Abstract
Research on high-yielding and biomass of maize hibrids which adaptive to intercropping environment is important in the context of modern agriculture faced with the challenges of climate change. The field evaluation was conducted in Arjasari, West Java, Indonesia, for two seasons in three different cropping systems, namely: maize sole cropping, maize+soybean intercropping and maize+sweet potato intercropping. The evaluation applied a randomized completed block design with three replications. The article provides a data set of Combined Anova Table and biplot graphic of GGE and AMMI. Combined of Anova Table is provided to identify the effect of genotype, environment and their interaction for the traits observed. Thus biplot of GGE and AMMI is provided to identify representative environment and mega-environment for testing and development of hybrid maize; and to evaluate their adaptability in sole cropping as well as in intercropping with soybean and sweetpotato. The data in this article can be utilized by farmers to choose specific or stable maize hybrids for different cropping system.
Keywords: Ammi, Biomass, Field corns, GGE biplot, Yield
Specifications Table
| Subject | Data Article (Agricultural and Biological Science) |
| Specific subject area | Agronomy and Crop Science |
| Data format | Analyzed data |
| Type of data | Table and Figure |
| Data collection | Raw data were taken from observation and measurement in the field. Data collection was done by measuring all plant in each experimental plot. Each plot composed of 60 plants for sole-cropping and 30 for intercropping. The yield in each plot was converted into tons ha-1. The data gathered were vegetative and yield traits. The traits evaluated were plant weight and cob weight with husks (CWH) for biomass and grain yield (GY) for yield. Data collection has been adjusted to match the maize descriptor. |
| Data source location | Samples were taken from 1 location at the Sarana Penelitian Latihan dan Pengembangan Pertanian (SPLPP) Universitas Padjadjaran in Arjasari District, Bandung Regency, West Java, Indonesia. Latitude 7.06112 S, longitude 107.64645 E, and altitude 950 m asl. |
| Data accessibility | Repository name: Mendeley Data Data identification number: DOI: 10.17632/vpschhv5kg.1 Direct URL to data: https://data.mendeley.com/datasets/vpschhv5kg/1 |
| Related research article | – |
1. Value of the Data
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The data in this article can be utilized by farmers to choose specific or stable maize hybrids for different cropping system.
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This data can help researchers, farmers, and industries to select ideal cropping system for breeding maize hybrids in different cropping systems.
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This dataset can be used in different types of research focusing on maize management, from agricultural, germplasm improvement, and environmental perspectives.
2. Background
The development of special-purpose maize requires a continuous and consistent selection process. Selection is an important stage in the genetic improvement of corn plants [1]. Simultaneous selection in a broad environment needs to be done to obtain genotypes that are adaptive, stable and high yielding. This selection can be done by utilizing stability analysis. Stability analysis can explain the effect of G x E interaction in detail. This analysis can also be used to evaluate the adaptability and yield stability of genotypes, which can help them in the difficult task of finding superior genotypes in the context of significant G × E interactions [2].
In addition to genetic stability, analysis is also needed to determine whether the cropping system applied is profitable. Land Equivalent Ratio (LER) values for different cropping systems are used to determine if a cropping system is profitable. LER analysis aims to determine the level of land productivity of intercropping applied compared to the sole cropping system [3]. A LER of more than 1 reveals the economic advantage of intercropping [4]. In addition to this analysis, satellite technology and machine learning models, are possible to predict land productivity and map cropping intensity [5,6]. Intercropping has been widely applied in various scales of agriculture and proven to improve resource use efficiency, increase agricultural productivity, reduce business risk, and reduce negative external influences compared to sole cropping [7]. The objectives of this study were to identify the effect of genotypes, environments, and their interactions (GEIs) on hybrid maize biomass and yield; identify representative environments and mega-environments for testing and development; and evaluate the adaptability of hybrid maize hybrids developed from crosses of elite strains with broad and specific adaptation in three maize cultivation systems in West Java, Indonesia.
3. Data Description
The combined analysis of variance (ANOVA) for hybrid maize evaluated at two seasons and three different cropping systems in Arjasari with the response variables biomass (CWH) and yield (GY) are presented in Table 1. The results of the combined analysis of variance on the tested characters showed that in the tested CWH and GY characters, almost all response variables of hybrid (G), environment (S, C and S × C), and their interaction (G × S, G × C, and G × S × C) had a very significant effect (p < 0.01) except for the CWH character which did not show a significant effect (p > 0.05) on the season (S) and environment (S × C) variables. The results of the combined ANOVA for the two characters tested showed that the coefficient of variance was 19.87 % in CWH and 15.02 % in GY.
Table 1.
Combined ANOVA for CWH and GY of 24 maize hybrids under two seasons.
| Sources | Df | CWH |
GY |
||||
|---|---|---|---|---|---|---|---|
| SS | %SS | SS | %SS | ||||
| Rep | 12 | 24.74 | 0.48 % | 3.6 | 0.18 % | ||
| Season (S) | 1 | 3.83 | 0.07 % | 106.19 | ** | 5.26 % | |
| Cropping System (C) | 2 | 3514.60 | ** | 68.79 % | 1412.49 | ** | 70.00 % |
| S × C | 2 | 0.85 | 0.02 % | 68.44 | ** | 3.39 % | |
| Genotype (G) | 23 | 285.1 | ** | 5.58 % | 37.78 | ** | 1.87 % |
| G × S | 23 | 141.38 | ** | 2.77 % | 66.81 | ** | 3.31 % |
| G × C | 46 | 317.3 | ** | 6.21 % | 94.27 | ** | 4.67 % |
| GEIs (G × C × S) | 46 | 220.04 | ** | 4.31 % | 109.82 | ** | 5.44 % |
| Error | 276 | 601.38 | 11.77 % | 118.41 | 5.87 % | ||
| Total | 431 | 5109.21 | 100.00 % | 2017.81 | ** | 100.00 % | |
| Mean (t.ha−1) | 7.43 | 4.36 | |||||
| Min (t.ha−1) | 2.57 | 1.44 | |||||
| Max (t.ha−1) | 21.11 | 14.15 | |||||
| CV (%) | 19.87 | 15.02 % | |||||
| SD | 3.44 | 2.16 | |||||
SD = standard deviation; CV = Coefficient of variation; df = degree freedom; SS = Sum of square; MS = mean of square; ** p < 0.01.
Biomass (CWH) and yield (GY) for the hybrids tested in two seasons and three different cropping systems averaged 7.43 t.ha−1 and 4.36 t.ha−1, and the minimum values were 2.57 t.ha−1 and 1.44 t.ha−1, while the maximum values were 21.11 t.ha−1 and 14.15 t.ha−1 (Table 3). In CWH characters, the highest difference was shown by the effect of cropping system (C) by 68.79 %, while the effect of season (S) by 0.07 %, genotype (G) by 5.58 %, and GEIs (G × S × C) by 4.31 %. Then in the GY character, the highest difference was shown by the effect of planting system (C) of 70.00 %, while the effect of season (S) was 5.26 %, genotype (G) was 1.87 %, and the interaction of GEIs (G × S × C) was 5.44 %.
Table 3.
High-stable genotype for GY of 24 maize hybrids under three cropping system.
