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. 1977 Jul 1;165(1):107–110. doi: 10.1042/bj1650107

An evaluation of ways of using equilibrium dialysis to quantify the binding of ligand to macromolecule.

I A Nimmo, G L Atkins, R C Strange, I W Percy-Robb
PMCID: PMC1164874  PMID: 889566

Abstract

1. The effect of systematic error (loss of ligand, complex or macromolecule) on three of the experimental designs by which equilibrium dialysis may be used to quantify the interaction of ligand and macromolecule is examined theoretically, and the design that is least sensitive to systematic error is identified. 2. Thirteen methods for fitting the binding isotherm to experimental data are compared by using them to analyse simulated data containing random error, and the most reliable method is identified.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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