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. 1977 Apr 15;164(1):91–97. doi: 10.1042/bj1640091

Studies on the mode of oestrogenic inhibition of hepatic synthesis of alpha2u-globulin and its corresponding messenger ribonucleic acid in rat liver.

A K Roy, D J Dowbenko, M J Schiop
PMCID: PMC1164761  PMID: 69432

Abstract

1. The possible mechanism of the oestrogenic inhibition of the androgen-dependent synthesis of alpha2u-globulin in rat liver was explored by a correlative study of the amounts of alpha2u-globulin, its corresponding mRNA and circulating testosterone in oestrogen-treated male rats. 2. Daily treatments of mature male rats with oestradiol-17beta (10 microgram/100g body wt.) decreased and ultimately stopped the hepatic synthesis of alpha2u-globulin as determined by both hepatic and urinary concentrations of the protein. The oestrogen-mediated decrease in the hepatic synthesis of alpha2u-globulin was correlated with a decrease in the mRNA for this protein. 3. Withdrawal of oestrogen resulted in the recovery of alpha2u-globulin synthesis and an increase in mRNA for alpha2u-globulin. 4. At higher doses of oestradiol-17beta (50 microgram/100g body wt.), synthesis of alpha2u-globulin was totally suppressed. In addition, this treatment resulted in an extended period of androgen-insensitivity during which treatment with androgens induced synthesis of neither alpha2u-globulin nor its corresponding mtrna. 5. it is concluded that the oestrogenic inhibition of alpha2u-globulin synthesis is mediated by an oestrogen-dependent decrease in the hepatic content of translatable mRNA for alpha2u-globulin.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aviv H., Leder P. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408–1412. doi: 10.1073/pnas.69.6.1408. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Harris S. E., Schwartz R. J., Tsai M. J., O'Malley B. W., Roy A. K. Effect of estrogen on gene expression in the chick oviduct. In vitro transcription of the ovalbumin gene in chromatin. J Biol Chem. 1976 Jan 25;251(2):524–529. [PubMed] [Google Scholar]
  4. Kumar M., Roy A. K., Axelrod A. E. Androgenic induction of alpha-2-u-globulin in the rat: requirement of an intact pituitary. Nature. 1969 Jul 26;223(5204):399–400. doi: 10.1038/223399a0. [DOI] [PubMed] [Google Scholar]
  5. Kurtz D. T., Sippel A. E., Ansah-Yiadom R., Feigelson P. Effects of sex hormones on the level of the messenger RNA for the rat hepatic protein alpha 2u globulin. J Biol Chem. 1976 Jun 25;251(12):3594–3598. [PubMed] [Google Scholar]
  6. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  7. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  8. Liang T., Liao S. A very rapid effect of androgen on initiation of protein synthesis in prostate. Proc Natl Acad Sci U S A. 1975 Feb;72(2):706–709. doi: 10.1073/pnas.72.2.706. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lockard R. E., Lingrel J. B. The synthesis of mouse hemoglobin beta-chains in a rabbit reticulocyte cell-free system programmed with mouse reticulocyte 9S RNA. Biochem Biophys Res Commun. 1969 Oct 8;37(2):204–212. doi: 10.1016/0006-291x(69)90720-7. [DOI] [PubMed] [Google Scholar]
  10. Mathews M., Korner A. Mammalian cell-free protein synthesis directed by viral ribonucleic acid. Eur J Biochem. 1970 Dec;17(2):328–338. doi: 10.1111/j.1432-1033.1970.tb01170.x. [DOI] [PubMed] [Google Scholar]
  11. Milin B., Roy A. K. Androgen "receptor" in rat liver: cytosol "receptor" deficiency in pseudohermaphrodite male rats. Nat New Biol. 1973 Apr 25;242(121):248–250. doi: 10.1038/newbio242248a0. [DOI] [PubMed] [Google Scholar]
  12. O'Malley B. W., Means A. R. Female steroid hormones and target cell nuclei. Science. 1974 Feb 15;183(4125):610–620. doi: 10.1126/science.183.4125.610. [DOI] [PubMed] [Google Scholar]
