Skip to main content
Biochemical Journal logoLink to Biochemical Journal
. 1975 Feb;146(2):505–507. doi: 10.1042/bj1460505

Incorporation of L-[1-14C]leucine into protein by liver postmitochondrial supernatant: opposing effects of preincubated nicotinamide-adenine dinucleotide phosphate and 4-dimethylamino-3'-methylazobenzene.

N P Madsen, J E Labuc
PMCID: PMC1165331  PMID: 239694

Abstract

Combination of preincubated drug-metabolizing medium containing NADP+ with a cell-free protein-synthesizing system resulted in marked stimulation of incorporation of L-[1-14C]leucine into protein. Addition of 4-dimethylamino-3'-methylazobenzene, present and previously preincubated in the drug-metabolizing medium, decreased this effect.

Full text

PDF
505

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andrews T. M., Tata J. R. Protein synthesis by membrane-bound and free ribosomes of secretory and non-secretory tissues. Biochem J. 1971 Feb;121(4):683–694. doi: 10.1042/bj1210683. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Blobel G., Potter V. R. Distribution of radioactivity between the acid-soluble pool and the pools of RNA in the nuclear, nonsedimethable and ribosome fractions of rat liver after a single injection of lebaled orotic acid. Biochim Biophys Acta. 1968 Aug 23;166(1):48–57. doi: 10.1016/0005-2787(68)90489-9. [DOI] [PubMed] [Google Scholar]
  3. Jolchine G., Reiss-Husson F. Comparative studies on two reaction center preparations from Rhodopseudomonas speheroides Y. FEBS Lett. 1974 Mar 15;40(1):5–8. doi: 10.1016/0014-5793(74)80881-1. [DOI] [PubMed] [Google Scholar]
  4. Madsen N. P. Use of toluene/triton X-100 scintillation mixture for counting C14-protein radioactivity. Anal Biochem. 1969 Jun;29(3):542–544. doi: 10.1016/0003-2697(69)90341-8. [DOI] [PubMed] [Google Scholar]
  5. Miller J. A. Carcinogenesis by chemicals: an overview--G. H. A. Clowes memorial lecture. Cancer Res. 1970 Mar;30(3):559–576. [PubMed] [Google Scholar]
  6. Pain V. M. Protein synthesis in a postmitochondrial supernatant system from rat liver. An effect of diabetes at the level of peptide chain initiation. FEBS Lett. 1973 Sep 1;35(1):169–172. doi: 10.1016/0014-5793(73)80603-9. [DOI] [PubMed] [Google Scholar]
  7. Richardson A., McGown E., Henderson L. M., Swan P. B. In vitro amino acid incorporation by the post-mitochondrial supernatant from rat liver. Biochim Biophys Acta. 1971 Dec 30;254(3):468–477. doi: 10.1016/0005-2787(71)90881-1. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES