Skip to main content
Biochemical Journal logoLink to Biochemical Journal
. 1977 May 1;163(2):303–307. doi: 10.1042/bj1630303

Evidence that latent collagenases are enzyme-inhibitor complexes.

A Sellers, E Cartwright, G Murphy, J J Reynolds
PMCID: PMC1164697  PMID: 194584

Abstract

Specific collagenase from the culture media of various rabbit tissues and cells exists in active and latent forms. Latent collagenase is most effectively activated with 4-aminophenylmercuric acetate, a thiol-blocking reagent, strongly suggesting that latent forms are enzyme-inhibitor complexes. A collagenase inhibitor from bone cultures, which may be closely related to the inhibitor of such latent enzyme complexes, was partially characterized.

Full text

PDF
303

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andrews P. Estimation of the molecular weights of proteins by Sephadex gel-filtration. Biochem J. 1964 May;91(2):222–233. doi: 10.1042/bj0910222. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bauer E. A., Eisen A. Z., Jeffrey J. J. Regulation of vertebrate collagenase activity in vivo and in vitro. J Invest Dermatol. 1972 Jul;59(1):50–55. doi: 10.1111/1523-1747.ep12625767. [DOI] [PubMed] [Google Scholar]
  3. Birkedal-Hansen H., Cobb C. M., Taylor R. E., Fullmer H. M. Procollagenase from bovine gingiva. Biochim Biophys Acta. 1976 Mar 11;429(1):229–238. doi: 10.1016/0005-2744(76)90046-2. [DOI] [PubMed] [Google Scholar]
  4. Dayer J. M., Krane S. M., Russell R. G., Robinson D. R. Production of collagenase and prostaglandins by isolated adherent rheumatoid synovial cells. Proc Natl Acad Sci U S A. 1976 Mar;73(3):945–949. doi: 10.1073/pnas.73.3.945. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Harper E., Gross J. Collagenase, procollagenase and activator relationships in tadpole tissue cultures. Biochem Biophys Res Commun. 1972 Sep 5;48(5):1147–1152. doi: 10.1016/0006-291x(72)90830-3. [DOI] [PubMed] [Google Scholar]
  6. Harris E. D., Jr, Krane S. M. Collagenases (first of three parts). N Engl J Med. 1974 Sep 12;291(11):557–563. doi: 10.1056/NEJM197409122911105. [DOI] [PubMed] [Google Scholar]
  7. Harris E. D., Jr, Krane S. M. Collagenases (second of three parts). N Engl J Med. 1974 Sep 19;291(12):605–609. doi: 10.1056/NEJM197409192911205. [DOI] [PubMed] [Google Scholar]
  8. Harris E. D., Jr, Krane S. M. Collagenases (third of three parts). N Engl J Med. 1974 Sep 26;291(13):652–661. doi: 10.1056/NEJM197409262911305. [DOI] [PubMed] [Google Scholar]
  9. Harris E. D., Jr, Reynolds J. J., Werb Z. Cytochalasin B increases collagenase production by cells in vitro. Nature. 1975 Sep 18;257(5523):243–244. doi: 10.1038/257243a0. [DOI] [PubMed] [Google Scholar]
  10. Hook R. M., Hook C. W., Brown S. I. Fibroblast collagenase partial purification and characterization. Invest Ophthalmol. 1973 Oct;12(10):771–776. [PubMed] [Google Scholar]
  11. Horwitz A. L., Crystal R. G. Collagenase from rabbit pulmonary alveolar macrophages. Biochem Biophys Res Commun. 1976 Mar 22;69(2):296–303. doi: 10.1016/0006-291x(76)90521-0. [DOI] [PubMed] [Google Scholar]
  12. Kruze D., Wojtecka E. Activation of leucocyte collagenase proenzyme by rheumatoid synovial fluid. Biochim Biophys Acta. 1972 Dec 28;285(2):436–446. doi: 10.1016/0005-2795(72)90330-3. [DOI] [PubMed] [Google Scholar]
  13. McCroskery P. A., Richards J. F., Harris E. D., Jr Purification and characterization of a collagenase extracted from rabbit tumours. Biochem J. 1975 Oct;152(1):131–142. doi: 10.1042/bj1520131. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Nagai Y. Vertebrate collagenase: further characterization and the significance of its latent form in vivo. Mol Cell Biochem. 1973 Jun 27;1(2):137–145. doi: 10.1007/BF01659325. [DOI] [PubMed] [Google Scholar]
  15. Sakamoto S., Sakamoto M., Goldhaber P., Glimcher M. Collagenase and bone resorption: isolation of collagenase from culture medium containing serum after stimulation of bone resorption by addition of parathyroid hormone extract. Biochem Biophys Res Commun. 1975 Mar 3;63(1):172–178. doi: 10.1016/s0006-291x(75)80026-x. [DOI] [PubMed] [Google Scholar]
  16. Vaes G. The release of collagenase as an inactive proenzyme by bone explants in culture. Biochem J. 1972 Jan;126(2):275–289. doi: 10.1042/bj1260275. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Werb Z., Burleigh M. C. A specific collagenase from rabbit fibroblasts in monolayer culture. Biochem J. 1974 Feb;137(2):373–385. doi: 10.1042/bj1370373. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Werb Z., Reynolds J. J. Purification and properties of a specific collagenase from rabbit synovial fibroblasts. Biochem J. 1975 Dec;151(3):645–653. doi: 10.1042/bj1510645. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Werb Z., Reynolds J. J. Stimulation by endocytosis of the secretion of collagenase and neutral proteinase from rabbit synovial fibroblasts. J Exp Med. 1974 Dec 1;140(6):1482–1497. doi: 10.1084/jem.140.6.1482. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Woolley D. E., Roberts D. R., Evanson J. M. Small molecular weight beta 1 serum protein which specifically inhibits human collagenases. Nature. 1976 May 27;261(5558):325–327. doi: 10.1038/261325a0. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES