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. 1977 Jul 1;165(1):169–171. doi: 10.1042/bj1650169

The synthesis of tritium-labelled human corticotropin of high specific radioactivity.

D E Brundish, R Wade
PMCID: PMC1164883  PMID: 196594

Abstract

Human [[3,5-3H2]Tyr23]corticotropin-(1-39)-nonatriacontapeptide of specific radioactivity 25.2 Ci/mmol, identical with unlabelled human corticotropin by several criteria, was prepared via the fully protected di-iodotyrosine compound. The latter was synthesized by classical procedures.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baker J. R., Bennett H. P., Hudson A. M., McMartin C., Purdon G. E. On the metabolism of two adrenocorticotrophin analogues. Clin Endocrinol (Oxf) 1976;5 (Suppl):61S–72S. doi: 10.1111/j.1365-2265.1976.tb03816.x. [DOI] [PubMed] [Google Scholar]
  2. Bennett H. P., Bullock G., Lowry P. J., McMartin C., Peters J. Fate of corticotrophins in an isolated adrenal-cell bioassay and decrease of peptide breakdown by cell purification. Biochem J. 1974 Feb;138(2):185–194. doi: 10.1042/bj1380185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bennett H. P., Elliott D. F., Evans B. E., Lowry P. J., McMartin C. Complete amino acid analysis of peptides and proteins after hydrolysis by a mixture of sepharose-bound peptidases. Biochem J. 1972 Sep;129(3):695–701. doi: 10.1042/bj1290695. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brundish D. E., Martin J. R., Wade R. Tritiated peptides. Part 2. Synthesis of two [3,5-3H2-Tyr2]-analogues of corticotrophin. J Chem Soc Perkin 1. 1976;(20):2182–2185. doi: 10.1039/p19760002182. [DOI] [PubMed] [Google Scholar]
  5. Caldwell J., Dring L. G., Williams R. T. Norephedrines as metabolites of ( 14 C)amphetamine in urine in man. Biochem J. 1972 Aug;129(1):23–24. doi: 10.1042/bj1290023. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. DAVIS N. C., SMITH E. L. Purification and some properties of prolidase of swine kidney. J Biol Chem. 1957 Jan;224(1):261–275. [PubMed] [Google Scholar]
  7. Lemaire S., Yamashiro D., Behrens C., Hao Li C. Adrenocorticotropin. 51. Synthesis and properties of analogues of the human hormone with tyrosine residues replaced by 3,5-diiodotyrosine. J Am Chem Soc. 1977 Mar 2;99(5):1577–1580. doi: 10.1021/ja00447a048. [DOI] [PubMed] [Google Scholar]
  8. Lowry P. J., McMartin C., Peters J. Properties of a simplified bioassay for adrenocorticotrophic activity using the steroidogenic response of isolated adrenal cells. J Endocrinol. 1973 Oct;59(1):43–55. doi: 10.1677/joe.0.0590043. [DOI] [PubMed] [Google Scholar]
  9. Sieber P., Rittel W., Riniker B. Die Synthese von menschlichem adrenocorticotropem Hormon ( h -ACTH) mit revidierter Aminosäuresequenz. Helv Chim Acta. 1972;55(4):1243–1266. doi: 10.1002/hlca.19720550420. [DOI] [PubMed] [Google Scholar]

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