Abstract
Pig plasma benzylamine oxidase is a protein containing cupric copper and pyridoxal phosphate. The pyridoxal phosphate is stably linked to the enzyme. Discrepancies in the numbers of active sites per molecule of enzyme are reported in the literature. This paper shows that the fully active pure enzyme contains 3 mol of pyridoxal phosphate per mol, whereas enzymes with a lower specific activity are shown by titration with phenylhydrazine to have a lower pyrdoxal phosphate content.
Full text
PDFSelected References
These references are in PubMed. This may not be the complete list of references from this article.
- BLASCHKO H., BUFFONI F. PYRIDOXAL PHOSPHATE AS A CONSTITUENT OF THE HISTAMINASE (BENZYLAMINE OXIDASE) OF PIG PLASMA. Proc R Soc Lond B Biol Sci. 1965 Aug 24;163:45–60. doi: 10.1098/rspb.1965.0059. [DOI] [PubMed] [Google Scholar]
- BUFFONI F., BLASCHKO H. BENZYLAMINE OXIDASE AND HISTAMINASE: PURIFICATION AND CRYSTALLIZATION OF AN ENZYME FROM PIG PLASMA. Proc R Soc Lond B Biol Sci. 1964 Dec 15;161:153–167. doi: 10.1098/rspb.1964.0086. [DOI] [PubMed] [Google Scholar]
- Buffoni F., Della Corte L. Pig plasma benzylamine oxidase. Adv Biochem Psychopharmacol. 1972;5:133–149. [PubMed] [Google Scholar]
- DIXON M., KLEPPE K. D-AMINO ACID OXIDASE. I. DISSOCIATION AND RECOMBINATION OF THE HOLOENZYME. Biochim Biophys Acta. 1965 Mar 22;96:357–367. doi: 10.1016/0005-2787(65)90556-3. [DOI] [PubMed] [Google Scholar]
- HJERTEN S., LEVIN O., TISELIUS A. Protein chromatography on calcium phosphate columns. Arch Biochem Biophys. 1956 Nov;65(1):132–155. doi: 10.1016/0003-9861(56)90183-7. [DOI] [PubMed] [Google Scholar]
- Lindström A., Pettersson G. Active-site titration of pig-plasma benzylamine oxidase with hydrazine derivatives. Eur J Biochem. 1973 May 2;34(3):564–568. doi: 10.1111/j.1432-1033.1973.tb02796.x. [DOI] [PubMed] [Google Scholar]
- MCCAMAN R. E., MCCAMAN M. W., HUNT J. M., SMITH M. S. MICRODETERMINATION OF MONOAMINE OXIDASE AND 5-HYDROXYTRYPTOPHAN DECARBOXYLASE ACTIVITIES IN NERVOUS TISSUES. J Neurochem. 1965 Jan;12:15–23. doi: 10.1111/j.1471-4159.1965.tb10246.x. [DOI] [PubMed] [Google Scholar]
- TABOR C. W., TABOR H., ROSENTHAL S. M. Purification of amine oxidase from beef plasma. J Biol Chem. 1954 Jun;208(2):645–661. [PubMed] [Google Scholar]
- Taylor C. E., Taylor R. S., Rasmussen C., Knowles P. F. A catalytic mechanism for the enzyme benzylamine oxidase from pig plasma. Biochem J. 1972 Dec;130(3):713–728. doi: 10.1042/bj1300713. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WADA H., SNELL E. E. The enzymatic oxidation of pyridoxine and pyridoxamine phosphates. J Biol Chem. 1961 Jul;236:2089–2095. [PubMed] [Google Scholar]
- WADDELL W. J. A simple ultraviolet spectrophotometric method for the determination of protein. J Lab Clin Med. 1956 Aug;48(2):311–314. [PubMed] [Google Scholar]
- YAMADA H., YASUNOBU K. T. MONOAMINE OXIDASE. IV. NATURE OF THE SECOND PROSTHETIC GROUP OF PLASMA MONOAMINE OXIDASE. J Biol Chem. 1963 Aug;238:2669–2675. [PubMed] [Google Scholar]
- Yamada H., Suzuki H., Ogura Y. Amine oxidases from microorganisms: stoichiometry of the reaction catalyzed by amine oxidase of Aspergillus niger. Adv Biochem Psychopharmacol. 1972;5:185–201. [PubMed] [Google Scholar]