Abstract
Galactosyltransferase and 5'-nucleotidase were assayed in the same reaction mixture, with ovalbumin as exogenous acceptor of (14-C)galactose and with (3-H)AMP as the substrate for the 5'-nucleotidase assay. The substrates and reaction products of either assay had no significant effect on the activity of the other enzyme.
Full text
PDF


Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bergeron J. J., Ehrenreich J. H., Siekevitz P., Palade G. E. Golgi fractions prepared from rat liver homogenates. II. Biochemical characterization. J Cell Biol. 1973 Oct;59(1):73–88. doi: 10.1083/jcb.59.1.73. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Emmelot P., Bos C. J. Studies on plasma membranes. 3. Mg2+-ATPase,(Na+-K+-Mg2+)-ATPase and 5'-nucleotidase activity of plasma membranes isolated from rat liver. Biochim Biophys Acta. 1966 Jul 13;120(3):369–382. doi: 10.1016/0926-6585(66)90304-9. [DOI] [PubMed] [Google Scholar]
- Fleischer B., Fleischer S., Ozawa H. Isolation and characterization of Golgi membranes from bovine liver. J Cell Biol. 1969 Oct;43(1):59–79. doi: 10.1083/jcb.43.1.59. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- McGuire E. J., Jourdian G. W., Carlson D. M., Roseman S. Incorporation of D-galactose into glycoproteins. J Biol Chem. 1965 Oct;240(10):4112–4115. [PubMed] [Google Scholar]
- Schachter H., Jabbal I., Hudgin R. L., Pinteric L., McGuire E. J., Roseman S. Intracellular localization of liver sugar nucleotide glycoprotein glycosyltransferases in a Golgi-rich fraction. J Biol Chem. 1970 Mar 10;245(5):1090–1100. [PubMed] [Google Scholar]
- Spiro M. J., Spiro R. G. Glycoprotein biosynthesis: studies on thyroglobulin. Thyroid galactosyltransferase. J Biol Chem. 1968 Dec 25;243(24):6529–6537. [PubMed] [Google Scholar]
- Wagner R. R., Cynkin M. A. Glycoprotein metabolism: a UDP-galactose-glycoprotein galactosyltransferase of rat serum. Biochem Biophys Res Commun. 1971 Oct 1;45(1):57–62. doi: 10.1016/0006-291x(71)90049-0. [DOI] [PubMed] [Google Scholar]