| Gen | GY | rGY | ASV | rASV | GSI | rGSI |
|---|---|---|---|---|---|---|
| H1 | 4.794568 | 2 | 1.85909 | 18 | 20 | 5 |
| H2 | 4.712901 | 3 | 1.5837208 | 15 | 18 | 4 |
| H3 | 5.287778 | 1 | 5.2063954 | 24 | 25 | 12 |
| H4 | 4.286235 | 16 | 1.0168983 | 9 | 25 | 12 |
| H5 | 4.52463 | 5 | 1.9948371 | 20 | 25 | 12 |
| H6 | 4.073333 | 21 | 0.8361593 | 8 | 29 | 19 |
| H7 | 4.173889 | 18 | 1.0237023 | 10 | 28 | 18 |
| H8 | 4.349753 | 12 | 1.8637358 | 19 | 31 | 21 |
| H9 | 4.308518 | 13 | 1.5853866 | 16 | 29 | 19 |
| H10 | 4.230926 | 17 | 0.745642 | 7 | 24 | 10 |
| H11 | 4.303364 | 14 | 2.1327229 | 21 | 35 | 22 |
| H12 | 4.110124 | 19 | 1.6735872 | 17 | 36 | 23 |
| H13 | 4.465864 | 6 | 1.197241 | 11 | 17 | 3 |
| H14 | 4.04963 | 22 | 0.5389847 | 2 | 24 | 10 |
| H15 | 4.287984 | 15 | 0.6993901 | 5 | 20 | 5 |
| H16 | 4.424321 | 8 | 1.377584 | 14 | 22 | 8 |
| H17 | 4.399321 | 9 | 0.3045344 | 1 | 10 | 1 |
| H18 | 4.464198 | 7 | 1.3022106 | 13 | 20 | 5 |
| H19 | 4.377037 | 10 | 1.3002327 | 12 | 22 | 8 |
| H20 | 4.077346 | 20 | 0.7095017 | 6 | 26 | 15 |
| T1 | 4.021049 | 23 | 2.4462294 | 23 | 46 | 24 |
| T2 | 4.684568 | 4 | 2.2056537 | 22 | 26 | 15 |
| T3 | 4.370926 | 11 | 0.5914402 | 4 | 15 | 2 |
| T4 | 3.849568 | 24 | 0.5650185 | 3 | 27 | 17 |
GY = Grain Yield; rCWH = rank of GY; ASV = AMMI stability index; rASV = rank of ASV; GSI = genotype stability index; rGSI = rank of GSI.
Genotype and Genotype x Environment (GGE) Biplot for three cropping systems in two seasons divided into Environment 1 (C1/maize sole crop season 1), Environment 2 (C2/maize + soybean intercropping season 1), Environment 3 (C3/maize + sweet potato season 1),
Environment 4 (C4/maize single crop season 2), Environment 5 (C5/maize + soybean intercropping season 2) and Environment 6 (C6/maize + sweet potato season 2). Visualization of biomass stability (CWH) of hybrid maize using GGE biplot analysis is presented in Fig. 1a, b and c, while yield (GY) is presented in Fig. 2a, b and c.
Fig. 1.
GGE biplot for biomass (CWH) of 24 maize hybrids under three different cropping system: a. ‘Discriminativeness vs representativeness’, b.‘Mean vs stability’, c. ‘Which won where’. Genotype code see Table.
Fig. 2.
GGE biplot for yield (GY) of 24 maize hybrids under three different cropping system: a. ‘Discriminativeness vs representativeness’, b.‘Mean vs stability’, c. ‘Which won where’. Genotype code see Table.
GGE biplot analysis of 24 hybrid maize showed that PC1 and PC2 contributed 48.72 % and 25.21 % respectively to the total variation of hybrid maize yield (Fig. 1a, b and c). Fig. 1a shows the “Discriminativeness vs. representativeness” of GGE biplots for biomass characters. The vector length of each location shows differences. C2, C3, C5 and C6 are type I environments and should not be used as test environments because they have short vectors. While C1 is a suitable environment (type II) because it has the longest vector and a small abscissa angle, this environment tends to have high results and is quite representative for all environments so it is a suitable environment for selecting superior hybrids and as a testing environment. C4 as type III which has a long vector and large abscissa angle cannot be used to select superior genotypes, but can be used to select genotypes that are adaptive or specialized in a particular environment.
