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. 1975 Feb;146(2):497–499. doi: 10.1042/bj1460497

Subcellular localization of pulmonary antiotensin-converting enzyme (kininase II).

J W Ryan, U S Ryan, D R Schultz, C Whitaker, A Chung
PMCID: PMC1165329  PMID: 168877

Abstract

Goat antibodies to pig lung angiotensin-converting enzyme (kininase II) were conjugated to microperoxidase. Rat lung tissue, previously incubated with non-immune goat serum, was incubated with the antibody-microperoxidase conjugate and then with H2O2 and 3,3-diaminobenzidine. Electron microscopy revealed reaction product on the plasma membrane and caveolae of endothelial cells, especially those of capillaries and venules. These results support the hypothesis that angiotensin I and bradykinin are metabolized by enzymes on the luminal surface of pulmonary endothelial cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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