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. 1977 Jun 1;163(3):503–509. doi: 10.1042/bj1630503

A map of the cleavage sites for endonuclease AvaI in the chromosome of bacteriophage lambda.

S G Hughes
PMCID: PMC1164730  PMID: 880215

Abstract

The linear order of nine fragments generated by the action of endonuclease AvaI on the DNA of bacteriophage lambda was determined from the altered fragmentation patterns of bacteriophages containing known deletions and of hybrids of bacteriophages lambda and phi80. Digestion of 5'-terminally 32P-labelled bacteriophage-lambda DNA was used to identify the terminal fragments. Measurement of relative fragment lengths permitted rough mapping of the endonuclease-AvaI cleavage sites relative to the ends of the bacteriophage-lambda chromosome. The fragment order was confirmed and the map refined by analysis of the fragmentation of derivative phages containing single cleavage sites for endonuclease EcoRI.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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