Fig. 1b shows the “mean vs stability” of the GGE biplot for biomass characters. Ideal points are located and point to the left of the Y-axis. Nine hybrids were identified in the tested maize population that yielded more than the overall average (left of the Y-axis), namely H12, H3, H1, H11, H4, H16, H2, H18 and H17. In comparison, the other 15 genotypes yielded less than the overall average (right side of the Y-axis). Genotypes H1, H11, H4 and H16 were the most stable hybrids (closest to the X-axis), while genotypes H12 and H3 were unstable.
Fig. 1c shows the “Which won where” view of the GGE biplot for biomass. The analysis shows that the cropping system environment is divided into five sectors. The peak hybrid in each sector is called a vertex indicating that the genotype had the highest yield in each environment within that sector. Sectors consisting of more than one environment form a mega-environment. For example, the first sector is a mega-environment consisting of C1, C2, C3 and C6 with H3 and H17 occupying the vertices. Then the second sector has a mega-environment consisting of C4 and C5 with H12 occupying the vertex. While the other sector consists of H10 and T1 which occupy the top of each sector, but there is no environment in the sector. Therefore, in this study, the ideal genotypes for biomass characters based on which won where GGE biplot are H3, H17 and H12.
Fig. 2a shows the “Discriminativeness vs representativeness” of the GGE biplot for yield characters. C2, C3, C5 and C6 are type I environments and should not be used as testing environments because they have short vector distances that do not represent the tested maize hybrids. Whereas C1 is a suitable environment (type II) due to its strong discriminative and representative properties shown from the vectors that have a small angle to the abscissa and long distance so that this environment can be used to select superior genotypes. C4 is included in the type III environment so that it can be used to select genotypes that are adaptive or specialized in a particular environment.
The ‘Mean vs Stability’ biplot is presented in Fig. 2b. Eight maize hybrids were identified as yielding more than the overall mean and located to the left of the Y-axis, namely H3, H1, T2, H2, H17, T3, H11 and H18. However, the other 16 genotypes are located to the right of the Y-axis and have yields less than the overall average. Genotypes H3, H1, T2 and H2 were the most stable hybrids and were closest to the X-axis, while H11 and H18 were unstable. T2 is the ideal genotype and is close to the ideal point and the X-axis, so it has high yield performance (above the overall average yield) in various sole-cropping and multiple-cropping environments.
Fig. 2c shows the “Which won where” GGE biplot for yield. The analysis shows that the cropping systems environment is divided into six sectors. There is one sector that consists of more than one environment, forming a mega-environment, namely the first sector consisting of C1, C5 and C6 with H3 and H1 as vertices. The second sector has C4 and H11 as vertices. The next sector is C2 with vertex H12. Then the last sector that has an environment consists of C3 with T4 occupying the vertex. While the other sector consists of T1 which occupies the vertex, but there is no environment in the sector. Thus, in this study the ideal genotypes for biomass characters based on which won where GGE biplot are H3, H11, H11 and H12.
The AMMI2 biplots for biomass and yield are presented in Fig. 3a and b which show that there are genotypes that are stable in all test environments and there are specific genotypes in certain environments. In Fig 3a, the widely adapted genotypes based on biomass characteristics were T4, H4, H1, H6, H9, H18 and H20. H14 was found to have environment-specific adaptation in C5 and H19 adapted in C4. Based on the seed results in Fig. 3b, H13, H20 and H17 are widely adapted in all environments. While T4 has environment-specific adaptation in C2.
Fig. 3.
AMMI Biplot of 24 maize hybrids under three different cropping system: a. Biomass (CWH); b. Yield (GY). Genotype code see Table.
High yield stable genotypes on biomass are shown in Table 2. The ranking of genotypes with the highest biomass (rCWH) are H12, H3, H1, T4 and H4. While the ranking of stable genotypes (rASV) are T4, H4, H6, H1 and H9. The ranking of stable genotypes with high biomass (rGSI) are H1, H4, H16, H18 and H6.
Table 2.
High-stable genotype for CWH of 24 maize hybrids under three cropping system.
| Gen | CWH | rCWH | ASV | rASV | GSI | rGSI |
|---|---|---|---|---|---|---|
| H1 | 8.756975 | 3 | 0.2277562 | 4 | 7 | 1 |
| H2 | 7.79801 | 6 | 0.6156969 | 16 | 22 | 7 |
| H3 | 8.981728 | 2 | 1.6107813 | 22 | 24 | 9 |
| H4 | 7.900556 | 5 | 0.1651713 | 2 | 7 | 1 |
| H5 | 7.339649 | 12 | 0.6140148 | 15 | 27 | 13 |
| H6 | 7.244609 | 15 | 0.1818535 | 3 | 18 | 5 |
| H7 | 7.129239 | 16 | 0.485574 | 11 | 27 | 13 |
| H8 | 7.067195 | 17 | 0.5711775 | 14 | 31 | 20 |
| H9 | 6.516049 | 23 | 0.3053621 | 5 | 28 | 17 |
| H10 | 6.740432 | 20 | 1.1629429 | 19 | 39 | 23 |
| H11 | 7.611432 | 8 | 1.1809348 | 20 | 28 | 17 |
| H12 | 9.624568 | 1 | 2.4395253 | 24 | 25 | 11 |
| H13 | 7.401204 | 10 | 0.7551079 | 17 | 27 | 13 |
| H14 | 6.833395 | 18 | 0.456236 | 9 | 27 | 13 |
| H15 | 7.279993 | 14 | 0.475011 | 10 | 24 | 9 |
| H16 | 7.763774 | 7 | 0.3834887 | 7 | 14 | 3 |
| H17 | 7.337333 | 13 | 0.8336439 | 18 | 31 | 20 |
| H18 | 7.390317 | 11 | 0.3435281 | 6 | 17 | 4 |
| H19 | 6.760216 | 19 | 0.5573242 | 13 | 32 | 22 |
| H20 | 6.733796 | 21 | 0.4081656 | 8 | 29 | 19 |
| T1 | 5.936111 | 24 | 1.908563 | 23 | 47 | 24 |
| T2 | 7.517938 | 9 | 0.5359845 | 12 | 21 | 6 |
| T3 | 8.091738 | 4 | 1.3465013 | 21 | 25 | 11 |
| T4 | 6.548452 | 22 | 0.1526591 | 1 | 23 | 8 |
CWH = cob weight with husk (biomass); rCWH = rank of CWH; ASV = AMMI stability index; rASV = rank of ASV; GSI = genotype stability index; rGSI = rank of GSI.
High yield stable genotypes on seed yield are shown in Table 3. The highest yielding genotypes (rGY) were H3, H1, H2, T2 and H5. Ranked stable genotypes (rASV) were H17, H14, T4, T3 and H15. Ranked stable genotypes with high yield (rGSI) were H17, T3, H13, H2, H5.
Land equivalent ratio (LER) of maize+soybean (IC1) and maize+sweet potato (IC2) intercropping systems in season 1 and season 2 for biomass and yield are presented in Table 4. LER of biomass in IC1 in season 1 for all genotypes showed favorable criteria (LER >1) compared to planting in a single maize cropping system, in season 2 only two genotypes showed less favorable, namely H15 and H16. Biomass LER in IC2 in season 1 had four genotypes that showed favorable criteria, namely H7, H20, T1 and T3, as well as in season 2, namely H3, H8, H18 and T4.
Table 4.
LER analysis for CWH and GY of 24 maize hybrids under two intercropping and two seasons.
| Gen |
CWH |
GY |
||||||
|---|---|---|---|---|---|---|---|---|
|
IC1 |
IC2 |
IC1 |
IC2 |
|||||
| Season 1 | Season 2 | Season 1 | Season 2 | Season 1 | Season 2 | Season 1 | Season 2 | |
| H1 | 1.25 | 1.37 | 0.96 | 0.94 | 1.08 | 1.41 | 0.63 | 0.79 |
| H2 | 1.33 | 1.34 | 0.88 | 0.78 | 1.28 | 1.3 | 0.75 | 0.82 |
| H3 | 1.22 | 1.43 | 0.73 | 1.17 | 1.16 | 1.23 | 0.52 | 1.02 |
| H4 | 1.31 | 1.11 | 0.92 | 0.84 | 1.37 | 1.08 | 0.73 | 0.96 |
| H5 | 1.36 | 1.25 | 0.78 | 0.72 | 1.57 | 1.15 | 0.73 | 0.75 |
| H6 | 1.34 | 1.3 | 0.9 | 0.75 | 1.42 | 1.27 | 0.56 | 0.72 |
| H7 | 1.39 | 1.27 | 1.05 | 0.93 | 1.37 | 1.19 | 1.01 | 0.84 |
| H8 | 1.32 | 1.44 | 0.7 | 1.07 | 1.32 | 1.37 | 0.65 | 0.9 |
| H9 | 1.48 | 1.42 | 0.84 | 0.95 | 1.36 | 1.5 | 1.03 | 1.11 |
| H10 | 1.44 | 1.17 | 0.8 | 0.84 | 1.32 | 1.61 | 0.68 | 1.16 |
| H11 | 1.07 | 1.03 | 0.64 | 0.84 | 1.24 | 1.01 | 0.74 | 0.81 |
| H12 | 1.28 | 1.14 | 0.67 | 0.79 | 1.3 | 1.55 | 0.6 | 1.01 |
| H13 | 1.29 | 1.43 | 0.81 | 0.96 | 1.32 | 1.43 | 0.86 | 0.75 |
| H14 | 1.51 | 1.2 | 0.83 | 0.84 | 1.4 | 1.15 | 0.76 | 0.91 |
| H15 | 1.34 | 0.85 | 0.81 | 0.76 | 1.4 | 1.06 | 0.78 | 0.68 |
| H16 | 1.39 | 0.92 | 0.89 | 0.63 | 1.38 | 1.06 | 0.77 | 0.68 |
| H17 | 1.2 | 1.05 | 0.86 | 0.75 | 1.34 | 0.9 | 0.72 | 0.64 |
| H18 | 1.25 | 1.52 | 0.98 | 1.06 | 1.29 | 1.52 | 0.91 | 1.22 |
| H19 | 1.25 | 1.39 | 0.72 | 0.8 | 1.29 | 1.46 | 0.8 | 0.89 |
| H20 | 1.34 | 1.16 | 1.05 | 0.74 | 1.36 | 1.07 | 0.87 | 0.81 |
| T1 | 1.98 | 1.21 | 1.23 | 0.65 | 1.66 | 0.96 | 0.88 | 0.57 |
| T2 | 1.33 | 1.31 | 0.72 | 0.93 | 1.23 | 1.27 | 0.5 | 1.08 |
| T3 | 1.54 | 1.65 | 1.01 | 0.88 | 1.32 | 1.53 | 0.68 | 0.7 |
| T4 | 1.22 | 1.12 | 0.64 | 1.05 | 1.16 | 1.19 | 0.87 | 1.23 |
IC1 = maize+soybean intercropping; and IC2 = maize+sweet potato intercropping.
Furthermore, the LER for seed yield in IC1 in season 1 all genotypes showed favorable criteria (LER>1) compared to planting in a single maize cropping system, while in season 2 only two genotypes showed less favorable criteria, namely genotypes H17 and T1. Then the LER of seed yield in IC2 in season 1 only two genotypes showed favorable criteria, namely H9 and H7, while in season 2 there were 7 genotypes that showed favorable criteria, namely T4, H18, H10, H9, T2, H3 and H12. Furthermore, the ranking of genotypes based on LER for seed yield in IC1 in season 1 was T1, H5, H6, H14 and H15, while in season 2 it was H10, H12, T3, H18 and H9. Then the LER rankings in IC2 in season 1 were H9 and H7, while in season 2 they were T1, H18, H10, H9 and T2.
4. Experimental Design, Materials and Methods
The field evaluation was done in Arjasari, West Java, Indonesia, for two seasons in three different cropping systems, namely: maize sole cropping, maize+soybean intercropping and maize+sweet potato intercropping. Data of seasons, rainfall,temperature, and type of agroclimate in Arjasari is presented in Table 5. The evaluation applied a randomized completed block design with three replications. The hybrids were planted in a 3 × 3 m plot with a spacing of 0.75 × 0.2 m. Data were gathered during harvest, 93 days following planting, using the Descriptor for Maize [8]. Planting and harvesting were done manually. Each plot composed of 60 plants for sole-cropping and 30 for intercropping. The yield in each plot was converted into tons ha-1. The data gathered were vegetative and yield traits. The traits evaluated were plant weight and cob weight with husks (CWH) for biomass and grain yield (GY) for yield. Data collection has been adjusted to match the maize descriptor [8].
Table 5.
Seasons, rainfall,temperature, agroclimate in arjasari.
| Seasons | Rainfall (mm) | Temperature (°C) |
Agroclimate |
||
|---|---|---|---|---|---|
| Min. | Max. | Avg. | |||
|
Season 1 (Rainy to dry season) |
283,2 | 18,2 | 32,2 | 25,7 | AII2. Wet; ; dry month per year 3–7; wet month 5–9; crop index 2; pH 6; ultisol type of soil. |
|
Season 2 (Dry to rainy season) |
237,3 | 19,6 | 30,4 | 24,2 | |
The study used 20 maize hybrids bred by the Plant Breeding Lab, at Padjadjaran University's Faculty of Agriculture (UNPAD) and four commercial hybrids as check varieties. The hybrids have a broad genetic origin (Table 6) [9].
Table 6.
The maize hybrid materials used in the experiment.
| Code | Parental |
Pedigree | ||
|---|---|---|---|---|
| Female | Male | |||
| H1 | DR 4 | × | MDR 7.2.3 | Female is downy mildew resistant (DMR) line, male is mutant line |
| H2 | DR 4 | × | MDR 16.6.14 | Female is DMR line, male is mutant line |
| H3 | DR 5 | × | MDR 18.8.1 | Female is DMR line, male is mutant line |
| H4 | DR 6 | × | DR 7 | Female and male are DMR lines |
| H5 | DR 7 | × | DR 8 | Female and male are DMR lines |
| H6 | DR 8 | × | MDR 18.8.1 | Female is DMR line, male is mutant line |
| H7 | DR 8 | × | DR 9 | Female and male are DMR lines |
| H8 | DR 8 | × | MDR 1.1.3 | Female is DMR line, male is mutant line |
| H9 | DR 10 | × | MDR 9.1.3 | Female is DMR line, male is mutant line |
| H10 | DR 11 | × | DR 16 | Female is DMR line, male is high protein line |
| H11 | DR 14 | × | DR 18 | Female is DMR line, male is high protein line |
| H12 | DR 19 | × | DR 20 | Female and male are high protein lines |
| H13 | MDR 3.1.4 | × | MDR 18.5.1 | Female and male are mutant lines |
| H14 | MDR 3.1.2 | × | MDR 153.14.1 | Female and male are mutant lines |
| H15 | MDR 7.4.3 | × | DR 18 | Female is mutant line, male is high protein line |
| H16 | MDR 7.4.3 | × | MDR 18.8.1 | Female and male are mutant lines |
| H17 | MDR 7.4.3 | × | MDR 1.1.3 | Female and male are mutant lines |
| H18 | MDR 9.1.3 | × | MDR 1.1.3 | Female and male are mutant lines |
| H19 | MDR 18.8.1 | × | MDR 7.1.9 | Female and male are mutant lines |
| H20 | MDR 153.3.2 | × | MDR 8.5.3 | Female and male are mutant lines |
| T1 | Bisi x | Hybrid commercial of Bisi Hybrid | ||
| T2 | Bisi xy | Hybrid commercial of Bisi Hybrid | ||
| T3 | Pertiwi x | Hybrid commercial of Pertiwi Hybrid | ||
| T4 | NK xyz | Hybrid commercial of Monsanto Hybrid | ||
PBtools software [10] was used to calculate the variance components of each trait. The combined ANOVA model for estimating GEIs included:
where Yijkl is the value of hybrids i in plot l, and the value of each replication in environment j;; μ is the grand mean yield; Gi is the effect of hybrid i; Ej is the effect of the environment j; GEij is an effect of genotype by environment interactions on hybrid i and environment j; Rk(j) is the effect of replication k on the location j; Bl(k) is the effect of replication k on plot l; and εijkl is the error effects caused by mutant i in plot l and repeat k of environment j.