  13. Rhoads R. E., McKnight G. S., Schimke R. T. Quantitative measurement of ovalbumin messenger ribonucleic acid activity. Localization in polysomes, induction by estrogen, and effect of actinomycin D. J Biol Chem. 1973 Mar 25;248(6):2031–2039. [PubMed] [Google Scholar]
  14. Roberts B. E., Paterson B. M. Efficient translation of tobacco mosaic virus RNA and rabbit globin 9S RNA in a cell-free system from commercial wheat germ. Proc Natl Acad Sci U S A. 1973 Aug;70(8):2330–2334. doi: 10.1073/pnas.70.8.2330. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rosen J. M., Woo S. L., Holder J. W., Means A. R., O'Malley B. W. Preparation and preliminary characterization of purified ovalbumin messenger RNA from the hen oviduct. Biochemistry. 1975 Jan 14;14(1):69–78. doi: 10.1021/bi00672a012. [DOI] [PubMed] [Google Scholar]
  16. Rosenfeld G. C., Comstock J. P., Means A. R., O'Malley B. W. Estrogen-induced synthesis of ovalbumin messenger RNA and its translation in a cell-free system. Biochem Biophys Res Commun. 1972 Feb 25;46(4):1695–1703. doi: 10.1016/0006-291x(72)90805-4. [DOI] [PubMed] [Google Scholar]
  17. Roy A. K. Androgen-dependent synthesis of aplha-2u globulin in the rat: role of the pituitary gland. J Endocrinol. 1973 Feb;56(2):295–301. doi: 10.1677/joe.0.0560295. [DOI] [PubMed] [Google Scholar]
  18. Roy A. K., McMinn D. M., Biswas N. M. Estrogenic inhibition of the hepatic synthesis of alpha2u globulin in the rat. Endocrinology. 1975 Dec;97(6):1501–1508. doi: 10.1210/endo-97-6-1501. [DOI] [PubMed] [Google Scholar]
  19. Roy A. K., Milin B. S., McMinn D. M. Androgen receptor in rat liver: hormonal and developmental regulation of the cytoplasmic receptor and its correlation with the androgen-dependent synthesis of alpha2u-globulin. Biochim Biophys Acta. 1974 Jul 4;354(2):213–232. doi: 10.1016/0304-4165(74)90008-7. [DOI] [PubMed] [Google Scholar]
  20. Roy A. K., Neuhaus O. W. Androgenic control of a sex-dependent protein in the rat. Nature. 1967 May 6;214(5088):618–620. doi: 10.1038/214618b0. [DOI] [PubMed] [Google Scholar]
  21. Roy A. K., Neuhaus O. W., Harmison C. R. Preparation and characterization of a sex-dependent rat urinary protein. Biochim Biophys Acta. 1966 Sep 26;127(1):72–81. doi: 10.1016/0304-4165(66)90477-6. [DOI] [PubMed] [Google Scholar]
  22. Roy A. K., Raber D. L. Immunofluorescent localization of 2u -globulin in the hepatic and renal tissues of rat. J Histochem Cytochem. 1972 Feb;20(2):89–96. doi: 10.1177/20.2.89. [DOI] [PubMed] [Google Scholar]
  23. Roy A. K., Schiop M. J., Dowbenko D. J. The role of thyroxine in the regulation of translatable messenger RNA for alpha2u globulin in rat liver. FEBS Lett. 1976 May 1;64(2):396–399. doi: 10.1016/0014-5793(76)80335-3. [DOI] [PubMed] [Google Scholar]
  24. Sippel A. E., Feigelson P., Roy A. K. Hormonal regulation of the hepatic messenger RNA levels for alpha2u globulin. Biochemistry. 1975 Feb 25;14(4):825–829. doi: 10.1021/bi00675a028. [DOI] [PubMed] [Google Scholar]
  25. Tomkins G. M., Gelehrter T. D., Granner D., Martin D., Jr, Samuels H. H., Thompson E. B. Control of specific gene expression in higher organisms. Expression of mammalian genes may be controlled by repressors acting on the translation of messenger RNA. Science. 1969 Dec 19;166(3912):1474–1480. doi: 10.1126/science.166.3912.1474. [DOI] [PubMed] [Google Scholar]
  26. UTIGER R. D., PARKER M. L., DAUGHADAY W. H. Studies on human growth hormone. I. A radio-immunoassay for human growth hormone. J Clin Invest. 1962 Feb;41:254–261. doi: 10.1172/JCI104478. [DOI] [PMC free article] [PubMed] [Google Scholar]

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