17–23. doi: 10.1016/0003-2697(62)90014-3. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- MARTIN R. G., AMES B. N. A method for determining the sedimentation behavior of enzymes: application to protein mixtures. J Biol Chem. 1961 May;236:1372–1379. [PubMed] [Google Scholar]
- MCCAMAN R. E., MCCAMAN M. W., HUNT J. M., SMITH M. S. MICRODETERMINATION OF MONOAMINE OXIDASE AND 5-HYDROXYTRYPTOPHAN DECARBOXYLASE ACTIVITIES IN NERVOUS TISSUES. J Neurochem. 1965 Jan;12:15–23. doi: 10.1111/j.1471-4159.1965.tb10246.x. [DOI] [PubMed] [Google Scholar]
- ROY A. B. The sulphatase of ox liver. 7. The intracellular distribution of sulphatases A and B. Biochem J. 1960 Nov;77:380–386. doi: 10.1042/bj0770380. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tan K. B. A simple device for fractionating density gradients. Anal Biochem. 1972 Jan;45(1):306–308. doi: 10.1016/0003-2697(72)90031-0. [DOI] [PubMed] [Google Scholar]
- den Tandt W. R. Acid hydrolases in uncultured amniotic fluid cells. Clin Chim Acta. 1972 Aug;40(1):199–204. doi: 10.1016/0009-8981(72)90271-9. [DOI] [PubMed] [Google Scholar]