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. 1977 Apr 15;164(1):265–267. doi: 10.1042/bj1640265

Proton translocation coupled to electron flow from endogenous substrates to fumarate in anaerobically grown Escherichia coli K12.

S J Gutowski, H Rosenberg
PMCID: PMC1164782  PMID: 18144

Abstract

Observed leads to H+/2e- values for proton translocation during the reduction of fumarate by endogenous substrates in anaerobic cells of Escherichia coli K12 varied with fumarate concentration. This variation was probably due mainly to incomplete fumarate utilization. Under optimum conditions a minimum value for leads to H+/2e- of 1.04+/-0.20 was obtained.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Garland P. B., Downie J. A., Haddock B. A. Proton translocation and the respiratory nitrate reductase of Escherichia coli. Biochem J. 1975 Dec;152(3):547–559. doi: 10.1042/bj1520547. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Gutowski S. J., Rosenberg H. Effects of dicyclohexylcarbodi-imide on proton translocation coupled to fumarate reduction in anaerobically grown cells of Escherichia coli K-12. Biochem J. 1976 Dec 15;160(3):813–816. doi: 10.1042/bj1600813. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Gutowski S. J., Rosenberg H. Energy coupling to active transport in anaerobically grown mutants of Escherichia Coli K12. Biochem J. 1976 Mar 15;154(3):731–734. doi: 10.1042/bj1540731. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gutowski S. J., Rosenberg H. Succinate uptake and related proton movements in Escherichia coli K12. Biochem J. 1975 Dec;152(3):647–654. doi: 10.1042/bj1520647. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Harold F. M. Chemiosmotic interpretation of active transport in bacteria. Ann N Y Acad Sci. 1974 Feb 18;227:297–311. doi: 10.1111/j.1749-6632.1974.tb14395.x. [DOI] [PubMed] [Google Scholar]
  6. Kay W. W., Kornberg H. L. The uptake of C4-dicarboxylic acids by Escherichia coli. Eur J Biochem. 1971 Jan;18(2):274–281. doi: 10.1111/j.1432-1033.1971.tb01240.x. [DOI] [PubMed] [Google Scholar]
  7. Lawford H. G., Haddock B. A. Respiration-driven proton translocation in Escherichia coli. Biochem J. 1973 Sep;136(1):217–220. doi: 10.1042/bj1360217. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Miki K., Lin E. C. Anaerobic energy-yielding reaction associated with transhydrogenation from glycerol 3-phosphate to fumarate by an Escherichia coli system. J Bacteriol. 1975 Dec;124(3):1282–1287. doi: 10.1128/jb.124.3.1282-1287.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Mitchell P., Moyle J. Respiration-driven proton translocation in rat liver mitochondria. Biochem J. 1967 Dec;105(3):1147–1162. doi: 10.1042/bj1051147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mitchell P. Translocations through natural membranes. Adv Enzymol Relat Areas Mol Biol. 1967;29:33–87. doi: 10.1002/9780470122747.ch2. [DOI] [PubMed] [Google Scholar]
  11. Myers W. F., Huang K. Y. Separation of intermediates of the citric acid cycle and related compounds by thin-layer chromatography. Anal Biochem. 1966 Nov;17(2):210–213. doi: 10.1016/0003-2697(66)90199-0. [DOI] [PubMed] [Google Scholar]
  12. Rosenberg H., Cox G. B., Butlin J. D., Gutowski S. J. Metabolite transport in mutants of Escherichia coli K12 defective in electron transport and coupled phosphorylation. Biochem J. 1975 Feb;146(2):417–423. doi: 10.1042/bj1460417. [DOI] [PMC free article] [PubMed] [Google Scholar]

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