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. 1975 Mar;145(3):545–548. doi: 10.1042/bj1450545

Acetyl-Coenzyme A carboxylase. Role of the prosthetic group in enzyme polymerization.

A D Landman, K Darkshinamurti
PMCID: PMC1165255  PMID: 239688

Abstract

Apo-(acetyl-CoA carboxylase) completely free from the holoenzyme was prepared from biotin-deficient rat adipose tissue by using affinity chromatography. The apoenzyme does not aggregate under conditions favouring the transition of the holoenzyme to the polymeric form. Such transition is possible after the conversion of the apoenzyme into the holoenzyme in vitro, thus demonstrating the requirement of the prosthetic biotinyl group for enzyme activation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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