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. 1977 May 1;163(2):201–209. doi: 10.1042/bj1630201

The molecular size and shape of liver glycogen.

R Geddes, J D Harvey, P R Wills
PMCID: PMC1164685  PMID: 869923

Abstract

The molecular-weight distribution of liver glycogen has been established from the analysis of sedimentation rates of fractions separated on sucrose density gradients and from the direct measurement of the diffusion coefficients of these fractions by laser-intensity-fluctuation spectroscopy. Hydrodynamic studies indicated that all fractions of glycogen of mol.wt.exceeding 25x10(6) had about 1.1 g of water per g of polysaccharide associated with them. The hydration and hydrodynamic behaviour of all fractions of mol.wt. exceeding 25x10(6) was similar, whereas smaller fractions behaved anomalously, indicating a substantially different overall structure.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANDERER F. A. RECENT STUDIES ON THE STRUCTURE OF TOBACCO MOSAIC VIRUS. Adv Protein Chem. 1963;18:1–35. doi: 10.1016/s0065-3233(08)60267-3. [DOI] [PubMed] [Google Scholar]
  2. Barber E. J. Calculation of density and viscosity of sucrose solutions as a function of concentration and temperature. Natl Cancer Inst Monogr. 1966 Jun;21:219–239. [PubMed] [Google Scholar]
  3. DROCHMANS P. [Morphology of glycogen. Electron microscopic study of the negative stains of particulate glycogen]. J Ultrastruct Res. 1962 Apr;6:141–163. doi: 10.1016/s0022-5320(62)90050-3. [DOI] [PubMed] [Google Scholar]
  4. Dingman C. W. A convenient program for the rapid calculation of sedimentation coefficients in linear salt or sucrose gradients. Anal Biochem. 1972 Sep;49(1):124–133. doi: 10.1016/0003-2697(72)90249-7. [DOI] [PubMed] [Google Scholar]
  5. Farrell J. A., Harvey J. D., Bellamy A. R. Biophysical studies of reovirus type 3. Virology. 1974 Nov;62(1):145–153. doi: 10.1016/0042-6822(74)90310-9. [DOI] [PubMed] [Google Scholar]
  6. Geddes R., Rapson K. B. Post-mortem degradation of glycogen. FEBS Lett. 1973 May 1;31(3):324–326. doi: 10.1016/0014-5793(73)80132-2. [DOI] [PubMed] [Google Scholar]
  7. Geddes R., Stratton G. C. The influence of lysosomes on glycogen metabolism. Biochem J. 1977 May 1;163(2):193–200. doi: 10.1042/bj1630193. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Harvey J. D. Diffusion coefficients and hydrodynamic radii of three spherical RNA viruses by laser light scattering. Virology. 1973 Nov;56(1):365–368. doi: 10.1016/0042-6822(73)90313-9. [DOI] [PubMed] [Google Scholar]
  9. Harvey J. D., Farrell J. A., Bellamy A. R. Biophysical studies of reovirus type 3. II. Properties of the hydrated particle. Virology. 1974 Nov;62(1):154–160. doi: 10.1016/0042-6822(74)90311-0. [DOI] [PubMed] [Google Scholar]
  10. Kratky O., Leopold H., Stabinger H. The determination of the partial specific volume of proteins by the mechanical oscillator technique. Methods Enzymol. 1973;27:98–110. doi: 10.1016/s0076-6879(73)27007-6. [DOI] [PubMed] [Google Scholar]
  11. Krisman C. R., Parodi A. J. In vitro synthesis of particulate glycogen from uridine diphosphate glucose. 3. Some properties of the product synthesized by muscle glycogen synthetase. Arch Biochem Biophys. 1970 Nov;141(1):228–235. doi: 10.1016/0003-9861(70)90126-8. [DOI] [PubMed] [Google Scholar]
  12. LASKOV R., GROSS J. THE SIZE AND SHAPE OF RAT LIVER GLYCOGEN PREPARED BY PHENOL EXTRACTION. Isr J Med Sci. 1965 Jan;1:26–42. [PubMed] [Google Scholar]
  13. MARTIN R. G., AMES B. N. A method for determining the sedimentation behavior of enzymes: application to protein mixtures. J Biol Chem. 1961 May;236:1372–1379. [PubMed] [Google Scholar]
  14. Mehl J. W., Oncley J. L., Simha R. VISCOSITY AND THE SHAPE OF PROTEIN MOLECULES. Science. 1940 Aug 9;92(2380):132–133. doi: 10.1126/science.92.2380.132. [DOI] [PubMed] [Google Scholar]
  15. Mordoh J., Krisman C. R., Leloir L. F. Further studies on high molecular weight liver glycogen. Arch Biochem Biophys. 1966 Feb;113(2):265–272. doi: 10.1016/0003-9861(66)90186-x. [DOI] [PubMed] [Google Scholar]
  16. ORRELL S. A., BUEDING E. A COMPARISON OF PRODUCTS OBTAINED BY VARIOUS PROCEDURES USED FOR THE EXTRACTION OF GLYCOGEN. J Biol Chem. 1964 Dec;239:4021–4026. [PubMed] [Google Scholar]
  17. Parodi A. J., Krisman C. R., Mordoh J. In vitro synthesis of particulate glycogen from uridine diphosphate glucose. II. Some studies on the growth process. Arch Biochem Biophys. 1970 Nov;141(1):219–227. doi: 10.1016/0003-9861(70)90125-6. [DOI] [PubMed] [Google Scholar]
  18. Wanson J. C., Drochmans P. Rabbit skeletal muscle glycogen. A morphological and biochemical study of glycogen beta-particles isolated by the precipitation-centrifugation method. J Cell Biol. 1968 Jul;38(1):130–150. doi: 10.1083/jcb.38.1.130. [DOI] [PMC free article] [PubMed] [Google Scholar]

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