The genotype + genotype × environment (GGE) model defines variation due to genotype and genotype-environment interaction (GEI). [11] approximate the GGE biplot model using the following formula:
where Ῡmn; μm; βn; k; λo; αmo and γno; εmn represent the appearance in environment ‘n’ from hybrid ‘m’; a total of average yield; the effect of environment ‘n’; the number of primer components; the singular value from primer component ‘o’; value of hybrid ‘m’ and environment ‘n’ for primer component ‘o’; and the hybrid ‘m’ error in environment ‘n’, respectively. The GGE biplot analysis was estimated using RStudio.
The stability of the AMMI model for evaluating maize hybrid yield is based on [12]:
Where: Yef represent the yield performance of genotype eth in the environment fth, µ is the average of all yield performances from genotypes used, Ge is the genotype eth mean deviation, Ef is the environment fth mean deviation, λk is the square root of the eigenvalue of the PCA axis g, αeg and γfg are the PC score for PCA axis g of the genotype ith and the environment fth, respectively, and ρef is the residual.
AMMI stability value (ASV) is evaluated using the following formula [13]:
Where: The weights assigned to the IPCA 1 and IPCA 2 scores are ss IPCA1 and ss IPCA2, which are calculated by dividing ss IPCA 1 and ss IPCA 2. The AMMI analysis composes of two IPCA ratings for each genotype: IPCA 1 and IPCA 2. Low ASV scores determine maize hybrids that are stable in a variety of conditions.
The genotype stability index (GSI) was calculated using the hybrids’ ASV rank (rASV) and the rank of biomass and yield (rX) features in the three cropping systems using the following formula [1]:
The land equivalent ratio (LER) is counted based on the formula of [4]:
Where ICi represent biomass or yield average each genotype of crop ‘i’ in intercropping; Ci is biomass or yield average each genotype of crop ‘i’ in sole-crop.
Limitations
The field experiment is conducted in one location, therefore, need to confirm in another crop production area to get more additional data.
Ethics Statement
The dataset described in this article does not involve any human subjects, animal experiments, or data collected from social media platforms.
Credit Author Statement
Mansyur: Conceptualization, Data gathering, Validation, Methodology, Writing – draft. Dedi Ruswandi: Conceptualization, Soft ware, Supervision, Resources, Funding acquisition, Methodology, Project administration, Writing – review & editing.
Acknowledgments
The authors would like to express their deepest gratitude to the Ministry of Research, Technology, and Higher Education of the Republic of Indonesia for the Academic Leadership Grand Of Universitas Padjadajaran (Contract Number: 1549/UN6.3.1/PT.00/2023) to Dedi Ruswandi.
Declaration of Competing Interest
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Contributor Information
Mansyur, Email: mansyur@unpad.ac.id.
D. Ruswandi, Email: d.ruswandi@unpad.ac.id.
Data Availability
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