Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1977 Jun 15;164(3):601–605. doi: 10.1042/bj1640601

Retained folates in the rat.

P A Barford, R J Staff, J A Blair
PMCID: PMC1164837  PMID: 883955

Abstract

The retention of radioactivity after doses of 14C- and 3H-labelled folic acid is described. Radioactivity was retained in liver, kidney and gut of rats for some time after administration of the dose. The retained radioactivity could not be displaced by large doses of unlabelled folic acid or unlabelled 5-methyltetrahydrofolate. 14C- and 3H-labbelled folates showed similar chromatographic behaviour onion-exchange chromatography to 5-methyltetrahydrofolate, and on ion-exchange and gel-permeation chromatography to synthetic pteroylhepta-gamma-glutamate.

Full text

PDF
601

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BLAKLEY R. L. The reaction of tetrahydropteroylglutamic acid and related hydropteridines with formaldehyde. Biochem J. 1959 Aug;72:707–715. doi: 10.1042/bj0720707. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barford P. A., Blair J. A., Staff F. J. High-molecular-weight folates of rat liver. Biochem Soc Trans. 1976;4(1):70–71. doi: 10.1042/bst0040070. [DOI] [PubMed] [Google Scholar]
  3. Baugh C. M., Krumdieck C. L. Naturally occurring folates. Ann N Y Acad Sci. 1971 Nov 30;186:7–28. [PubMed] [Google Scholar]
  4. Beavon J. R., Blair J. A. The pH-dependent rearrangements of formyltetrahydrofolates and their nutritional implications. Br J Nutr. 1972 Nov;28(3):385–390. doi: 10.1079/bjn19720047. [DOI] [PubMed] [Google Scholar]
  5. Bird O. D., McGlohon V. M., Vaitkus J. W. Naturally occurring folates in the blood and liver of the rat. Anal Biochem. 1965 Jul;12(1):18–35. doi: 10.1016/0003-2697(65)90138-7. [DOI] [PubMed] [Google Scholar]
  6. Blair J. A., Dransfield E. The urinary excretion of orally administered pteroyl-L-glutamic acid by the rat. Biochem J. 1971 Aug;123(5):907–914. doi: 10.1042/bj1230907. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Blair J. A., Saunders K. J. A convenient method for the preparation of dl-5-methyltetrahydrofolic acid (dl-5-methyl-5,6,7,8-tetrahydropteroyl-L-monoglutamic acid). Anal Biochem. 1970 Apr;34(2):376–381. doi: 10.1016/0003-2697(70)90122-3. [DOI] [PubMed] [Google Scholar]
  8. Brody T., Shin Y. S., Stokstad E. L. Rat brain folate identification. J Neurochem. 1976 Aug;27(2):409–413. doi: 10.1111/j.1471-4159.1976.tb12262.x. [DOI] [PubMed] [Google Scholar]
  9. Brown J. P., Davidson G. E., Scott J. M. The identification of the forms of folate found in the liver, kidney and intestine of the monkey and their biosynthesis from exogenous pteroylglutamate (folic acid). Biochim Biophys Acta. 1974 Mar 20;343(1):78–88. doi: 10.1016/0304-4165(74)90240-2. [DOI] [PubMed] [Google Scholar]
  10. Gapski G. R., Whiteley J. M., Huennekens F. M. Hydroxylated derivatives of 5-methyl-5,6,7,8-tetrahydrofolate. Biochemistry. 1971 Jul 20;10(15):2930–2934. doi: 10.1021/bi00791a021. [DOI] [PubMed] [Google Scholar]
  11. HERBERT V. A palatable diet for producing experimental folate deficiency in man. Am J Clin Nutr. 1963 Jan;12:17–20. doi: 10.1093/ajcn/12.1.17. [DOI] [PubMed] [Google Scholar]
  12. Hintze D. N., Farmer J. L. Identification of poly-gamma-glutamyl chain lengths in folates of Bacillus subtilis. J Bacteriol. 1975 Dec;124(3):1236–1239. doi: 10.1128/jb.124.3.1236-1239.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hoffbrand A. V. Synthesis and breakdown of natural folates (folate polyglutamates). Prog Hematol. 1975;9:85–105. [PubMed] [Google Scholar]
  14. Hoffbrand A. V., Tripp E., Lavoie A. Synthesis of folate polyglutamates in human cells. Clin Sci Mol Med. 1976 Jan;50(1):61–68. doi: 10.1042/cs0500061. [DOI] [PubMed] [Google Scholar]
  15. Landon M. J., Eyre D. H., Hytten F. E. Transfer of folate to the fetus. Br J Obstet Gynaecol. 1975 Jan;82(1):12–19. doi: 10.1111/j.1471-0528.1975.tb00556.x. [DOI] [PubMed] [Google Scholar]
  16. Lavoie A., Tripp E., Parsa K., Hoffbrand A. V. Polyglutamate forms of folate in resting and proliferating mammalian tissues. Clin Sci Mol Med. 1975 Jan;48(1):67–73. doi: 10.1042/cs0480067. [DOI] [PubMed] [Google Scholar]
  17. Moran R. G., Werkheiser W. C., Zakrzewski S. F. Folate metabolism in mammalian cells in culture. I Partial characterization of the folate derivatives present in L1210 mouse leukemia cells. J Biol Chem. 1976 Jun 25;251(12):3569–3575. [PubMed] [Google Scholar]
  18. Retief F. P., Du P Heyns A., Oosthuizen M., Van Reenen O. R. In vivo plasma and urine folate binding after ingestation of 3H-folic acid and 14C-methyl-folate. Br J Haematol. 1976 Jul;33(3):415–424. doi: 10.1111/j.1365-2141.1976.tb03559.x. [DOI] [PubMed] [Google Scholar]
  19. Thenen S. W., Stokstad E. L. Effect of methionine on specific folate coenzyme pools in vitamin B 12 deficient and supplemented rats. J Nutr. 1973 Mar;103(3):363–370. doi: 10.1093/jn/103.3.363. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES